O35618

SS
Gene name
Mdm4 (Mdmx)
Protein name
Protein Mdm4
Names
Double minute 4 protein, Mdm2-like p53-binding protein, Protein Mdmx, p53-binding protein Mdm4
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:17248
EC number
Protein Class

Descriptions

MDM4 is a homologous protein that binds to p53 and regulates its activity. Intrinsically disordered region constitute an inhibitory module. It competes with the p53TAD for binding to the NTD of MDM4, thus lowering the affinity of MDMX for p53. Efficient binding of MDM4 to p53 requires a mechanism to relieve the allosteric self-inhibition.

Autoinhibitory domains (AIDs)

190-210 (Tryptohpan-rich segment) SS

Target domain

25-108 (SWIB/MDM2 domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for O35618

Entry ID Method Resolution Chain Position Source
AF-O35618-F1 Predicted AlphaFoldDB

7 variants for O35618

Variant ID(s) Position Change Description Diseaes Association Provenance
rs6192152 112 I>N No Ensembl
rs253523478 210 C>Y No Ensembl
rs582516334 274 T>M No Ensembl
rs245555288 401 E>G No Ensembl
rs243387396 409 A>E No Ensembl
rs218592325 411 E>G No Ensembl
rs223796411 475 A>V No Ensembl

No associated diseases with O35618

3 regional properties for O35618

Type Name Position InterPro Accession
domain Zinc finger, RING-type 436 - 477 IPR001841
domain Zinc finger, RanBP2-type 299 - 328 IPR001876
domain SWIB/MDM2 domain 25 - 108 IPR003121

Functions

Description
EC Number
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

3 GO annotations of molecular function

Name Definition
enzyme binding Binding to an enzyme, a protein with catalytic activity.
metal ion binding Binding to a metal ion.
p53 binding Binding to one of the p53 family of proteins.

14 GO annotations of biological process

Name Definition
atrial septum development The progression of the atrial septum over time, from its initial formation to the mature structure.
atrioventricular valve morphogenesis The process in which the structure of the atrioventricular valve is generated and organized.
DNA damage response, signal transduction by p53 class mediator A cascade of processes induced by the cell cycle regulator phosphoprotein p53, or an equivalent protein, in response to the detection of DNA damage.
endocardial cushion morphogenesis The process in which the anatomical structure of the endocardial cushion is generated and organized. The endocardial cushion is a specialized region of mesenchymal cells that will give rise to the heart septa and valves.
heart valve development The progression of a heart valve over time, from its formation to the mature structure. A heart valve is a structure that restricts the flow of blood to different regions of the heart and forms from an endocardial cushion.
negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator Any process that stops, prevents or reduces the frequency, rate or extent of intrinsic apoptotic signaling pathway by p53 class mediator.
negative regulation of protein catabolic process Any process that stops, prevents or reduces the frequency, rate or extent of protein catabolic process.
negative regulation of transcription by RNA polymerase II Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II.
positive regulation of cell population proliferation Any process that activates or increases the rate or extent of cell proliferation.
protein-containing complex assembly The aggregation, arrangement and bonding together of a set of macromolecules to form a protein-containing complex.
regulation of cell cycle Any process that modulates the rate or extent of progression through the cell cycle.
regulation of heart rate Any process that modulates the frequency or rate of heart contraction.
regulation of transcription by RNA polymerase II Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
ventricular septum development The progression of the ventricular septum over time from its formation to the mature structure.

6 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q2HJ21 MDM4 Protein Mdm4 Bos taurus (Bovine) SS
E1C4B0 MDM4 Double minute 4 protein Gallus gallus (Chicken) SS
O15151 MDM4 Protein Mdm4 Homo sapiens (Human) EV
Q8VCM5 Mul1 Mitochondrial ubiquitin ligase activator of NFKB 1 Mus musculus (Mouse) PR
Q5XIN1 Mdm4 Protein Mdm4 Rattus norvegicus (Rat) SS
Q7ZUW7 mdm4 Protein Mdm4 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MTSHSTSAQC SASDSACRIS SEQISQVRPK LQLLKILHAA GAQGEVFTMK EVMHYLGQYI
70 80 90 100 110 120
MVKQLYDQQE QHMVYCGGDL LGDLLGCQSF SVKDPSPLYD MLRKNLVTSA SINTDAAQTL
130 140 150 160 170 180
ALAQDHTMDF PSQDRLKHGA TEYSNPRKRT EEEDTHTLPT SRHKCRDSRA DEDLIEHLSQ
190 200 210 220 230 240
DETSRLDLDF EEWDVAGLPW WFLGNLRNNC IPKSNGSTDL QTNQDIGTAI VSDTTDDLWF
250 260 270 280 290 300
LNETVSEQLG VGIKVEAANS EQTSEVGKTS NKKTVEVGKD DDLEDSRSLS DDTDVELTSE
310 320 330 340 350 360
DEWQCTECKK FNSPSKRYCF RCWALRKDWY SDCSKLTHSL STSNITAIPE KKDNEGIDVP
370 380 390 400 410 420
DCRRTISAPV VRPKDGYLKE EKPRFDPCNS VGFLDLAHSS ESQEIISSAR EQTDIFSEQK
430 440 450 460 470 480
AETESMEDFQ NVLKPCSLCE KRPRDGNIIH GKTSHLTTCF HCARRLKKSG ASCPACKKEI
QLVIKVFIA