O08561

PR
Gene name
Pi4kb (Pik4cb)
Protein name
Phosphatidylinositol 4-kinase beta
Names
PI4K-beta, PI4Kbeta, PtdIns 4-kinase beta
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:81747
EC number
2.7.1.67: Phosphotransferases with an alcohol group as acceptor
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-157 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

689-711 (Activation loop from InterPro)

Target domain

529-816 (Catalytic domain of Type III Phosphoinositide 4-kinase beta)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for O08561

Entry ID Method Resolution Chain Position Source
AF-O08561-F1 Predicted AlphaFoldDB

No variants for O08561

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O08561

No associated diseases with O08561

4 regional properties for O08561

Type Name Position InterPro Accession
domain Phosphatidylinositol 3-/4-kinase, catalytic domain 535 - 814 IPR000403
domain Phosphoinositide 3-kinase, accessory (PIK) domain 61 - 242 IPR001263
conserved_site Phosphatidylinositol 3/4-kinase, conserved site 563 - 577 IPR018936-1
conserved_site Phosphatidylinositol 3/4-kinase, conserved site 656 - 676 IPR018936-2

Functions

Description
EC Number 2.7.1.67 Phosphotransferases with an alcohol group as acceptor
Subcellular Localization
  • Golgi apparatus
  • Endomembrane system
  • Mitochondrion outer membrane ; Peripheral membrane protein
  • Rough endoplasmic reticulum membrane ; Peripheral membrane protein
  • Golgi apparatus membrane
  • Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum
  • Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) on the Golgi complex
  • Recruited to the Golgi apparatus membrane by ACBD3, GGA2 is also involved in the recruitment
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

8 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
Golgi membrane The lipid bilayer surrounding any of the compartments of the Golgi apparatus.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
mitochondrial outer membrane The outer, i.e. cytoplasm-facing, lipid bilayer of the mitochondrial envelope.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
rough endoplasmic reticulum membrane The lipid bilayer surrounding the rough endoplasmic reticulum.

4 GO annotations of molecular function

Name Definition
1-phosphatidylinositol 4-kinase activity Catalysis of the reaction: 1-phosphatidyl-1D-myo-inositol + ATP = 1-phosphatidyl-1D-myo-inositol 4-phosphate + ADP + 2 H(+).
14-3-3 protein binding Binding to a 14-3-3 protein. A 14-3-3 protein is any of a large family of approximately 30kDa acidic proteins which exist primarily as homo- and heterodimers within all eukaryotic cells, and have been implicated in the modulation of distinct biological processes by binding to specific phosphorylated sites on diverse target proteins, thereby forcing conformational changes or influencing interactions between their targets and other molecules. Each 14-3-3 protein sequence can be roughly divided into three sections: a divergent amino terminus, the conserved core region and a divergent carboxy-terminus. The conserved middle core region of the 14-3-3s encodes an amphipathic groove that forms the main functional domain, a cradle for interacting with client proteins.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
phosphatidylinositol kinase activity Catalysis of the reaction: ATP + a phosphatidylinositol = ADP + a phosphatidylinositol phosphate.

5 GO annotations of biological process

Name Definition
lysosome organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a lysosome. A lysosome is a cytoplasmic, membrane-bounded organelle that is found in most animal cells and that contains a variety of hydrolases.
phosphatidylinositol phosphate biosynthetic process The chemical reactions and pathways resulting in the formation of phosphatidylinositol phosphate.
phosphatidylinositol-mediated signaling The series of molecular signals in which a cell uses a phosphatidylinositol-mediated signaling to convert a signal into a response. Phosphatidylinositols include phosphatidylinositol (PtdIns) and its phosphorylated derivatives.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
signal transduction The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.

8 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P39104 PIK1 Phosphatidylinositol 4-kinase PIK1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
O02810 PI4KB Phosphatidylinositol 4-kinase beta Bos taurus (Bovine) PR
Q9UBF8 PI4KB Phosphatidylinositol 4-kinase beta Homo sapiens (Human) PR
Q8BKC8 Pi4kb Phosphatidylinositol 4-kinase beta Mus musculus (Mouse) PR
O08662 Pi4ka Phosphatidylinositol 4-kinase alpha Rattus norvegicus (Rat) PR
Q9Z1L0 Pik3cb Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform Rattus norvegicus (Rat) PR
A4IID4 pi4kb Phosphatidylinositol 4-kinase beta Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) PR
Q49GP3 pi4kb Phosphatidylinositol 4-kinase beta Danio rerio (Zebrafish) (Brachydanio rerio) PR
10 20 30 40 50 60
MGDMVVEPAT LKPTSEPTPS PSGNNGGSLL SVITEGVGEL SVIDPEVAQK ACQEVLEKVK
70 80 90 100 110 120
LLHGGVAISS KGSPLELVNG DGVDNEIRCL DDPPTEIREE EDEMEPGVVS GTAKGTRRRR
130 140 150 160 170 180
QNNSAKQSWL LRLFESKLFD ISMAISYLYN SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL
190 200 210 220 230 240
NMYIHMDEDV GDAIKPYIVH RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI
250 260 270 280 290 300
LSDELKPAHR KRELPTLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN
310 320 330 340 350 360
EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ RLISELSLLN
370 380 390 400 410 420
HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY VEVLECENFD TTNVPARIPE
430 440 450 460 470 480
NRIRSTRSVE NLPECGITHE QRAGSFSTVP NYDNDDEAWS VDDIGELQVE LPEVHTNSCD
490 500 510 520 530 540
NISQFSVDSI TSQESKEPVF IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK
550 560 570 580 590 600
VRRIREGSPY GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI
610 620 630 640 650 660
LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ RNFVQSCAGY
670 680 690 700 710 720
CLVCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN LGFETSAFKL TTEFVDVMGG
730 740 750 760 770 780
LNGDMFNYYK MLMLQGLIAA RKHMDKVVQI VEIMQQGSQL PCFHGSSTIR NLKERFHMSM
790 800 810
TEEQLQLLVE QMVDGSMRSI TTKLYDGFQY LTNGIM