O08911

SS
Gene name
Mapk12 (Sapk3)
Protein name
Mitogen-activated protein kinase 12
Names
MAP kinase 12 , MAPK 12 , EC 2.7.11.24 , Extracellular signal-regulated kinase 6 , ERK-6 , Mitogen-activated protein kinase p38 gamma , MAP kinase p38 gamma , Stress-activated protein kinase 3
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:29857
EC number
2.7.11.24: Protein-serine/threonine kinases
Protein Class

Descriptions

MAPK1 encodes Mitogen-activated protein kinase 1, and is involved in diverse cellular responses by phosphorylating cytoplasmic and nuclear proteins. Unlike other MAP kinases, MAPK1 activates via dual phosphorylation of Thr-186 and Tyr-193 within the activation lip, and mutations at Q105 alone were sufficient for autoactivation. Additionally, in the inactive form, L76, Q10 and I87 form a structural unit that restrains intramolecular autophosphorylation by preventing interactions between activation lip residues and the catalytic base (D149) needed for phosphoryl transfer. Mutations at L76, I87 and additionally S153 increased basal specific activity via autophosphorylation of Thr-186 and Tyr-193.

Autoinhibitory domains (AIDs)

76-155 (Autoinhibitory region) SS

Target domain

27-311 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

170-190 (Activation loop from InterPro)

Target domain

27-311 (Protein kinase domain)

Relief mechanism

Assay

170-190 (Activation loop from InterPro)

Target domain

27-311 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for O08911

Entry ID Method Resolution Chain Position Source
AF-O08911-F1 Predicted AlphaFoldDB

24 variants for O08911

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3389372708 3 S>F No EVA
rs3389385890 25 A>D No EVA
rs3389344881 35 S>P No EVA
rs3389379867 44 A>E No EVA
rs3389351240 50 G>V No EVA
rs3389332739 77 L>I No EVA
rs3389376507 107 L>Q No EVA
rs3389300109 121 K>E No EVA
rs3389344910 126 S>I No EVA
rs1132267248 154 L>F No EVA
rs3389372680 163 E>K No EVA
rs3389377537 195 E>K No EVA
rs3389375400 201 M>K No EVA
rs3413051417 247 P>H No EVA
rs3389397362 267 E>D No EVA
rs3389397351 276 V>A No EVA
rs3389391351 278 T>I No EVA
rs3389385979 291 M>I No EVA
rs3389385943 297 E>G No EVA
rs3406128086 311 F>S No EVA
rs3406458153 312 E>V No EVA
rs3389375386 314 L>R No EVA
rs3389379810 328 D>H No EVA
rs3389382088 336 T>N No EVA

No associated diseases with O08911

3 regional properties for O08911

Type Name Position InterPro Accession
domain Protein kinase domain 27 - 311 IPR000719
conserved_site Mitogen-activated protein (MAP) kinase, conserved site 62 - 165 IPR003527
binding_site Protein kinase, ATP binding site 33 - 57 IPR017441

Functions

Description
EC Number 2.7.11.24 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Nucleus
  • Mitochondrion
  • Mitochondrial when associated with SH3BP5
  • In skeletal muscle colocalizes with SNTA1 at the neuromuscular junction and throughout the sarcolemma
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
magnesium ion binding Binding to a magnesium (Mg) ion.
MAP kinase activity Catalysis of the reaction
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

5 GO annotations of biological process

Name Definition
cell cycle The progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events. Canonically, the cell cycle comprises the replication and segregation of genetic material followed by the division of the cell, but in endocycles or syncytial cells nuclear replication or nuclear division may not be followed by cell division.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
myoblast differentiation The process in which a relatively unspecialized cell acquires specialized features of a myoblast. A myoblast is a mononucleate cell type that, by fusion with other myoblasts, gives rise to the myotubes that eventually develop into striated muscle fibers.
negative regulation of cell cycle Any process that stops, prevents or reduces the rate or extent of progression through the cell cycle.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

