O48963
Gene name |
PHOT1 (JK224, NPH1, RPT1, At3g45780, T6D9_110) |
Protein name |
Phototropin-1 |
Names |
Non-phototropic hypocotyl protein 1, Root phototropism protein 1 |
Species |
Arabidopsis thaliana (Mouse-ear cress) |
KEGG Pathway |
ath:AT3G45780 |
EC number |
2.7.11.1: Protein-serine/threonine kinases |
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
805-815 (Activation loop from InterPro)
Target domain |
663-952 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
849-857 (Activation loop from InterPro)
Target domain |
663-952 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
Autoinhibited structure
Activated structure
3 structures for O48963
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 2Z6C | X-ray | 210 A | A/B | 180-308 | PDB |
| 4HHD | X-ray | 275 A | A/B | 452-615 | PDB |
| AF-O48963-F1 | Predicted | AlphaFoldDB |
85 variants for O48963
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| tmp_3_16818593_T_C | 13 | S>P | No | 1000Genomes | |
| tmp_3_16818642_C_A | 29 | P>Q | No | 1000Genomes | |
| ENSVATH02441716 | 41 | F>Y | No | 1000Genomes | |
| ENSVATH06236999 | 43 | P>L | No | 1000Genomes | |
| tmp_3_16818690_C_T | 45 | P>L | No | 1000Genomes | |
| ENSVATH06237000 | 47 | A>T | No | 1000Genomes | |
| tmp_3_16818717_C_T | 54 | P>L | No | 1000Genomes | |
| tmp_3_16818747_A_T | 64 | Q>L | No | 1000Genomes | |
| tmp_3_16818751_A_C | 65 | Q>H | No | 1000Genomes | |
| ENSVATH02441717 | 67 | P>L | No | 1000Genomes | |
| ENSVATH12539923 | 76 | D>E | No | 1000Genomes | |
| ENSVATH02441719 | 79 | I>S | No | 1000Genomes | |
| tmp_3_16818794_G_A | 80 | A>T | No | 1000Genomes | |
| tmp_3_16818803_A_G | 83 | T>A | No | 1000Genomes | |
| ENSVATH02441720 | 92 | S>L | No | 1000Genomes | |
| tmp_3_16818842_A_C | 96 | I>L | No | 1000Genomes | |
| ENSVATH06237003 | 97 | S>T | No | 1000Genomes | |
| ENSVATH12539924 | 102 | T>I | No | 1000Genomes | |
| tmp_3_16818863_G_A | 103 | A>T | No | 1000Genomes | |
| ENSVATH14374242 | 106 | P>L | No | 1000Genomes | |
| tmp_3_16818872_C_T | 106 | P>S | No | 1000Genomes | |
| ENSVATH02441721 | 111 | V>M | No | 1000Genomes | |
| ENSVATH06237009 | 142 | G>C | No | 1000Genomes | |
| ENSVATH14374243 | 146 | N>I | No | 1000Genomes | |
| tmp_3_16818995_G_A | 147 | D>N | No | 1000Genomes | |
| tmp_3_16819043_T_C | 163 | C>R | No | 1000Genomes | |
| ENSVATH02441727 | 163 | C>Y | No | 1000Genomes | |
| ENSVATH14374265 | 167 | G>D | No | 1000Genomes | |
| ENSVATH02441728 | 174 | V>I | No | 1000Genomes | |
| tmp_3_16819085_G_A | 177 | G>R | No | 1000Genomes | |
| tmp_3_16819092_G_C | 179 | S>T | No | 1000Genomes | |
| ENSVATH12539926 | 205 | T>I | No | 1000Genomes | |
| tmp_3_16819180_T_A | 208 | D>E | No | 1000Genomes | |
| tmp_3_16819861_G_A | 256 | A>T | No | 1000Genomes | |
| ENSVATH02441774 | 268 | K>N | No | 1000Genomes | |
| tmp_3_16819939_C_T | 282 | P>S | No | 1000Genomes | |
| ENSVATH12539990 | 289 | K>I | No | 1000Genomes | |
| tmp_3_16820282_A_C | 305 | E>A | No | 1000Genomes | |
| tmp_3_16820324_A_G | 319 | E>G | No | 1000Genomes | |
| tmp_3_16820502_C_T | 347 | R>W | No | 1000Genomes | |
| ENSVATH14374304 | 354 | N>K | No | 1000Genomes | |
| ENSVATH06237054 | 360 | T>K | No | 1000Genomes | |
| ENSVATH02441802 | 365 | D>N | No | 1000Genomes | |
| ENSVATH02441803 | 366 | E>D | No | 1000Genomes | |
