O54874

SS
Gene name
Cdc42bpa (Pk428)
Protein name
Serine/threonine-protein kinase MRCK alpha
Names
EC 2.7.11.1 , CDC42-binding protein kinase alpha , Myotonic dystrophy kinase-related CDC42-binding kinase alpha , MRCK alpha , Myotonic dystrophy protein kinase-like alpha
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:114116
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

CDC42BPA(MRCK) is a Cdc42-binding serine/threonine kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. A region containing the two distal CC domains (CC2 and CC3; residues 658-930) interacts intramolecularly with the kinase domain and negatively regulates its activity.

Autoinhibitory domains (AIDs)

658-930 (Coiled-coil domains) SS

Target domain

77-343 (Protein kinase domain)

Relief mechanism

Ligand binding

Assay

Accessory elements

218-242 (Activation loop from InterPro)

Target domain

4-412 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

2 structures for O54874

Entry ID Method Resolution Chain Position Source
4AW2 X-ray 170 A A 2-424 PDB
AF-O54874-F1 Predicted AlphaFoldDB

No variants for O54874

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O54874

No associated diseases with O54874

12 regional properties for O54874

Type Name Position InterPro Accession
domain CRIB domain 1571 - 1606 IPR000095
domain Protein kinase domain 77 - 343 IPR000719
domain AGC-kinase, C-terminal 344 - 414 IPR000961
domain Citron homology (CNH) domain 1227 - 1505 IPR001180
domain Pleckstrin homology domain 1082 - 1203 IPR001849
domain Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 1012 - 1063 IPR002219
active_site Serine/threonine-protein kinase, active site 197 - 209 IPR008271
domain Myotonic dystrophy protein kinase, coiled coil 881 - 941 IPR014930
binding_site Protein kinase, ATP binding site 83 - 106 IPR017441
domain Protein kinase, C-terminal 362 - 404 IPR017892
domain Serine/threonine-protein kinase MRCK alpha, catalytic domain 4 - 412 IPR026611
domain KELK-motif containing domain 529 - 608 IPR031597

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Cell projection, lamellipodium
  • Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction
  • This concentration is PH-domain dependent (PubMed:9418861)
  • Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

6 GO annotations of cellular component

Name Definition
actomyosin Any complex of actin, myosin, and accessory proteins.
cell leading edge The area of a motile cell closest to the direction of movement.
cell-cell junction A cell junction that forms a connection between two or more cells of an organism; excludes direct cytoplasmic intercellular bridges, such as ring canals in insects.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoskeleton A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles.
lamellipodium A thin sheetlike process extended by the leading edge of a migrating cell or extending cell process; contains a dense meshwork of actin filaments.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
identical protein binding Binding to an identical protein or proteins.
magnesium ion binding Binding to a magnesium (Mg) ion.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

8 GO annotations of biological process

Name Definition
actin cytoskeleton organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins.
actomyosin structure organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures containing both actin and myosin or paramyosin. The myosin may be organized into filaments.
cell migration The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues.
cytoskeleton organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures.
microtubule cytoskeleton organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising microtubules and their associated proteins.
nuclear migration The directed movement of the nucleus to a specific location within a cell.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of small GTPase mediated signal transduction Any process that modulates the frequency, rate or extent of small GTPase mediated signal transduction.

