O42184

SS
Gene name
CLIP1 (RSN)
Protein name
CAP-Gly domain-containing linker protein 1
Names
Cytoplasmic linker protein 170 , CLIP-170 , Restin
Species
Gallus gallus (Chicken)
KEGG Pathway
gga:395784
EC number
Protein Class

Descriptions

Cytoplasmic linker protein (CLIP-170 or CLIP1) is a prototype of the plus end-tracking proteins that regulate microtubule dynamics. The CAP-Gly-2 domain possesses a basic groove and directly binds the EExEEY/F motif of the C-terminal acidic-tail ends of α-tubulin. The C-terminal zinc knuckle domains of CLIP-170 bind the basic groove to inhibit the binding to the acidic tails of α-tubulin. Once CLIP-170 binds microtubule, the released zinc knuckle domain may serve to recruit dynein to the plus end by interacting with the dynactin subunit p150Glued and LIS1.

Autoinhibitory domains (AIDs)

1371-1427 (C-terminal zinc knuckle domains) SS

Target domain

61-282 (CAP-Gly domain)

Relief mechanism

Others

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for O42184

Entry ID Method Resolution Chain Position Source
AF-O42184-F1 Predicted AlphaFoldDB

No variants for O42184

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O42184

No associated diseases with O42184

4 regional properties for O42184

Type Name Position InterPro Accession
domain CAP Gly-rich domain 61 - 126 IPR000938-1
domain CAP Gly-rich domain 217 - 282 IPR000938-2
domain CLIP1, zinc knuckle 1371 - 1388 IPR032108-1
domain CLIP1, zinc knuckle 1411 - 1427 IPR032108-2

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Cytoplasm, cytoskeleton
  • Cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side
  • Cell projection, ruffle
  • Localizes to microtubule plus ends
  • Localizes preferentially to the ends of tyrosinated microtubules
  • Accumulates in plasma membrane regions with ruffling and protrusions
  • Associates with the membranes of intermediate macropinocytic vesicles
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
cytoplasmic vesicle membrane The lipid bilayer surrounding a cytoplasmic vesicle.
microtubule plus-end The growing (plus) end of a microtubule. In vitro, microtubules polymerize more quickly at the plus end than at the minus end. In vivo, microtubule growth occurs only at the plus end, and the plus end switches between periods of growth and shortening, a behavior known as dynamic instability.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
ruffle Projection at the leading edge of a crawling cell; the protrusions are supported by a microfilament meshwork.

3 GO annotations of molecular function

Name Definition
metal ion binding Binding to a metal ion.
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.
microtubule plus-end binding Binding to the plus end of a microtubule.

2 GO annotations of biological process

Name Definition
cytoplasmic microtubule organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of structures formed of microtubules and associated proteins in the cytoplasm of a cell.
positive regulation of microtubule polymerization Any process that activates or increases the frequency, rate or extent of microtubule polymerization.

4 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q9VJE5 CLIP-190 Restin homolog Drosophila melanogaster (Fruit fly) SS
P30622 CLIP1 CAP-Gly domain-containing linker protein 1 Homo sapiens (Human) EV
Q922J3 Clip1 CAP-Gly domain-containing linker protein 1 Mus musculus (Mouse) SS
Q9JK25 Clip1 CAP-Gly domain-containing linker protein 1 Rattus norvegicus (Rat) EV
10 20 30 40 50 60
MSMLKPSGLK APSKTIKHGS TLLKAPASVA TAPAEKAPSS EKSSSTTTAD AHDDFVDDFR
70 80 90 100 110 120
VGERVWVNGN KPGFIQFLGE TQFAPGQWAG IVLDEPIGKN DGSVAGVRYF QCEPLRGIFT
130 140 150 160 170 180
RPSKLSRKVL TEDEANGTQT AHASRATSPT STSTASAVSA SPAALLPSGI PQKTSPLAAK
190 200 210 220 230 240
EHSTPSQFSN LSKTASGSVS NLSEAGSLKK GERELKIGDR VLVGGTKAGV VRFLGETDFA
250 260 270 280 290 300
KGEWCGVELD EPLGKNDGAV AGTRYFQCQP RYGLFAPVHK VTKIGFPSTT PAKAKTTVRK
310 320 330 340 350 360
VVATPAALKR SPSASSLSSL SSVASSVSSK PSRTGLLTET SSRYARKISG TTALQEALKE
370 380 390 400 410 420
KQQHIEQLLA ERDLERAEVA KATSHVGEIE QELALVRDGH DRHVLEMEAK MDQLRAMVEA
430 440 450 460 470 480
ADREKVELLN QLEEEKRKVE DLQFRVEEES ITKGDLETQT KLEHARIKEL EQSLLFEKTK
490 500 510 520 530 540
ADKLQRELED TRVATVSEKS RIMELERDLA LRVKEVAELR GRLESSKHID DVDTSLSLLQ
550 560 570 580 590 600
EISSLQEKMA AAGKEHQREM SSLKEKFESS EEALRKEIKT LSASNERMGK ENESLKTKLD
610 620 630 640 650 660
HANKENSDVI ELWKSKLESA IASHQQAMEE LKVSFNKGVG AQTAEFAELK TQMEKVKLDY
670 680 690 700 710 720
ENEMSNLKLK QENEKSQHLK EIEALKAKLL EVTEEKEQTL ENLKAKLESV EDQHLVEMED
730 740 750 760 770 780
TLNKLQEAEI KVKELDVLQA KCNEQTKLIG SLTQQIRASE EKLLDLAALQ KANSEGKLEI
790 800 810 820 830 840
QKLSEQLQAA EKQIQNLETE KVSNLTKELQ GKEQKLLDLE KNLSAVNQVK DSLEKELQLL
850 860 870 880 890 900
KEKFTSAVDG AENAQRAMQE TINKLNQKEE QFALMSSELE QLKSNLTVME TKLKEREERE
910 920 930 940 950 960
QQLTEAKVKL ENDIAEIMKS SGDSSAQLMK MNDELRLKER QLEQIQLELT KANEKAVQLQ
970 980 990 1000 1010 1020
KNVEQTAQKA EQSQQETLKT HQEELKKMQD QLTDMKKQME TSQNQYKDLQ AKYEKETSEM
1030 1040 1050 1060 1070 1080
ITKHDADIKG FKQNLLDAEE ALKAAQKKND ELETQAEELK KQAEQAKADK RAEEVLQTME
1090 1100 1110 1120 1130 1140
KVTKEKDAIH QEKIETLASL ENSRQTNEKL QNELDMLKQN NLKNEEELTK SKELLNLENK
1150 1160 1170 1180 1190 1200
KVEELKKEFE ALKLAAAQKS QQLAALQEEN VKLAEELGRS RDEVTSHQKL EEERSVLNNQ
1210 1220 1230 1240 1250 1260
LLEMKKREST LKKEIDEERA SLQKSISDTS ALITQKDEEL EKLRNEITVL RGENASAKTL
1270 1280 1290 1300 1310 1320
QSVVKTLESD KLKLEEKVKN LEQKLKAKSE QPLTVTSPSG DIAANLLQDE SAEDKQQEID
1330 1340 1350 1360 1370 1380
FLNSVIVDLQ RRNEELNLKI QRMCEAALNG NEEETINYDS EEEGLSKKTP RLFCDICGCF
1390 1400 1410 1420 1430
DLHDTEDCPT QAQMLEEPPH STYHGSRREE RPYCDTCEMF GHWTADCNDD ETF