O54967
SS
Gene name |
Tnk2 (Ack1) |
Protein name |
Activated CDC42 kinase 1 |
Names |
ACK-1 , EC 2.7.10.2 , EC 2.7.11.1 , Non-receptor protein tyrosine kinase Ack , Tyrosine kinase non-receptor protein 2 |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:51789 |
EC number |
2.7.10.2: Protein-tyrosine kinases |
Protein Class |
|
Descriptions
Ack1 belongs to a family of nonreceptor tyrosine kinases. Ack1 is a 120-kDa protein with an N-terminal sterile α-motif (SAM) domain, a kinase domain, an SH3 domain, and a Cdc42-binding domain (CRIB). The large C-terminal portion of Ack1 contains several proline-rich sequences as well as a clathrin-binding motif, a ubiquitin-binding domain, and a region homologous to Mig6. The C-terminal Mig6 homology region (803-880) participates in inhibitory intramolecular interactions with the kinase domain.<br>The addition of Cdc42 (ligand for CRIB domain) or poly-proline peptides (ligands for SH3 domain) did not activate the purified construct of Ack1 in vitro. However, in cells expressing full-length Ack1, the co-expression of Cdc42 did activate Ack1
Autoinhibitory domains (AIDs)
Target domain |
126-385 (Protein kinase domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
269-295 (Activation loop from InterPro)
Target domain |
126-385 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
Autoinhibited structure
Activated structure
1 structures for O54967
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-O54967-F1 | Predicted | AlphaFoldDB |
No variants for O54967
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for O54967 | |||||
No associated diseases with O54967
10 regional properties for O54967
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 126 - 385 | IPR000719 |
| domain | Serine-threonine/tyrosine-protein kinase, catalytic domain | 127 - 384 | IPR001245 |
| domain | SH3 domain | 388 - 448 | IPR001452 |
| active_site | Tyrosine-protein kinase, active site | 248 - 260 | IPR008266 |
| domain | Cdc42 binding domain-like | 450 - 514 | IPR015116 |
| binding_site | Protein kinase, ATP binding site | 132 - 158 | IPR017441 |
| domain | Tyrosine-protein kinase, catalytic domain | 126 - 385 | IPR020635 |
| domain | Mig-6 domain | 790 - 852 | IPR021619 |
| domain | Activated CDC42 kinase 1, UBA domain | 976 - 1019 | IPR030220 |
| domain | TNK(ACK)-like non-receptor tyrosine-protein kinases, SAM domain | 6 - 67 | IPR049587 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.10.2 | Protein-tyrosine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
13 GO annotations of cellular component
| Name | Definition |
|---|---|
| adherens junction | A cell-cell junction composed of the epithelial cadherin-catenin complex. The epithelial cadherins, or E-cadherins, of each interacting cell extend through the plasma membrane into the extracellular space and bind to each other. The E-cadherins bind to catenins on the cytoplasmic side of the membrane, where the E-cadherin-catenin complex binds to cytoskeletal components and regulatory and signaling molecules. |
| axon | The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter. |
| clathrin-coated pit | A part of the endomembrane system in the form of an invagination of a membrane upon which a clathrin coat forms, and that can be converted by vesicle budding into a clathrin-coated vesicle. Coated pits form on the plasma membrane, where they are involved in receptor-mediated selective transport of many proteins and other macromolecules across the cell membrane, in the trans-Golgi network, and on some endosomes. |
