O13154
SS
Gene name |
PACSIN2 |
Protein name |
Protein kinase C and casein kinase substrate in neurons protein 2 |
Names |
Focal adhesion protein of 52 kDa, FAP52 |
Species |
Gallus gallus (Chicken) |
KEGG Pathway |
gga:395975 |
EC number |
|
Protein Class |
|
Descriptions
PACSINs plays a central role in synaptic vesicle recycling and receptor-mediated endocytosis and controlling the cell surface expression of transmembrane proteins. The C-terminal SH3 of human homologous PACSIN2 (Q9UNF0) is an autoinhibitory domain affecting activation of the F-BAR domain. The competing binding of protein ligands to the SH3 or linker domain or posttranslational modifications of PACSIN2 permits activation of the F-BAR domain.
Autoinhibitory domains (AIDs)
Target domain |
11-282 (F-BAR domain) |
Relief mechanism |
Ligand binding |
Assay |
|
Accessory elements
No accessory elements
References
- Shimada A et al. (2010) "Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II", FEBS letters, 584, 1111-8
- Senju Y et al. (2011) "Essential role of PACSIN2/syndapin-II in caveolae membrane sculpting", Journal of cell science, 124, 2032-40
- Wang Q et al. (2009) "Molecular mechanism of membrane constriction and tubulation mediated by the F-BAR protein Pacsin/Syndapin", Proceedings of the National Academy of Sciences of the United States of America, 106, 12700-5
- Rao Y et al. (2010) "Molecular basis for SH3 domain regulation of F-BAR-mediated membrane deformation", Proceedings of the National Academy of Sciences of the United States of America, 107, 8213-8
- Goh SL et al. (2012) "Versatile membrane deformation potential of activated pacsin", PloS one, 7, e51628
Autoinhibited structure
Activated structure
2 structures for O13154
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 4BNE | X-ray | 257 A | A/B | 1-448 | PDB |
| AF-O13154-F1 | Predicted | AlphaFoldDB |
No variants for O13154
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for O13154 | |||||
No associated diseases with O13154
Functions
10 GO annotations of cellular component
| Name | Definition |
|---|---|
| caveola | A membrane raft that forms small pit, depression, or invagination that communicates with the outside of a cell and extends inward, indenting the cytoplasm and the cell membrane. Examples include flask-shaped invaginations of the plasma membrane in adipocytes associated with caveolin proteins, and minute pits or incuppings of the cell membrane formed during pinocytosis. Caveolae may be pinched off to form free vesicles within the cytoplasm. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytoskeleton | A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| early endosome | A membrane-bounded organelle that receives incoming material from primary endocytic vesicles that have been generated by clathrin-dependent and clathrin-independent endocytosis; vesicles fuse with the early endosome to deliver cargo for sorting into recycling or degradation pathways. |
| endosome | A vacuole to which materials ingested by endocytosis are delivered. |
| focal adhesion | A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ). |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| recycling endosome membrane | The lipid bilayer surrounding a recycling endosome. |
| ruffle membrane | The portion of the plasma membrane surrounding a ruffle. |
3 GO annotations of molecular function
| Name | Definition |
|---|---|
| cytoskeletal protein binding | Binding to a protein component of a cytoskeleton (actin, microtubule, or intermediate filament cytoskeleton). |
| phosphatidic acid binding | Binding to phosphatidic acid, any of a class of glycerol phosphate in which both the remaining hydroxyl groups of the glycerol moiety are esterified with fatty acids. |
| phospholipid binding | Binding to a phospholipid, a class of lipids containing phosphoric acid as a mono- or diester. |
7 GO annotations of biological process
| Name | Definition |
|---|---|
| actin cytoskeleton organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins. |
| caveola assembly | The aggregation, arrangement and bonding together of a set of components to form a caveola. A caveola is a plasma membrane raft that forms a small pit, depression, or invagination that communicates with the outside of a cell and extends inward, indenting the cytoplasm and the cell membrane. |
| cytoskeleton organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures. |
| endocytosis | A vesicle-mediated transport process in which cells take up external materials or membrane constituents by the invagination of a small region of the plasma membrane to form a new membrane-bounded vesicle. |
| negative regulation of endocytosis | Any process that stops, prevents, or reduces the frequency, rate or extent of endocytosis. |
| plasma membrane tubulation | A membrane tubulation process occurring in a plasma membrane. |
| regulation of endocytosis | Any process that modulates the frequency, rate or extent of endocytosis. |
11 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P25623 | SYP1 | Suppressor of yeast profilin deletion | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| A7MBI0 | PACSIN1 | Protein kinase C and casein kinase substrate in neurons protein 1 | Bos taurus (Bovine) | SS |
| Q9UKS6 | PACSIN3 | Protein kinase C and casein kinase substrate in neurons protein 3 | Homo sapiens (Human) | SS |
| Q9BY11 | PACSIN1 | Protein kinase C and casein kinase substrate in neurons protein 1 | Homo sapiens (Human) | EV |
| Q9UNF0 | PACSIN2 | Protein kinase C and casein kinase substrate in neurons protein 2 | Homo sapiens (Human) | EV |
| Q61644 | Pacsin1 | Protein kinase C and casein kinase substrate in neurons protein 1 | Mus musculus (Mouse) | EV |
| Q99JB8 | Pacsin3 | Protein kinase C and casein kinase II substrate protein 3 | Mus musculus (Mouse) | SS |
| Q9WVE8 | Pacsin2 | Protein kinase C and casein kinase substrate in neurons protein 2 | Mus musculus (Mouse) | EV |
| Q9Z0W5 | Pacsin1 | Protein kinase C and casein kinase substrate in neurons protein 1 | Rattus norvegicus (Rat) | SS |
| Q9QY17 | Pacsin2 | Protein kinase C and casein kinase substrate in neurons 2 protein | Rattus norvegicus (Rat) | SS |
| Q4V920 | pacsin1b | Protein kinase C and casein kinase substrate in neurons protein 1 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSGSYDDSVG | VEVSSDSFWE | VGNYKRTVKR | IDDGHRLCND | LMNCIHERAR | IEKVYAQQLT |
| 70 | 80 | 90 | 100 | 110 | 120 |
| EWAKRWKQLV | EKGPQYGTVE | RAWCAFMSEA | EKVSELHLEV | KGSLMNEDFE | KIKNWQKEAF |
| 130 | 140 | 150 | 160 | 170 | 180 |
| HKQMMGGFKE | TKEAEDGFRK | AQKPWAKKLK | EVEAAKKAYH | AACKEEKLAI | SRETNSKADP |
| 190 | 200 | 210 | 220 | 230 | 240 |
| ALNPEQLKKL | QDKVERSKQD | VLKTKAKYEK | SLKELDNATP | QYMENMEQVF | EQCQQFEEKR |
| 250 | 260 | 270 | 280 | 290 | 300 |
| LRFFREVLLE | VQKHLDLSNV | ASYKNIYREL | EQNIKTADAV | EDLRWFRANQ | GPGMSMNWPQ |
| 310 | 320 | 330 | 340 | 350 | 360 |
| FEDDEWSADL | NRTLSRREKK | KASDGVTLTG | INQTGDQVSQ | PNKHSSVSSY | EKNQSYPTDW |
| 370 | 380 | 390 | 400 | 410 | 420 |
| SDEESNNPFS | STDAKGDTNP | FDEDTSPVME | VRVRALYDYE | GQEQDELSFK | AGDELTKMEN |
| 430 | 440 | ||||
| EDEQGWCKGR | LDNGQVGLYP | ANYVEPIQ |