90 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P32485 HOG1 Mitogen-activated protein kinase HOG1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
A5PKJ4 MAPK7 Mitogen-activated protein kinase 7 Bos taurus (Bovine) SS
Q3T0N5 MAPK13 Mitogen-activated protein kinase 13 Bos taurus (Bovine) SS
P46196 MAPK1 Mitogen-activated protein kinase 1 Bos taurus (Bovine) SS
Q5F3W3 MAPK6 Mitogen-activated protein kinase 6 Gallus gallus (Chicken) SS
Q95NE7 MAPK14 Mitogen-activated protein kinase 14 Pan troglodytes (Chimpanzee) SS
Q9N272 MAPK13 Mitogen-activated protein kinase 13 Pan troglodytes (Chimpanzee) SS
Q9W354 Erk7 Extracellular signal-regulated kinase 7 Drosophila melanogaster (Fruit fly) SS
P40417 rl Mitogen-activated protein kinase ERK-A Drosophila melanogaster (Fruit fly) SS
O61443 p38b Mitogen-activated protein kinase p38b Drosophila melanogaster (Fruit fly) SS
O62618 p38a Mitogen-activated protein kinase p38a Drosophila melanogaster (Fruit fly) SS
P83100 p38c Putative mitogen-activated protein kinase 14C Drosophila melanogaster (Fruit fly) SS
O15264 MAPK13 Mitogen-activated protein kinase 13 Homo sapiens (Human) SS
P28482 MAPK1 Mitogen-activated protein kinase 1 Homo sapiens (Human) EV
Q16539 MAPK14 Mitogen-activated protein kinase 14 Homo sapiens (Human) SS
Q16659 MAPK6 Mitogen-activated protein kinase 6 Homo sapiens (Human) SS
P27361 MAPK3 Mitogen-activated protein kinase 3 Homo sapiens (Human) SS
Q13164 MAPK7 Mitogen-activated protein kinase 7 Homo sapiens (Human) SS
Q15759 MAPK11 Mitogen-activated protein kinase 11 Homo sapiens (Human) SS
Q8TD08 MAPK15 Mitogen-activated protein kinase 15 Homo sapiens (Human) SS
P31152 MAPK4 Mitogen-activated protein kinase 4 Homo sapiens (Human) SS
P53778 MAPK12 Mitogen-activated protein kinase 12 Homo sapiens (Human) SS
P63085 Mapk1 Mitogen-activated protein kinase 1 Mus musculus (Mouse) SS
Q61532 Mapk6 Mitogen-activated protein kinase 6 Mus musculus (Mouse) SS
Q63844 Mapk3 Mitogen-activated protein kinase 3 Mus musculus (Mouse) SS
Q6P5G0 Mapk4 Mitogen-activated protein kinase 4 Mus musculus (Mouse) SS
Q80Y86 Mapk15 Mitogen-activated protein kinase 15 Mus musculus (Mouse) SS
Q9WUI1 Mapk11 Mitogen-activated protein kinase 11 Mus musculus (Mouse) SS
Q9Z1B7 Mapk13 Mitogen-activated protein kinase 13 Mus musculus (Mouse) SS
Q9JKV2 Cilk1 Serine/threonine-protein kinase ICK Mus musculus (Mouse) PR
Q04859 Mak Serine/threonine-protein kinase MAK Mus musculus (Mouse) PR
Q9WVS4 Mok MAPK/MAK/MRK overlapping kinase Mus musculus (Mouse) PR
Q9WTU6 Mapk9 Mitogen-activated protein kinase 9 Mus musculus (Mouse) PR
Q91Y86 Mapk8 Mitogen-activated protein kinase 8 Mus musculus (Mouse) PR
Q9WVS8 Mapk7 Mitogen-activated protein kinase 7 Mus musculus (Mouse) SS
P47811 Mapk14 Mitogen-activated protein kinase 14 Mus musculus (Mouse) SS
P70618 Mapk14 Mitogen-activated protein kinase 14 Rattus norvegicus (Rat) SS
Q9WTY9 Mapk13 Mitogen-activated protein kinase 13 Rattus norvegicus (Rat) SS
P63086 Mapk1 Mitogen-activated protein kinase 1 Rattus norvegicus (Rat) SS
Q9Z2A6 Mapk15 Mitogen-activated protein kinase 15 Rattus norvegicus (Rat) SS
P27704 Mapk6 Mitogen-activated protein kinase 6 Rattus norvegicus (Rat) SS
P21708 Mapk3 Mitogen-activated protein kinase 3 Rattus norvegicus (Rat) SS
Q63454 Mapk4 Mitogen-activated protein kinase 4 Rattus norvegicus (Rat) SS
P0C865 Mapk7 Mitogen-activated protein kinase 7 Rattus norvegicus (Rat) SS
Q63538 Mapk12 Mitogen-activated protein kinase 12 Rattus norvegicus (Rat) SS
Q5ZCI1 MPK10 Mitogen-activated protein kinase 10 Oryza sativa subsp. japonica (Rice) SS
Q5VP69 MPK16 Mitogen-activated protein kinase 16 Oryza sativa subsp. japonica (Rice) SS
Q5SN53 MPK8 Mitogen-activated protein kinase 8 Oryza sativa subsp. japonica (Rice) SS
Q0E459 MPK13 Mitogen-activated protein kinase 13 Oryza sativa subsp. japonica (Rice) SS
Q6Z437 MPK3 Mitogen-activated protein kinase 3 Oryza sativa subsp. japonica (Rice) SS
Q75KK8 MPK14 Mitogen-activated protein kinase 14 Oryza sativa subsp. japonica (Rice) SS
