O16810
Gene name |
Orc1 (CG10667) |
Protein name |
Origin recognition complex subunit 1 |
Names |
DmORC1 |
Species |
Drosophila melanogaster (Fruit fly) |
KEGG Pathway |
dme:Dmel_CG10667 |
EC number |
|
Protein Class |
CELL DIVISION CONTROL PROTEIN 6-RELATED (PTHR10763) |
Descriptions
The eukaryotic origin recognition complex (ORC) binds replication origins and assembles the pre-replication complex necessary to initiate DNA replication. Replication initiation is initiated by the six-subunit ORC assembly: five of the six subunits of ORC (Orc1-5) retain AAA+ modules, while the sixth (Orc6) is composed of tandem cyclin-box folds similar to TFIIB. During initiation, ORC binds replication origins, recruiting another AAA1 ATPase, Cdc6, to DNA in a nucleotide-dependent manner. The DNA-bound ORC-Cdc6 complex in turn recruits the MCM2–7 replicative helicase and its associated Cdt1 chaperone to origins, promoting the loading of MCM2–7 complexes onto DNA.
Drosophila ORC complex can switch between active and autoinhibited conformations, providing a novel means for cell cycle and/or developmental control of ORC functions. This switch involves out-of-plane rotation of the Orc1 ATPase domain disrupting interactions and sealing off the central channel.
Autoinhibitory domains (AIDs)
Target domain |
456-187 (BAH domain) |
Relief mechanism |
PTM |
Assay |
Structural analysis |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
9 structures for O16810
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 4XGC | X-ray | 350 A | A | 533-924 | PDB |
| 7JGR | EM | 390 A | A | 440-924 | PDB |
| 7JGS | EM | 320 A | A | 440-924 | PDB |
| 7JK2 | EM | 320 A | A | 440-924 | PDB |
| 7JK3 | EM | 340 A | A | 440-924 | PDB |
| 7JK4 | EM | 340 A | A | 440-924 | PDB |
| 7JK5 | EM | 390 A | A | 440-924 | PDB |
| 7JK6 | EM | 400 A | A | 440-924 | PDB |
| AF-O16810-F1 | Predicted | AlphaFoldDB |
No variants for O16810
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for O16810 | |||||
No associated diseases with O16810
5 regional properties for O16810
Functions
| Description | ||
|---|---|---|
| EC Number | ||
| Subcellular Localization |
|
|
| PANTHER Family | PTHR10763 | CELL DIVISION CONTROL PROTEIN 6-RELATED |
| PANTHER Subfamily | PTHR10763:SF23 | ORIGIN RECOGNITION COMPLEX SUBUNIT 1 |
| PANTHER Protein Class |
DNA metabolism protein
replication origin binding protein |
|
| PANTHER Pathway Category | No pathway information available | |
2 GO annotations of cellular component
| Name | Definition |
|---|---|
| nuclear origin of replication recognition complex | A multisubunit complex that is located at the replication origins of a chromosome in the nucleus. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ATP hydrolysis activity | Catalysis of the reaction |
| chromatin binding | Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase. |
| DNA binding | Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). |
| metal ion binding | Binding to a metal ion. |
3 GO annotations of biological process
| Name | Definition |
|---|---|
