O13147

SS
Gene name
ephb3 (ek3, zek3)
Protein name
Ephrin type-B receptor 3
Names
EC 2.7.10.1 , EPH-like kinase 3 , Tyrosine-protein kinase receptor ZEK3
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
KEGG Pathway
EC number
2.7.10.1: Protein-tyrosine kinases
Protein Class

Descriptions

Ephrin type-B receptor 2 is a membrane-associated protein that mediates axon guidance, cell migration and morphogenesis. The Eph receptor tyrosine kinase family is regulated by autophosphorylation within the juxtamembrane region and the kinase activation segment. The structure, supported by mutagenesis data, reveals that the juxtamembrane segment adopts a helical conformation that distorts the small lobe of the kinase domain, and blocks the activation segment from attaining an activated conformation. Phosphorylation of the conserved juxtamembrane tyrosines would relieve this autoinhibition by disturbing the association of the juxtamembrane segment with the kinase domain, while liberating phosphotyrosine sites for binding SH2 domains of target proteins.

Autoinhibitory domains (AIDs)

161-187 (Juxtamembrane domain) SS

Target domain

187-450 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

329-357 (Activation loop from InterPro)

Target domain

187-450 (Protein kinase domain)

Relief mechanism

Assay

329-357 (Activation loop from InterPro)

Target domain

187-450 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for O13147

Entry ID Method Resolution Chain Position Source
AF-O13147-F1 Predicted AlphaFoldDB

No variants for O13147

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O13147

No associated diseases with O13147

8 regional properties for O13147

Type Name Position InterPro Accession
domain Protein kinase domain 187 - 450 IPR000719
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 187 - 446 IPR001245
domain Sterile alpha motif domain 429 - 491 IPR001660
domain Fibronectin type III 1 - 64 IPR003961
active_site Tyrosine-protein kinase, active site 308 - 320 IPR008266
binding_site Protein kinase, ATP binding site 193 - 219 IPR017441
domain Tyrosine-protein kinase, catalytic domain 187 - 446 IPR020635
domain Ephrin receptor, transmembrane domain 114 - 183 IPR027936

Functions

Description
EC Number 2.7.10.1 Protein-tyrosine kinases
Subcellular Localization
  • Cell membrane ; Single-pass type I membrane protein
  • Cell projection, dendrite
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
dendrite A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
receptor complex Any protein complex that undergoes combination with a hormone, neurotransmitter, drug or intracellular messenger to initiate a change in cell function.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ephrin receptor activity Combining with an ephrin receptor ligand to initiate a change in cell activity.
transmembrane receptor protein tyrosine kinase activity Combining with a signal and transmitting the signal from one side of the membrane to the other to initiate a change in cell activity by catalysis of the reaction
transmembrane-ephrin receptor activity Combining with a transmembrane ephrin to initiate a change in cell activity.

13 GO annotations of biological process

Name Definition
angiogenesis Blood vessel formation when new vessels emerge from the proliferation of pre-existing blood vessels.
axon guidance The chemotaxis process that directs the migration of an axon growth cone to a specific target site in response to a combination of attractive and repulsive cues.
axonal fasciculation The collection of axons into a bundle of rods, known as a fascicle.
cell migration The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues.
dendritic spine development The process whose specific outcome is the progression of the dendritic spine over time, from its formation to the mature structure. A dendritic spine is a protrusion from a dendrite and a specialized subcellular compartment involved in synaptic transmission.
dendritic spine morphogenesis The process in which the anatomical structures of a dendritic spine are generated and organized. A dendritic spine is a protrusion from a dendrite and a specialized subcellular compartment involved in synaptic transmission.
ephrin receptor signaling pathway The series of molecular signals initiated by ephrin binding to its receptor, and ending with the regulation of a downstream cellular process, e.g. transcription.
positive regulation of synapse assembly Any process that activates, maintains or increases the frequency, rate or extent of synapse assembly, the aggregation, arrangement and bonding together of a set of components to form a synapse.
protein autophosphorylation The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation).
regulation of axonogenesis Any process that modulates the frequency, rate or extent of axonogenesis, the generation of an axon, the long process of a neuron.
regulation of cell-cell adhesion Any process that modulates the frequency, rate or extent of attachment of a cell to another cell.
regulation of GTPase activity Any process that modulates the rate of GTP hydrolysis by a GTPase.
substrate adhesion-dependent cell spreading The morphogenetic process that results in flattening of a cell as a consequence of its adhesion to a substrate.