| tmp_3_16820565_C_T | 368 | P>S | No | 1000Genomes | |
| ENSVATH14374315 | 371 | P>S | No | 1000Genomes | |
| tmp_3_16820604_C_T | 381 | P>S | No | 1000Genomes | |
| tmp_3_16820641_A_T | 393 | N>I | No | 1000Genomes | |
| ENSVATH02441808 | 403 | E>V | No | 1000Genomes | |
| ENSVATH06237056 | 414 | M>I | No | 1000Genomes | |
| tmp_3_16820817_G_A | 420 | S>N | No | 1000Genomes | |
| tmp_3_16820847_G_A | 430 | G>E | No | 1000Genomes | |
| ENSVATH06237061 | 433 | E>A | No | 1000Genomes | |
| tmp_3_16820905_G_C | 449 | E>D | No | 1000Genomes | |
| tmp_3_16820913_A_G | 452 | D>G | No | 1000Genomes | |
| tmp_3_16821139_G_C | 495 | S>T | No | 1000Genomes | |
| tmp_3_16821332_A_G | 530 | N>S | No | 1000Genomes | |
| tmp_3_16821684_A_G | 579 | S>G | No | 1000Genomes | |
| tmp_3_16821705_C_T | 586 | R>C | No | 1000Genomes | |
| tmp_3_16821721_A_G | 591 | E>G | No | 1000Genomes | |
| ENSVATH14374324 | 606 | V>M | No | 1000Genomes | |
| ENSVATH12540150 | 632 | V>F | No | 1000Genomes | |
| tmp_3_16822078_G_T | 650 | K>N | No | 1000Genomes | |
| ENSVATH12540152 | 683 | V>I | No | 1000Genomes | |
| ENSVATH06237094 | 695 | D>E | No | 1000Genomes | |
| ENSVATH12540153 | 697 | A>T | No | 1000Genomes | |
| tmp_3_16822611_A_G | 734 | T>A | No | 1000Genomes | |
| ENSVATH02441919 | 744 | P>A | No | 1000Genomes | |
| tmp_3_16822699_A_G | 763 | D>G | No | 1000Genomes | |
| ENSVATH12540204 | 763 | D>N | No | 1000Genomes | |
| ENSVATH00401099 | 781 | C>Y | No | 1000Genomes | |
| tmp_3_16823034_T_C | 814 | S>P | No | 1000Genomes | |
| ENSVATH06237123 | 822 | P>R | No | 1000Genomes | |
| ENSVATH06237125 | 823 | S>N | No | 1000Genomes | |
| ENSVATH06237126 | 836 | Q>E | No | 1000Genomes | |
| ENSVATH06237132 | 882 | M>I | No | 1000Genomes | |
| ENSVATH06237133 | 908 | D>Y | No | 1000Genomes | |
| tmp_3_16823639_A_G | 925 | I>V | No | 1000Genomes | |
| tmp_3_16823678_G_A | 938 | G>S | No | 1000Genomes | |
| ENSVATH02441962 | 940 | F>L | No | 1000Genomes | |
| ENSVATH00401108 | 970 | T>I | No | 1000Genomes | |
| tmp_3_16823890_C_G | 974 | S>C | No | 1000Genomes | |
| ENSVATH12540287 | 976 | E>D | No | 1000Genomes | |
| tmp_3_16823923_A_G | 985 | D>G | No | 1000Genomes | |
| tmp_3_16823926_C_T | 986 | P>L | No | 1000Genomes |
No associated diseases with O48963
9 regional properties for O48963
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | PAS domain | 184 - 305 | IPR000014-1 |
| domain | PAS domain | 464 - 580 | IPR000014-2 |
| domain | PAS-associated, C-terminal | 258 - 312 | IPR000700-1 |
| domain | PAS-associated, C-terminal | 536 - 590 | IPR000700-2 |
| domain | Protein kinase domain | 663 - 952 | IPR000719 |
| repeat | PAC motif | 261 - 303 | IPR001610-1 |
| repeat | PAC motif | 539 - 581 | IPR001610-2 |
| active_site | Serine/threonine-protein kinase, active site | 784 - 796 | IPR008271 |
| binding_site | Protein kinase, ATP binding site | 669 - 692 | IPR017441 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.11.1 | Protein-serine/threonine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
6 GO annotations of cellular component
| Name | Definition |
|---|---|