15 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q28021 ROCK2 Rho-associated protein kinase 2 Bos taurus (Bovine) SS
Q9W1B0 gek Serine/threonine-protein kinase Genghis Khan Drosophila melanogaster (Fruit fly) SS
O75116 ROCK2 Rho-associated protein kinase 2 Homo sapiens (Human) SS
Q9Y5S2 CDC42BPB Serine/threonine-protein kinase MRCK beta Homo sapiens (Human) SS
Q13464 ROCK1 Rho-associated protein kinase 1 Homo sapiens (Human) SS
Q5VT25 CDC42BPA Serine/threonine-protein kinase MRCK alpha Homo sapiens (Human) EV
P70336 Rock2 Rho-associated protein kinase 2 Mus musculus (Mouse) SS
Q80UW5 Cdc42bpg Serine/threonine-protein kinase MRCK gamma Mus musculus (Mouse) PR
Q7TT50 Cdc42bpb Serine/threonine-protein kinase MRCK beta Mus musculus (Mouse) SS
P70335 Rock1 Rho-associated protein kinase 1 Mus musculus (Mouse) SS
Q3UU96 Cdc42bpa Serine/threonine-protein kinase MRCK alpha Mus musculus (Mouse) SS
M3TYT0 ROCK2 Rho-associated protein kinase 2 Sus scrofa (Pig) SS
Q7TT49 Cdc42bpb Serine/threonine-protein kinase MRCK beta Rattus norvegicus (Rat) SS
Q62868 Rock2 Rho-associated protein kinase 2 Rattus norvegicus (Rat) EV
Q63644 Rock1 Rho-associated protein kinase 1 Rattus norvegicus (Rat) SS
10 20 30 40 50 60
MSGEVRLRQL EQFILDGPAQ TNGQCFSVET LLDILICLYD ECNNSPLRRE KNILEYLEWA
70 80 90 100 110 120
KPFTSKVKQM RLHREDFEIL KVIGRGAFGE VAVVKLKNAD KVFAMKILNK WEMLKRAETA
130 140 150 160 170 180
CFREERDVLV NGDSKWITTL HYAFQDDNNL YLVMDYYVGG DLLTLLSKFE DRLPEEMARF
190 200 210 220 230 240
YLAEMVIAID SVHQLHYVHR DIKPDNILMD MNGHIRLADF GSCLKLMEDG TVQSSVAVGT
250 260 270 280 290 300
PDYISPEILQ AMEDGKGRYG PECDWWSLGV CMYEMLYGET PFYAESLVET YGKIMNHKER
310 320 330 340 350 360
FQFPTQVTDV SENAKDLIRR LICSREHRLG QNGIEDFKKH PFFSGIDWDN IRNCEAPYIP
370 380 390 400 410 420
EVSSPTDTSN FDVDDDCLKN SETMPPPTHT AFSGHHLPFV GFTYTSSCVL SDRSCLRVTA
430 440 450 460 470 480
GPTSLDLDVN VQRTLDNNLA TEAYERRIKR LEQEKLELTR KLQESTQTVQ ALQYSTVDGP
490 500 510 520 530 540
LTASKDLEIK SLKEEIEKLR KQVAEVNHLE QQLEEANSVR RELDDAFRQI KAFEKQIKTL
550 560 570 580 590 600
QQEREELNKE LVQASERLKN QSKELKDAHC QRKLAMQEFM EINERLTELH TQKQKLARHV
610 620 630 640 650 660
RDKEEEVDLV MQKAESLRQE LRRAERAKKE LEVHTEALIA EASKDRKLRE QSRHYSKQLE
670 680 690 700 710 720
NELEGLKQKQ ISYSPGICSI EHQQEITKLK TDLEKKSIFY EEEISKREGI HASEIKNLKK
730 740 750 760 770 780
ELHDSEGQQL ALNKEIMVLK DKLEKTRRES QSEREEFENE FKQQYEREKV LLTEENKKLT
790 800 810 820 830 840
SELDKLTSLY ESLSLRNQHL EEEVKDLADK KESVAHWEAQ ITEIIQWVSD EKDARGYLQA
850 860 870 880 890 900
LASKMTEELE ALRNSSLGTR ATDMPWKMRR FAKLDMSARL ELQSALDAEI RAKQAIQEEL
910 920 930 940 950 960
NKVKASNIIT ECKLKDSEKK NLELLSEIEQ LIKDTEELRS EKGVEHRDSQ HSFLAFLNTP
970 980 990 1000 1010 1020
TDALDQFERS PSCTPAGKGR RIADSAPLPV HTPTLRKKGC PASAGFPPKR KTHQFFVKSF
1030 1040 1050 1060 1070 1080
TAPTKCHQCT SLMVGLIRQG CSCEVCGFSC HITCVNKAPT TCPVPPEQTK GPLGIDPQKG
1090 1100 1110 1120 1130 1140
VGTAYEGHVR IPKPAGVKKG WQRALAVVCD FKLFLYDIAE GKASQPSSVI SQVIDMRDEE
1150 1160 1170 1180 1190 1200
FSVSSVLASD VIHASRKDIP CIFRVTASQL SAPSDKCSIL MLADSETERS KWVGVLSELH
1210 1220 1230 1240 1250 1260
KVLKKNKFRD RSVYVPKEAY DSTLPLIKTT QAAAIIDHER VALGNEEGLF VVHVTKDEII
1270 1280 1290 1300 1310 1320
RVGDNKKIHQ IELIPSDQLV AVISGRNRHV RLFPMSALDG RETDFYKLAE TKGCQTIAAG
1330 1340 1350 1360 1370 1380
KVRHGALSCL CVAMKRQVLC YELFQSKTRH RKFKEIQVPC NVQWMAIFSE HLCVGFQSGF
1390 1400 1410 1420 1430 1440
LRYPLNGEGS PCNMLHSNDH TLAFITHQPM DAICAVEISN KEYLLCFSSI GIYTDCQGRR
1450 1460 1470 1480 1490 1500
SRQQELMWPA NPSSCCYNAP YLSIYSENAV DIFDVNSMEW IQTLPLKKVR PLNTEGSLNL
1510 1520 1530 1540 1550 1560
LGLETIRLIY FKNKMAEGDE LVVPETSDNS RKQMVRNINN KRRYSFRVPE EERMQQRREM
1570 1580 1590 1600 1610 1620
LRDPEMRNKL ISNPTNFNHI AHMGPGDGIQ ILKDLPMNPR PQESRTVFSG SVSIPSITKS
1630 1640 1650 1660 1670 1680
RPEPGRSMSA SSGLSARSSA QNGSALKREF SGGSYNTKRQ PMPSPSEGSL SSGGVDQGSD
1690 1700 1710 1720 1730
APVRDYDGED SDSPRHSTAS NSSNLSSPPS PVSPRKTKSL SLESTDRGSW DP