| DNA amplification | The process in which the number of copies of a gene is increased in certain cells as extra copies of DNA are made in response to certain signals of cell development or of stress from the environment. |
| DNA replication initiation | The process in which DNA-dependent DNA replication is started; it begins when specific sequences, known as origins of replication, are recognized and bound by the origin recognition complex, followed by DNA unwinding. |
| mitotic DNA replication checkpoint signaling | A signal transduction process that contributes to a mitotic DNA replication checkpoint. |
7 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P54784 | ORC1 | Origin recognition complex subunit 1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q58DC8 | ORC1 | Origin recognition complex subunit 1 | Bos taurus (Bovine) | PR |
| Q13415 | ORC1 | Origin recognition complex subunit 1 | Homo sapiens (Human) | PR |
| Q9Z1N2 | Orc1 | Origin recognition complex subunit 1 | Mus musculus (Mouse) | PR |
| Q80Z32 | Orc1 | Origin recognition complex subunit 1 | Rattus norvegicus (Rat) | PR |
| Q710E8 | ORC1A | Origin of replication complex subunit 1A | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SU24 | ORC1B | Origin of replication complex subunit 1B | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MVNKENARSV | GQQVKWIGSQ | DELPPVKNLE | HKNVYFYQKC | IYGPLTLSVG | DFILVSNADA |
| 70 | 80 | 90 | 100 | 110 | 120 |
| AEPDTVSGCD | VARILHMYEL | RELTDREPCR | AIVQWYSWPK | AIPHNKYDDD | EVAIDFSLEV |
| 130 | 140 | 150 | 160 | 170 | 180 |
| IEEHRPYDND | VALGAIYRKC | IVLEGTSKTS | AEEILKRHAN | KLKSTACPMF | VSRYRFVKVK |
| 190 | 200 | 210 | 220 | 230 | 240 |
| RSYRLIPLEI | HLEQPEDNAR | PTRSSRKSLT | AHRESKRSIS | ARHDDTAGNK | GSSVEKRRRA |
| 250 | 260 | 270 | 280 | 290 | 300 |
| SMAASSSVEF | IDVNSFICEN | KVSPIKIVGG | RSVVRLSEKK | NAPEINANYL | PASPLTEKNA |
| 310 | 320 | 330 | 340 | 350 | 360 |
| KVETPKSRAS | AARRNLNLSL | DRGADTTADS | DCLNYSIVQQ | TPDPKTPSND | MKIKLRLSER |
| 370 | 380 | 390 | 400 | 410 | 420 |
| RRSVRLASMD | VDPLSLEEAV | QEPNAQGRKR | LGVANGDIYH | TPTKKSKEPL | ESAAATEQTP |
| 430 | 440 | 450 | 460 | 470 | 480 |
| STRRKSILKS | ATSRLAEGTP | RRSIHLSNIV | EQRVFEDDEI | ISTPKRGRSK | KTVQDNDEDY |
| 490 | 500 | 510 | 520 | 530 | 540 |
| SPKKSVQKTP | TRTRRSSTTT | KTATTPSKGI | TTATATPMTP | SQKMKKIRAG | ELSPSMQQRT |
| 550 | 560 | 570 | 580 | 590 | 600 |
| DLPAKDSSKS | ELQLAREQLH | VSVVPKSLPC | REREFENIYA | FLEGKIQDQC | GGCMYVSGVP |
| 610 | 620 | 630 | 640 | 650 | 660 |
| GTGKTATVTG | VIRTLQRMAK | QNELPAFEYL | EINGMRLTEP | RQAYVQIYKQ | LTGKTVSWEQ |
| 670 | 680 | 690 | 700 | 710 | 720 |
| AHALLEKRFT | TPAPRRVTTV | LLVDELDILC | NRRQDVVYNL | LDWPTKSAAK | LVVVTIANTM |
| 730 | 740 | 750 | 760 | 770 | 780 |
| DLPERLLMGK | VTSRLGLTRL | TFQPYSHKQL | QEIVTARLGG | SETFKGEAVQ | LVARKVAAVS |
| 790 | 800 | 810 | 820 | 830 | 840 |
| GDARRALDIC | RRATEIADTA | AVKCVTMLHV | QQALAEMIAS | AKVQAIRNCS | RMEQIFLQAI |
| 850 | 860 | 870 | 880 | 890 | 900 |
| AAEVTRTGVE | ETTFMGVYQQ | VETIAAFMGV | TFPPPGRALR | LCSKLGAERL | IISEHSRNDL |
| 910 | 920 | ||||
| FQKILLNVSA | DDIHYALRVE | EMVN |