62 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P28693 EPHB2 Ephrin type-B receptor 2 Gallus gallus (Chicken) PR
Q07496 EPHA4 Ephrin type-A receptor 4 Gallus gallus (Chicken) SS
Q07494 EPHB1 Ephrin type-B receptor 1 Gallus gallus (Chicken) SS
Q07497 EPHB5 Ephrin type-B receptor 5 Gallus gallus (Chicken) PR
P29318 EPHA3 Ephrin type-A receptor 3 Gallus gallus (Chicken) SS
O42422 EPHA7 Ephrin type-A receptor 7 Gallus gallus (Chicken) SS
P54755 EPHA5 Ephrin type-A receptor 5 Gallus gallus (Chicken) SS
Q07498 EPHB3 Ephrin type-B receptor 3 Gallus gallus (Chicken) SS
P0C0K6 EPHB6 Ephrin type-B receptor 6 Pan troglodytes (Chimpanzee) SS
P29322 EPHA8 Ephrin type-A receptor 8 Homo sapiens (Human) SS
P21709 EPHA1 Ephrin type-A receptor 1 Homo sapiens (Human) SS
P54764 EPHA4 Ephrin type-A receptor 4 Homo sapiens (Human) SS
P29320 EPHA3 Ephrin type-A receptor 3 Homo sapiens (Human) PR
P54760 EPHB4 Ephrin type-B receptor 4 Homo sapiens (Human) SS
P29317 EPHA2 Ephrin type-A receptor 2 Homo sapiens (Human) SS
Q15375 EPHA7 Ephrin type-A receptor 7 Homo sapiens (Human) SS
Q5JZY3 EPHA10 Ephrin type-A receptor 10 Homo sapiens (Human) SS
P29323 EPHB2 Ephrin type-B receptor 2 Homo sapiens (Human) EV
Q9UF33 EPHA6 Ephrin type-A receptor 6 Homo sapiens (Human) SS
P54762 EPHB1 Ephrin type-B receptor 1 Homo sapiens (Human) SS
P54756 EPHA5 Ephrin type-A receptor 5 Homo sapiens (Human) SS
O15197 EPHB6 Ephrin type-B receptor 6 Homo sapiens (Human) SS
P54753 EPHB3 Ephrin type-B receptor 3 Homo sapiens (Human) SS
Q03145 Epha2 Ephrin type-A receptor 2 Mus musculus (Mouse) PR
Q03137 Epha4 Ephrin type-A receptor 4 Mus musculus (Mouse) SS
Q61772 Epha7 Ephrin type-A receptor 7 Mus musculus (Mouse) SS
O09127 Epha8 Ephrin type-A receptor 8 Mus musculus (Mouse) SS
Q8CBF3 Ephb1 Ephrin type-B receptor 1 Mus musculus (Mouse) SS
Q8BYG9 Epha10 Ephrin type-A receptor 10 Mus musculus (Mouse) SS
Q60629 Epha5 Ephrin type-A receptor 5 Mus musculus (Mouse) SS
P29319 Epha3 Ephrin type-A receptor 3 Mus musculus (Mouse) SS
Q62413 Epha6 Ephrin type-A receptor 6 Mus musculus (Mouse) SS
Q60750 Epha1 Ephrin type-A receptor 1 Mus musculus (Mouse) SS
P54763 Ephb2 Ephrin type-B receptor 2 Mus musculus (Mouse) SS
P54761 Ephb4 Ephrin type-B receptor 4 Mus musculus (Mouse) PR
O08644 Ephb6 Ephrin type-B receptor 6 Mus musculus (Mouse) PR
P54754 Ephb3 Ephrin type-B receptor 3 Mus musculus (Mouse) SS
P54757 Epha5 Ephrin type-A receptor 5 Rattus norvegicus (Rat) SS
P54759 Epha7 Ephrin type-A receptor 7 Rattus norvegicus (Rat) SS
P09759 Ephb1 Ephrin type-B receptor 1 Rattus norvegicus (Rat) SS
P0C0K7 Ephb6 Ephrin type-B receptor 6 Rattus norvegicus (Rat) SS
O08680 Epha3 Ephrin type-A receptor 3 Rattus norvegicus (Rat) SS