| clathrin-coated vesicle | A vesicle with a coat formed of clathrin connected to the membrane via one of the clathrin adaptor complexes. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytoplasmic vesicle membrane | The lipid bilayer surrounding a cytoplasmic vesicle. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| dendrite | A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body. |
| endosome | A vacuole to which materials ingested by endocytosis are delivered. |
| growth cone | The migrating motile tip of a growing neuron projection, where actin accumulates, and the actin cytoskeleton is the most dynamic. |
| neuronal cell body | The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
8 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| metal ion binding | Binding to a metal ion. |
| non-membrane spanning protein tyrosine kinase activity | Catalysis of the reaction |
| protein serine kinase activity | Catalysis of the reactions |
| protein serine/threonine kinase activity | Catalysis of the reactions |
| protein serine/threonine/tyrosine kinase activity | Catalysis of the reactions |
| protein tyrosine kinase activity | Catalysis of the reaction |
| WW domain binding | Binding to a WW domain of a protein, a small module composed of 40 amino acids and plays a role in mediating protein-protein interactions via proline-rich regions. |
6 GO annotations of biological process
| Name | Definition |
|---|---|
| endocytosis | A vesicle-mediated transport process in which cells take up external materials or membrane constituents by the invagination of a part of the plasma membrane to form a new membrane-bounded vesicle. |
| negative regulation of epidermal growth factor receptor signaling pathway | Any process that stops, prevents, or reduces the frequency, rate or extent of epidermal growth factor receptor signaling pathway activity. |
| phosphorylation | The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide. |
| regulation of keratinocyte differentiation | Any process that modulates the frequency, rate or extent of keratinocyte differentiation. |
| signal transduction | The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. |
| spermatid development | The process whose specific outcome is the progression of a spermatid over time, from its formation to the mature structure. |
29 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q17R13 | TNK2 | Activated CDC42 kinase 1 | Bos taurus (Bovine) | SS |
| Q13470 | TNK1 | Non-receptor tyrosine-protein kinase TNK1 | Homo sapiens (Human) | PR |
| Q07912 | TNK2 | Activated CDC42 kinase 1 | Homo sapiens (Human) | EV |
| P48025 | Syk | Tyrosine-protein kinase SYK | Mus musculus (Mouse) | SS |
| P43404 | Zap70 | Tyrosine-protein kinase ZAP-70 | Mus musculus (Mouse) | SS |
| Q64434 | Ptk6 | Protein-tyrosine kinase 6 | Mus musculus (Mouse) | SS |
| P14234 | Fgr | Tyrosine-protein kinase Fgr | Mus musculus (Mouse) | SS |
| P25911 | Lyn | Tyrosine-protein kinase Lyn | Mus musculus (Mouse) | EV |
| P39688 | Fyn | Tyrosine-protein kinase Fyn | Mus musculus (Mouse) | SS |
| P05480 | Src | Proto-oncogene tyrosine-protein kinase Src | Mus musculus (Mouse) | EV |
| P06240 | Lck | Proto-oncogene tyrosine-protein kinase LCK | Mus musculus (Mouse) | SS |
| P16277 | Blk | Tyrosine-protein kinase Blk | Mus musculus (Mouse) | SS |