Q67C40 MPK7 Mitogen-activated protein kinase 7 Oryza sativa subsp. japonica (Rice) SS
Q6L5F7 MPK17 Mitogen-activated protein kinase 17 Oryza sativa subsp. japonica (Rice) SS
Q5VN19 MPK11 Mitogen-activated protein kinase 11 Oryza sativa subsp. japonica (Rice) SS
Q5Z859 MPK4 Mitogen-activated protein kinase 4 Oryza sativa subsp. japonica (Rice) SS
Q5Z9J0 MPK12 Mitogen-activated protein kinase 12 Oryza sativa subsp. japonica (Rice) SS
Q53N72 MPK15 Mitogen-activated protein kinase 15 Oryza sativa subsp. japonica (Rice) SS
Q336X9 MPK6 Mitogen-activated protein kinase 6 Oryza sativa subsp. japonica (Rice) SS
Q5J4W4 MPK2 Mitogen-activated protein kinase 2 Oryza sativa subsp. japonica (Rice) SS
Q84UI5 MPK1 Mitogen-activated protein kinase 1 Oryza sativa subsp. japonica (Rice) SS
Q10N20 MPK5 Mitogen-activated protein kinase 5 Oryza sativa subsp. japonica (Rice) SS
Q11179 mapk-15 Mitogen-activated protein kinase 15 Caenorhabditis elegans SS
O44514 pmk-3 Mitogen-activated protein kinase pmk-3 Caenorhabditis elegans PR
P39745 mpk-1 Mitogen-activated protein kinase mpk-1 Caenorhabditis elegans SS
G5EBT1 sma-5 Mitogen-activated protein kinase sma-5 Caenorhabditis elegans SS
Q17446 pmk-1 Mitogen-activated protein kinase pmk-1 Caenorhabditis elegans SS
Q8MXI4 pmk-2 Mitogen-activated protein kinase pmk-2 Caenorhabditis elegans SS
Q39021 MPK1 Mitogen-activated protein kinase 1 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LM33 MPK8 Mitogen-activated protein kinase 8 Arabidopsis thaliana (Mouse-ear cress) SS
Q9C5C0 MPK18 Mitogen-activated protein kinase 18 Arabidopsis thaliana (Mouse-ear cress) SS
Q39022 MPK2 Mitogen-activated protein kinase 2 Arabidopsis thaliana (Mouse-ear cress) SS
Q9C9U4 MPK15 Mitogen-activated protein kinase 15 Arabidopsis thaliana (Mouse-ear cress) SS
Q84M93 MPK17 Mitogen-activated protein kinase 17 Arabidopsis thaliana (Mouse-ear cress) SS
Q39027 MPK7 Mitogen-activated protein kinase 7 Arabidopsis thaliana (Mouse-ear cress) SS
Q9SJG9 MPK20 Mitogen-activated protein kinase 20 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LUC3 MPK19 Mitogen-activated protein kinase 19 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LV37 MPK9 Mitogen-activated protein kinase 9 Arabidopsis thaliana (Mouse-ear cress) SS
O23236 MPK14 Mitogen-activated protein kinase 14 Arabidopsis thaliana (Mouse-ear cress) SS
Q8W4J2 MPK16 Mitogen-activated protein kinase 16 Arabidopsis thaliana (Mouse-ear cress) SS
Q39023 MPK3 Mitogen-activated protein kinase 3 Arabidopsis thaliana (Mouse-ear cress) SS
Q39024 MPK4 Mitogen-activated protein kinase 4 Arabidopsis thaliana (Mouse-ear cress) SS
Q39025 MPK5 Mitogen-activated protein kinase 5 Arabidopsis thaliana (Mouse-ear cress) SS
Q39026 MPK6 Mitogen-activated protein kinase 6 Arabidopsis thaliana (Mouse-ear cress) SS
Q9M1Z5 MPK10 Mitogen-activated protein kinase 10 Arabidopsis thaliana (Mouse-ear cress) SS
Q8GYQ5 MPK12 Mitogen-activated protein kinase 12 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LMM5 MPK11 Mitogen-activated protein kinase 11 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LQQ9 MPK13 Mitogen-activated protein kinase 13 Arabidopsis thaliana (Mouse-ear cress) SS
Q9DGE1 mapk14b Mitogen-activated protein kinase 14B Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q9DGE2 mapk14a Mitogen-activated protein kinase 14A Danio rerio (Zebrafish) (Brachydanio rerio) SS
O42376 mapk12 Mitogen-activated protein kinase 12 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MSSPPPARKG FYRQEVTKTA WEVRAVYQDL QPVGSGAYGA VCSAVDSRTG NKVAIKKLYR
70 80 90 100 110 120
PFQSELFAKR AYRELRLLKH MRHENVIGLL DVFTPDESLD DFTDFYLVMP FMGTDLGKLM
130 140 150 160 170 180
KHETLSEDRI QFLVYQMLKG LKYIHAAGVI HRDLKPGNLA VNEDCELKIL DFGLARQADS
190 200 210 220 230 240
EMTGYVVTRW YRAPEVILNW MRYTQTVDIW SVGCIMAEMI TGKILFKGND HLDQLKEIMK
250 260 270 280 290 300
ITGTPPPEFV QKLQSAEAKN YMEGLPELEK KDFASVLTNA SPQAVNLLER MLVLDAEQRV
310 320 330 340 350 360
TAAEALTHPY FESLRDTEDE PKAQKYDDSF DDVDRTLEEW KRVTYKEVLS FKPPRQLGAR
VPKETAL