O61460 vab-1 Ephrin receptor 1 Caenorhabditis elegans SS
O22558 STY8 Serine/threonine-protein kinase STY8 Arabidopsis thaliana (Mouse-ear cress) PR
Q8RWL6 STY17 Serine/threonine-protein kinase STY17 Arabidopsis thaliana (Mouse-ear cress) PR
F4JTP5 STY46 Serine/threonine-protein kinase STY46 Arabidopsis thaliana (Mouse-ear cress) PR
O73878 ephb4b Ephrin type-B receptor 4b Danio rerio (Zebrafish) (Brachydanio rerio) SS
O13146 epha3 Ephrin type-A receptor 3 Danio rerio (Zebrafish) (Brachydanio rerio) SS
F1QVU0 ltk Tyrosine-protein kinase receptor Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q9YI66 tyro3 Tyrosine-protein kinase receptor TYRO3 Danio rerio (Zebrafish) (Brachydanio rerio) PR
E7F3X9 ddr1 receptor protein-tyrosine kinase Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q5MD89 flt4 Vascular endothelial growth factor receptor 3 Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q8AXB3 kdrl Vascular endothelial growth factor receptor kdr-like Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q5GIT4 kdr Vascular endothelial growth factor receptor 2 Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q90Z00 fgfr1a Fibroblast growth factor receptor 1-A Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q90413 fgfr4 Fibroblast growth factor receptor 4 Danio rerio (Zebrafish) (Brachydanio rerio) SS
O73791 tek Angiopoietin-1 receptor Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q8JFR5 kita Mast/stem cell growth factor receptor kita Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q9I8N6 csf1r Macrophage colony-stimulating factor 1 receptor Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q9DE49 pdgfra Platelet-derived growth factor receptor alpha Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q8JG38 fgfr2 Fibroblast growth factor receptor 2 Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q9I8X3 fgfr3 Fibroblast growth factor receptor 3 Danio rerio (Zebrafish) (Brachydanio rerio) PR
10 20 30 40 50 60
PLLVLDLIIQ ERGESFSHTV TAQHTSAKVE GLKAGTVYSV QVRARTVAGY GRYSNPVDFS
70 80 90 100 110 120
TSLYVCPVSS SSTSMHLRRR EELTTTTTGL KSREERFQKS DDPERSVQDL LPLIVGSASA
130 140 150 160 170 180
GFVVILAMIV IAVVCLRRQR TGSELEYTEK LQQYVSPGVK VYIDPFTYED PNEAVHEFAR
190 200 210 220 230 240
EIDISCVKIE EVIGAGEFGE VCRGRLKQAG RKETTVAIKT LKAGYTEHQR RDFLSEASIM
250 260 270 280 290 300
GQFDHPNVIH LEGVLTRSCP VLIVTEFMEN GALDSFLRLN DGRFTVTQLV GMLRGIAAGM
310 320 330 340 350 360
KYLSDMNYVH RDLAARNVLV NSNLVCKVSD FGLSRFLDDN SSDPTYTSSL GGKIPIRWTA
370 380 390 400 410 420
PEAIAFRKFT SASDVWSYGI VMWEVMSFGE RPYWDMSNQD VMNAVEQDYR LPPPMDCPAV
430 440 450 460 470 480
LHQLMLECWV KERNMRPRFG QIVSTLDKFL RNAASLKVLT STHSGDLCRI GGTLPGHQRK
490
SIGDAQDIKQ QMSQTLPIRV