| P08103 | Hck | Tyrosine-protein kinase HCK | Mus musculus (Mouse) | SS |
| Q04736 | Yes1 | Tyrosine-protein kinase Yes | Mus musculus (Mouse) | SS |
| Q922K9 | Frk | Tyrosine-protein kinase FRK | Mus musculus (Mouse) | SS |
| Q03526 | Itk | Tyrosine-protein kinase ITK/TSK | Mus musculus (Mouse) | SS |
| P35991 | Btk | Tyrosine-protein kinase BTK | Mus musculus (Mouse) | EV |
| P24604 | Tec | Tyrosine-protein kinase Tec | Mus musculus (Mouse) | SS |
| P41242 | Matk | Megakaryocyte-associated tyrosine-protein kinase | Mus musculus (Mouse) | SS |
| P41241 | Csk | Tyrosine-protein kinase CSK | Mus musculus (Mouse) | EV |
| Q9QVP9 | Ptk2b | Protein-tyrosine kinase 2-beta | Mus musculus (Mouse) | PR |
| P34152 | Ptk2 | Focal adhesion kinase 1 | Mus musculus (Mouse) | SS |
| Q99ML2 | Tnk1 | Non-receptor tyrosine-protein kinase TNK1 | Mus musculus (Mouse) | PR |
| P70451 | Fer | Tyrosine-protein kinase Fer | Mus musculus (Mouse) | PR |
| Q6J9G1 | Styk1 | Tyrosine-protein kinase STYK1 | Mus musculus (Mouse) | PR |
| P00520 | Abl1 | Tyrosine-protein kinase ABL1 | Mus musculus (Mouse) | EV |
| Q62270 | Srms | Tyrosine-protein kinase Srms | Mus musculus (Mouse) | SS |
| Q4JIM5 | Abl2 | Tyrosine-protein kinase ABL2 | Mus musculus (Mouse) | SS |
| Q5U2X5 | Tnk2 | Activated CDC42 kinase 1 | Rattus norvegicus (Rat) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MQPEEGTGWL | LELLSEVQLQ | QYFLRLRDDL | NITRLSHFEY | VKNEDLEKIG | MGRPGQRRLW |
| 70 | 80 | 90 | 100 | 110 | 120 |
| EAVKRRKAMC | KRKSWMSKVF | SGKRLEAEFP | SQHSQSTFRK | PSPTPGSLPG | EGTLQSLTCL |
| 130 | 140 | 150 | 160 | 170 | 180 |
| IGEKDLRLLE | KLGDGSFGVV | RRGEWDAPAG | KTVSVAVKCL | KPDVLSQPEA | MDDFIREVNA |
| 190 | 200 | 210 | 220 | 230 | 240 |
| MHSLDHRNLI | RLYGVVLTLP | MKMVTELAPL | GSLLDRLRKH | QGHFLLGTLS | RYAVQVAEGM |
| 250 | 260 | 270 | 280 | 290 | 300 |
| AYLESKRFIH | RDLAARNLLL | ATRDLVKIGD | FGLMRALPQN | DDHYVMQEHR | KVPFAWCAPE |
| 310 | 320 | 330 | 340 | 350 | 360 |
| SLKTRTFSHA | SDTWMFGVTL | WEMFTYGQEP | WIGLNGSQIL | HKIDKEGERL | PRPEDCPQDI |
| 370 | 380 | 390 | 400 | 410 | 420 |
| YNVMVQCWAH | KPEDRPTFVA | LRDFLLEAQP | TDMRALQDFE | EPDKLHIQMN | DVITVIEGRA |
| 430 | 440 | 450 | 460 | 470 | 480 |
| ENYWWRGQNT | RTLCVGPFPR | NVVTSVAGLS | AQDISQPLQN | SFIHTGHGDS | DPRHCWGFPD |
| 490 | 500 | 510 | 520 | 530 | 540 |
| RIDELYLGNP | MDPPDLLSVE | LSTSRPTQHL | GRVKREPPPR | PPQPAIFTQK | TTYDPVSEDP |
| 550 | 560 | 570 | 580 | 590 | 600 |
| DPLSSDFKRL | GLRKPALPRG | LWLAKPSARV | PGTKADRSSG | GEVTLIDFGE | EPVVPTPRPC |
| 610 | 620 | 630 | 640 | 650 | 660 |
| APSLAQLAMD | ACSLLDKTPP | QSPTRALPRP | LHPTPVVDWD | ARPLPPPPAY | DDVAQDEDDF |
| 670 | 680 | 690 | 700 | 710 | 720 |
| EVCSINSTLV | GAGLPAGPSQ | GETNYAFVPE | QAQMPPALED | NLFLPPQGGG | KPPSSVQTAE |
| 730 | 740 | 750 | 760 | 770 | 780 |
| IFQALQQECM | RQLQVPTGQL | TPSPTPGGDD | KPQVPPRVPI | PPRPTRPRVE | LSPAPSGEEE |
| 790 | 800 | 810 | 820 | 830 | 840 |
| TSRWPGPASP | PRVPPREPLS | PQGSRTPSPL | VPPGSSPLPH | RLSSSPGKTM | PTTQSFASDP |
| 850 | 860 | 870 | 880 | 890 | 900 |
| KYATPQVIQA | PGPRAGPCIL | PIVRDGRKVS | STHYYLLPER | PPYLERYQRF | LREAQSPEEP |
| 910 | 920 | 930 | 940 | 950 | 960 |
| AALPVPPLLP | PPSTPAPAAP | TATVRPMPQA | APDPKANFST | NNSNPGARPP | SLRAAARLPQ |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| RGCPGDGQEA | ARPADKVQML | QAMVHGVTTE | ECQAALQSHS | WSVQRAAQYL | KVEQLFGLGL |
| 1030 | 1040 | 1050 | |||
| RPRVECHKVL | EMFDWNLEQA | GCHLLGSCGP | AHHKR |