O45818

SS
Gene name
dkf-2 (T25E12.4)
Protein name
Serine/threonine-protein kinase dkf-2
Names
EC 2.7.11.13 , D kinase family-2
Species
Caenorhabditis elegans
KEGG Pathway
cel:CELE_T25E12.4
EC number
2.7.11.13: Protein-serine/threonine kinases
Protein Class

Descriptions

Protein kinase D (PKD) is a member of Ser/Thr kinases and plays a major role in Golgi function and organization. The PH domain within the PKD inhibits the function of the PKD. When PH domain is phosphorylated or deleted, the function of PKD is recovered.

Autoinhibitory domains (AIDs)

624-728 (PH domain) SS

Target domain

770-1026 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

913-935 (Activation loop from InterPro)

Target domain

770-1026 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for O45818

Entry ID Method Resolution Chain Position Source
AF-O45818-F1 Predicted AlphaFoldDB

No variants for O45818

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O45818

No associated diseases with O45818

10 regional properties for O45818

Type Name Position InterPro Accession
domain Protein kinase domain 770 - 1026 IPR000719
domain Pleckstrin homology domain 624 - 728 IPR001849
domain Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 320 - 372 IPR002219-1
domain Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 473 - 524 IPR002219-2
active_site Serine/threonine-protein kinase, active site 889 - 901 IPR008271
binding_site Protein kinase, ATP binding site 776 - 799 IPR017441
domain Diacylglycerol/phorbol-ester binding 471 - 485 IPR020454-1
domain Diacylglycerol/phorbol-ester binding 487 - 496 IPR020454-2
domain Diacylglycerol/phorbol-ester binding 500 - 511 IPR020454-3
domain Diacylglycerol/phorbol-ester binding 512 - 524 IPR020454-4

Functions

Description
EC Number 2.7.11.13 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Membrane
  • Translocation to the cell membrane is required for kinase activation
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

6 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoplasmic side of plasma membrane The leaflet the plasma membrane that faces the cytoplasm and any proteins embedded or anchored in it or attached to its surface.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
serine/threonine protein kinase complex A protein complex which is capable of protein serine/threonine kinase activity.

7 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
diacylglycerol-dependent serine/threonine kinase activity Catalysis of the reaction
metal ion binding Binding to a metal ion.
protein homodimerization activity Binding to an identical protein to form a homodimer.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

8 GO annotations of biological process

Name Definition
defense response to Gram-negative bacterium Reactions triggered in response to the presence of a Gram-negative bacterium that act to protect the cell or organism.
defense response to Gram-positive bacterium Reactions triggered in response to the presence of a Gram-positive bacterium that act to protect the cell or organism.
determination of adult lifespan The pathways that regulate the duration of the adult phase of the life-cycle of an animal.
innate immune response Innate immune responses are defense responses mediated by germline encoded components that directly recognize components of potential pathogens.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
phospholipase C-activating G protein-coupled receptor signaling pathway A G protein-coupled receptor signaling pathway in which the signal is transmitted via the activation of phospholipase C (PLC) and a subsequent increase in the intracellular concentration of inositol trisphosphate (IP3) and diacylglycerol (DAG).
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
protein import into nucleus The directed movement of a protein from the cytoplasm to the nucleus.

8 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q15139 PRKD1 Serine/threonine-protein kinase D1 Homo sapiens (Human) EV
Q9BZL6 PRKD2 Serine/threonine-protein kinase D2 Homo sapiens (Human) SS
O94806 PRKD3 Serine/threonine-protein kinase D3 Homo sapiens (Human) SS
Q62101 Prkd1 Serine/threonine-protein kinase D1 Mus musculus (Mouse) SS
Q8BZ03 Prkd2 Serine/threonine-protein kinase D2 Mus musculus (Mouse) SS
Q8K1Y2 Prkd3 Serine/threonine-protein kinase D3 Mus musculus (Mouse) SS
Q5XIS9 Prkd2 Serine/threonine-protein kinase D2 Rattus norvegicus (Rat) SS
Q9WTQ1 Prkd1 Serine/threonine-protein kinase D1 Rattus norvegicus (Rat) SS
10 20 30 40 50 60
MDANDYPRLY YTSMPSSSTS MVTTTRFSTS FSPSIPCHRQ ENFRRHSTSA LAKRNGSESE
70 80 90 100 110 120
KSAEIRENPD EIEVSRVSGR DSSLAFYTTA HETSSMLSRD SRDETLTPNE HIHQASSRRV
130 140 150 160 170 180
SANDSVFEDG YVDFVDEPRE HGSRRKSFEK FTDRNGEEKE GRVFELSTPQ PTREAAPGAH
190 200 210 220 230 240
QFQLPTLLVT STPTTVFDHS DDEVWTPYRP PPNREYSSSS EPMMGDLTFR LQSGIHKKSI
250 260 270 280 290 300
AVEGTEIALR DLRNEALQFI KEIYPEKGCS SLEDYILLYK HDLRSINILQ LITTSSDVTD
310 320 330 340 350 360
GTLVEVVIGS CPQNERIVVH PHTLFVHSYK VPTFCDFCGE LLFGLVKQGL KCFGCGLNYH
370 380 390 400 410 420
KRCASKIPNN CNGSKQRRPS AIPLSPSNSN ILNLNERRHS RRESCLEALD AARPSSTLGG
430 440 450 460 470 480
AATPNIFITS DDCGDAVGGN YLQMPRKDRS CSWSGRPLWM EIAEATRVKL QVPHTFQVHS
490 500 510 520 530 540
YKLPTVCQHC KKLLKGLIRQ GMQCRDCKYN CHKKCSEHVA KDCSGNTKAS QFFLGSQADD
550 560 570 580 590 600
GASEDRDDDL SLRSGSGAHK KAQNTPSAPL QGSEGSGSPG PVVSFAANAL SNMPDDDVIS
610 620 630 640 650 660
SESANIPLMR VVMSKKQTKR KNNKLLKEGW IVHYTDQQNM RKKHYWRLDT KGITMYQDEN
670 680 690 700 710 720
TTRYYKEIPL NEILNVSMSP PDKTADYLFE IRTGVCVYFI SGSPSDEKGS SLDAQSWTTA
730 740 750 760 770 780
IQSALMPVTP QSSVVGGKRI DKLKVPTEGE TGHLGAKIQT EHEFSQLYQI FAEEVLGSGQ
790 800 810 820 830 840
FGTVYGGIHR RNGQHVAVKL IDKLKFPPNK EDLLRAEVQI LEKVDHPGVV HFMQMLETTD
850 860 870 880 890 900
RIFVVMEKLK GDMLEMILSS EKGRLSERTT QFLVAQILEA LRYLHHLNIV HCDLKPENIL
910 920 930 940 950 960
LNSNSDFPQV KLCDFGFARI IGEKSFRRSV VGTPAYLAPE VLRNKGFNRS LDMWSVGVIV
970 980 990 1000 1010 1020
YVSLSGTFPF NEDEDINDQI QNAEFMYPPT PWKEISENAI EFINGLLQVK MSKRYTVTKA
1030 1040 1050 1060
QSQIWMQNYT LWSDLRVLEK AVGQRFVTHE SDDVRWQAYE KEHNVTPVYV