| cell surface | The external part of the cell wall and/or plasma membrane. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytoplasmic side of plasma membrane | The leaflet the plasma membrane that faces the cytoplasm and any proteins embedded or anchored in it or attached to its surface. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| plant-type vacuole | A closed structure that is completely surrounded by a unit membrane, contains liquid, and retains the same shape regardless of cell cycle phase. An example of this structure is found in Arabidopsis thaliana. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
9 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| blue light photoreceptor activity | The function of absorbing and responding to electromagnetic radiation with a wavelength of approximately 400-470nm. The response may involve a change in conformation. |
| FMN binding | Binding to flavin mono nucleotide. Flavin mono nucleotide (FMN) is the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes. |
| identical protein binding | Binding to an identical protein or proteins. |
| kinase activity | Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule. |
| mRNA binding | Binding to messenger RNA (mRNA), an intermediate molecule between DNA and protein. mRNA includes UTR and coding sequences, but does not contain introns. |
| protein kinase activity | Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP. |
| protein serine kinase activity | Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate. |
| protein serine/threonine kinase activity | Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate. |
10 GO annotations of biological process
| Name | Definition |
|---|---|
| chloroplast accumulation movement | The relocation process in which chloroplasts in photosynthetic cells move toward a brighter area in a cell to optimize photosynthesis. |
| chloroplast avoidance movement | The relocation process in which chloroplasts in photosynthetic cells avoid strong light and move away from it in order to preserve the photosynthetic machinery. |
| circadian rhythm | Any biological process in an organism that recurs with a regularity of approximately 24 hours. |
| negative regulation of anion channel activity by blue light | Any process in which blue light stops, prevents, or reduces the frequency, rate, or extent of the anion channel activity. |
| phototropism | The movement of an organism, or part of an organism, in response to a light stimulus, usually toward or away from it. |
| protein autophosphorylation | The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation). |
| protein phosphorylation | The process of introducing a phosphate group on to a protein. |
| regulation of proton transport | Any process that modulates the frequency, rate or extent of proton transport into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. |
| regulation of stomatal movement | Any process that modulates the frequency, rate or extent of stomatal movement. |
| response to blue light | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a blue light stimulus. Blue light is electromagnetic radiation with a wavelength of between 440 and 500nm. |
20 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P21146 | GRK2 | Beta-adrenergic receptor kinase 1 | Bos taurus (Bovine) | PR |
| P26818 | GRK3 | Beta-adrenergic receptor kinase 2 | Bos taurus (Bovine) | PR |
| P43249 | GRK5 | G protein-coupled receptor kinase 5 | Bos taurus (Bovine) | SS |
| P25098 | GRK2 | Beta-adrenergic receptor kinase 1 | Homo sapiens (Human) | PR |
