O61661

SS
Gene name
grp (CG17161)
Protein name
Serine/threonine-protein kinase grp
Names
EC 2.7.11.1 , Chk1 homolog , Protein grapes
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG17161
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

CHEK1 is a serine/threonine protein kinase and is a key mediator that links the DNA stress responses to the cell cycle. Full-length CHEK1 has only low basal activity when expressed. Truncation of the C-terminal regulatory region activates CHEK1 even in the absence of stress.

Autoinhibitory domains (AIDs)

419-512 (C-terminal autoinhibitory region) SS

Target domain

22-279 (Protein kinase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

160-185 (Activation loop from InterPro)

Target domain

20-279 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for O61661

Entry ID Method Resolution Chain Position Source
AF-O61661-F1 Predicted AlphaFoldDB

No variants for O61661

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O61661

No associated diseases with O61661

4 regional properties for O61661

Type Name Position InterPro Accession
domain Protein kinase domain 22 - 279 IPR000719
active_site Serine/threonine-protein kinase, active site 139 - 151 IPR008271
binding_site Protein kinase, ATP binding site 28 - 51 IPR017441
domain Checkpoint kinase 1, catalytic domain 20 - 279 IPR034670

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
histone H3T11 kinase activity Catalysis of the reaction
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

8 GO annotations of biological process

Name Definition
centrosome separation The process in which duplicated centrosome components move away from each other. The centriole pair within each centrosome becomes part of a separate microtubule organizing center that nucleates a radial array of microtubules called an aster. The two asters move to opposite sides of the nucleus to form the two poles of the mitotic spindle.
DNA damage checkpoint signaling A signal transduction process that contributes to a DNA damage checkpoint.
mitotic DNA replication checkpoint signaling A signal transduction process that contributes to a mitotic DNA replication checkpoint.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of cell cycle Any process that modulates the rate or extent of progression through the cell cycle.
regulation of syncytial blastoderm mitotic cell cycle A cell cycle process that modulates the rate or extent of the progression through the syncytial blastoderm mitotic cell cycle.
spindle assembly The aggregation, arrangement and bonding together of a set of components to form the spindle, the array of microtubules and associated molecules that serves to move duplicated chromosomes apart.
wound healing The series of events that restore integrity to a damaged tissue, following an injury.

12 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P28708 PRR1 Serine/threonine-protein kinase PRR1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P47116 PTK2 Serine/threonine-protein kinase PTK2/STK2 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q8AYC9 CHEK1 Serine/threonine-protein kinase Chk1 Gallus gallus (Chicken) PR
O14757 CHEK1 Serine/threonine-protein kinase Chk1 Homo sapiens (Human) EV
O35280 Chek1 Serine/threonine-protein kinase Chk1 Mus musculus (Mouse) PR
Q91ZN7 Chek1 Serine/threonine-protein kinase Chk1 Rattus norvegicus (Rat) PR
Q93VD3 CIPK23 CBL-interacting serine/threonine-protein kinase 23 Arabidopsis thaliana (Mouse-ear cress) PR
Q9LDI3 CIPK24 CBL-interacting serine/threonine-protein kinase 24 Arabidopsis thaliana (Mouse-ear cress) PR
Q9FJ54 CIPK20 CBL-interacting serine/threonine-protein kinase 20 Arabidopsis thaliana (Mouse-ear cress) PR
O65554 CIPK6 CBL-interacting serine/threonine-protein kinase 6 Arabidopsis thaliana (Mouse-ear cress) PR
Q94CG0 CIPK21 CBL-interacting serine/threonine-protein kinase 21 Arabidopsis thaliana (Mouse-ear cress) PR
Q2V452 CIPK3 CBL-interacting serine/threonine-protein kinase 3 Arabidopsis thaliana (Mouse-ear cress) SS
10 20 30 40 50 60
MAATLTEAGT GPAATREFVE GWTLAQTLGE GAYGEVKLLI NRQTGEAVAM KMVDLKKHPD
70 80 90 100 110 120
AANSVRKEVC IQKMLQDKHI LRFFGKRSQG SVEYIFLEYA AGGELFDRIE PDVGMPQHEA
130 140 150 160 170 180
QRYFTQLLSG LNYLHQRGIA HRDLKPENLL LDEHDNVKIS DFGMATMFRC KGKERLLDKR
190 200 210 220 230 240
CGTLPYVAPE VLQKAYHAQP ADLWSCGVIL VTMLAGELPW DQPSTNCTEF TNWRDNDHWQ
250 260 270 280 290 300
LQTPWSKLDT LAISLLRKLL ATSPGTRLTL EKTLDHKWCN MQFADNERSY DLVDSAAALE
310 320 330 340 350 360
ICSPKAKRQR LQSSAHLSNG LDDSISRNYC SQPMPTMRSD DDFNVRLGSG RSKEDGGDRQ
370 380 390 400 410 420
TLAQEARLSY SFSQPALLDD LLLATQMNQT QNASQNYFQR LVRRMTRFFV TTRWDDTIKR
430 440 450 460 470 480
LVGTIERLGG YTCKFGDDGV VTVSTVDRNK LRLVFKAHII EMDGKILVDC RLSKGCGLEF
490 500 510
KRRFIKIKNA LEDIVLKGPT TWPIAIATNS VP