AiPD: Autoinhibited Protein Database

O08595

Gene name	Sec22b (Ers-24)
Protein name	Vesicle-trafficking protein SEC22b
Names	ER-Golgi SNARE of 24 kDa, ERS-24, ERS24, SEC22 vesicle-trafficking protein homolog B
Species	Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
KEGG Pathway	cge:100689313
EC number
Protein Class

Descriptions

Sec22b is a SNARE involved in endoplasmic reticulum/Golgi membrane traffickingis. The N-terminal region of Sec22b regulates the function of SNARE. In rat SNARE Ykt6 (Q5EGY4), the SNARE core is autoinhibited by N-terminal longin domain.

Autoinhibitory domains (AIDs)

1-130 (N-terminal longin domain) SS

Target domain	134-194 (v-SNARE coiled-coil homology)
Relief mechanism	Others, PTM
Assay

AID

1-130 (N-terminal longin domain)

Target domain

134-194 (v-SNARE coiled-coil homology)

Relief mechanism

Others (self-oligomerization through SNARE domain), PTM (Removal of farnesyl group from the C-terminal tail, Palmitoylation of the C-terminal tail

Assay

Accessory elements

No accessory elements

References

Gonzalez LC Jr et al. (2001) "A novel snare N-terminal domain revealed by the crystal structure of Sec22b", The Journal of biological chemistry, 276, 24203-11

Wen W et al. (2010) "Lipid-Induced conformational switch controls fusion activity of longin domain SNARE Ykt6", Molecular cell, 37, 383-95

Autoinhibited structure

Activated structure

1 structures for O08595

Entry ID	Method	Resolution	Chain	Position	Source
AF-O08595-F1	Predicted				AlphaFoldDB

No variants for O08595

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
No variants for O08595

No associated diseases with O08595

2 regional properties for O08595

Type	Name	Position	InterPro Accession
domain	Ubiquitin-like domain	15 - 92	IPR000626
domain	Rad60/SUMO-like domain	17 - 85	IPR022617

Functions

		Description
EC Number
Subcellular Localization	Endoplasmic reticulum membrane ; Single-pass type IV membrane protein Endoplasmic reticulum-Golgi intermediate compartment membrane Golgi apparatus, cis-Golgi network membrane Golgi apparatus, trans-Golgi network membrane Melanosome	Concentrated most in the intermediate compartment/cis-Golgi network and the cis-Golgi cisternae 1 and 2 Greatly reduced in concentration at the trans end of the Golgi apparatus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

5 GO annotations of cellular component

Name	Definition
endoplasmic reticulum membrane	The lipid bilayer surrounding the endoplasmic reticulum.
endoplasmic reticulum-Golgi intermediate compartment membrane	The lipid bilayer surrounding any of the compartments of the endoplasmic reticulum (ER)-Golgi intermediate compartment system.
Golgi apparatus	A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
integral component of membrane	The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
melanosome	A tissue-specific, membrane-bounded cytoplasmic organelle within which melanin pigments are synthesized and stored. Melanosomes are synthesized in melanocyte cells.

1 GO annotations of molecular function

Name	Definition
SNAP receptor activity	Acting as a marker to identify a membrane and interacting selectively with one or more SNAREs on another membrane to mediate membrane fusion.

3 GO annotations of biological process

Name	Definition
endoplasmic reticulum to Golgi vesicle-mediated transport	The directed movement of substances from the endoplasmic reticulum (ER) to the Golgi, mediated by COP II vesicles. Small COP II coated vesicles form from the ER and then fuse directly with the cis-Golgi. Larger structures are transported along microtubules to the cis-Golgi.
protein transport	The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum	The directed movement of substances from the Golgi back to the endoplasmic reticulum, mediated by vesicles bearing specific protein coats such as COPI or COG.

No homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
No homologous proteins

10	20	30	40	50	60
MVLLTMIARV	ADGLPLAASM	QEDEQSGRDL	QQYQSQAKQL	FRKLNEQSPT	RCTLGAGAMT
70	80	90	100	110	120
FHYIIEQGVC	YLVLCEAAFP	KKLAFAYLED	LHSEFDEQHG	KKVPTVSRPY	SFIEFDTFIQ
130	140	150	160	170	180
KTKKLYIDSR	ARRNLGSINT	ELQDVQRIMV	ANIEEVLQRG	EALSALDSKA	NNLSSLSKKY
190	200	210
RQDAKYLNMR	STYAKLAAVA	VFFIMLIVYV	RFWWL