| P35626 | GRK3 | Beta-adrenergic receptor kinase 2 | Homo sapiens (Human) | PR |
| P43250 | GRK6 | G protein-coupled receptor kinase 6 | Homo sapiens (Human) | EV |
| P34947 | GRK5 | G protein-coupled receptor kinase 5 | Homo sapiens (Human) | EV |
| P32298 | GRK4 | G protein-coupled receptor kinase 4 | Homo sapiens (Human) | SS |
| Q15835 | GRK1 | Rhodopsin kinase GRK1 | Homo sapiens (Human) | PR |
| Q9HBY8 | SGK2 | Serine/threonine-protein kinase Sgk2 | Homo sapiens (Human) | SS |
| Q96BR1 | SGK3 | Serine/threonine-protein kinase Sgk3 | Homo sapiens (Human) | SS |
| O00141 | SGK1 | Serine/threonine-protein kinase Sgk1 | Homo sapiens (Human) | PR |
| Q9ERE3 | Sgk3 | Serine/threonine-protein kinase Sgk3 | Mus musculus (Mouse) | PR |
| P26817 | Grk2 | Beta-adrenergic receptor kinase 1 | Rattus norvegicus (Rat) | PR |
| P26819 | Grk3 | Beta-adrenergic receptor kinase 2 | Rattus norvegicus (Rat) | PR |
| Q62833 | Grk5 | G protein-coupled receptor kinase 5 | Rattus norvegicus (Rat) | SS |
| P97711 | Grk6 | G protein-coupled receptor kinase 6 | Rattus norvegicus (Rat) | SS |
| O64682 | PID | Protein kinase PINOID | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9LUL2 | WAG2 | Serine/threonine-protein kinase WAG2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SUA3 | D6PKL1 | Serine/threonine-protein kinase D6PKL1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MEPTEKPSTK | PSSRTLPRDT | RGSLEVFNPS | TQLTRPDNPV | FRPEPPAWQN | LSDPRGTSPQ |
| 70 | 80 | 90 | 100 | 110 | 120 |
| PRPQQEPAPS | NPVRSDQEIA | VTTSWMALKD | PSPETISKKT | ITAEKPQKSA | VAAEQRAAEW |
| 130 | 140 | 150 | 160 | 170 | 180 |
| GLVLKTDTKT | GKPQGVGVRN | SGGTENDPNG | KKTTSQRNSQ | NSCRSSGEMS | DGDVPGGRSG |
| 190 | 200 | 210 | 220 | 230 | 240 |
| IPRVSEDLKD | ALSTFQQTFV | VSDATKPDYP | IMYASAGFFN | MTGYTSKEVV | GRNCRFLQGS |
| 250 | 260 | 270 | 280 | 290 | 300 |
| GTDADELAKI | RETLAAGNNY | CGRILNYKKD | GTSFWNLLTI | APIKDESGKV | LKFIGMQVEV |
| 310 | 320 | 330 | 340 | 350 | 360 |
| SKHTEGAKEK | ALRPNGLPES | LIRYDARQKD | MATNSVTELV | EAVKRPRALS | ESTNLHPFMT |
| 370 | 380 | 390 | 400 | 410 | 420 |
| KSESDELPKK | PARRMSENVV | PSGRRNSGGG | RRNSMQRINE | IPEKKSRKSS | LSFMGIKKKS |
| 430 | 440 | 450 | 460 | 470 | 480 |
| ESLDESIDDG | FIEYGEEDDE | ISDRDERPES | VDDKVRQKEM | RKGIDLATTL | ERIEKNFVIT |
| 490 | 500 | 510 | 520 | 530 | 540 |
| DPRLPDNPII | FASDSFLELT | EYSREEILGR | NCRFLQGPET | DLTTVKKIRN | AIDNQTEVTV |
| 550 | 560 | 570 | 580 | 590 | 600 |
| QLINYTKSGK | KFWNIFHLQP | MRDQKGEVQY | FIGVQLDGSK | HVEPVRNVIE | ETAVKEGEDL |
| 610 | 620 | 630 | 640 | 650 | 660 |
| VKKTAVNIDE | AVRELPDANM | TPEDLWANHS | KVVHCKPHRK | DSPPWIAIQK | VLESGEPIGL |
| 670 | 680 | 690 | 700 | 710 | 720 |
| KHFKPVKPLG | SGDTGSVHLV | ELVGTDQLFA | MKAMDKAVML | NRNKVHRARA | EREILDLLDH |
| 730 | 740 | 750 | 760 | 770 | 780 |
| PFLPALYASF | QTKTHICLIT | DYYPGGELFM | LLDRQPRKVL | KEDAVRFYAA | QVVVALEYLH |
| 790 | 800 | 810 | 820 | 830 | 840 |
| CQGIIYRDLK | PENVLIQGNG | DISLSDFDLS | CLTSCKPQLL | IPSIDEKKKK | KQQKSQQTPI |
| 850 | 860 | 870 | 880 | 890 | 900 |
| FMAEPMRASN | SFVGTEEYIA | PEIISGAGHT | SAVDWWALGI | LMYEMLYGYT | PFRGKTRQKT |
| 910 | 920 | 930 | 940 | 950 | 960 |
| FTNVLQKDLK | FPASIPASLQ | VKQLIFRLLQ | RDPKKRLGCF | EGANEVKQHS | FFKGINWALI |
| 970 | 980 | 990 | |||
| RCTNPPELET | PIFSGEAENG | EKVVDPELED | LQTNVF |