O05581
Gene name |
Rv0998 |
Protein name |
Acetyltransferase Pat |
Names |
GCN5-like enzyme, GCN5-related N-acetyltransferase, GNAT, Protein acetyltransferase, Pat |
Species |
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
KEGG Pathway |
mtu:Rv0998 |
EC number |
|
Protein Class |
|
Descriptions
Acetyltransferase Pat is a single Gcn5-like enzyme, in which Gcn5-like PAT domain enables direct cAMP control of protein acetylation. The crystal structures of the autoinhibited and cAMP-activated Pat reveal that cAMP binds to a cryptic site in the regulatory domain. An extensive conformational rearrangement relieves the autoinhibition by means of a substrate-mimicking lid that covers the protein-substrate binding surface.
Autoinhibitory domains (AIDs)
Target domain |
156-289 (Acetyltransferase (GNAT) domain) |
Relief mechanism |
Ligand binding |
Assay |
Structural analysis |
Target domain |
156-289 (Acetyltransferase (GNAT) domain) |
Relief mechanism |
Ligand binding |
Assay |
Structural analysis |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
4 structures for O05581
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 4AVA | X-ray | 170 A | A | 1-333 | PDB |
| 4AVB | X-ray | 180 A | A/B | 1-333 | PDB |
| 4AVC | X-ray | 281 A | A/B | 1-333 | PDB |
| AF-O05581-F1 | Predicted | AlphaFoldDB |
No variants for O05581
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for O05581 | |||||
No associated diseases with O05581
14 regional properties for O05581
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | PB1 domain | 25 - 107 | IPR000270 |
| domain | Protein kinase domain | 253 - 521 | IPR000719 |
| domain | AGC-kinase, C-terminal | 522 - 593 | IPR000961 |
| domain | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain | 140 - 192 | IPR002219 |
| active_site | Serine/threonine-protein kinase, active site | 373 - 385 | IPR008271 |
| binding_site | Protein kinase, ATP binding site | 259 - 286 | IPR017441 |
| domain | Protein kinase, C-terminal | 545 - 583 | IPR017892 |
| domain | Diacylglycerol/phorbol-ester binding | 138 - 152 | IPR020454-1 |
| domain | Diacylglycerol/phorbol-ester binding | 154 - 163 | IPR020454-2 |
| domain | Diacylglycerol/phorbol-ester binding | 167 - 178 | IPR020454-3 |
| domain | Diacylglycerol/phorbol-ester binding | 179 - 191 | IPR020454-4 |
| domain | Atypical protein kinase C iota type, catalytic domain | 232 - 595 | IPR034661 |
| domain | Protein kinase C, PB1 domain | 26 - 108 | IPR034877 |
| domain | PB1-like domain | 25 - 108 | IPR053793 |
1 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| acetyltransferase activity | Catalysis of the transfer of an acetyl group to an acceptor molecule. |
| acyltransferase activity | Catalysis of the transfer of an acyl group from one compound (donor) to another (acceptor). |
| cAMP binding | Binding to cAMP, the nucleotide cyclic AMP (adenosine 3',5'-cyclophosphate). |
| DNA-binding transcription factor activity | A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons. |
| metal ion binding | Binding to a metal ion. |
No GO annotations of biological process
| Name | Definition |
|---|---|
| No GO annotations for biological process |
No homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| No homologous proteins | ||||
| 10 | 20 | 30 | 40 | 50 | 60 |
| MDGIAELTGA | RVEDLAGMDV | FQGCPAEGLV | SLAASVQPLR | AAAGQVLLRQ | GEPAVSFLLI |
| 70 | 80 | 90 | 100 | 110 | 120 |
| SSGSAEVSHV | GDDGVAIIAR | ALPGMIVGEI | ALLRDSPRSA | TVTTIEPLTG | WTGGRGAFAT |
| 130 | 140 | 150 | 160 | 170 | 180 |
| MVHIPGVGER | LLRTARQRLA | AFVSPIPVRL | ADGTQLMLRP | VLPGDRERTV | HGHIQFSGET |
| 190 | 200 | 210 | 220 | 230 | 240 |
| LYRRFMSARV | PSPALMHYLS | EVDYVDHFVW | VVTDGSDPVA | DARFVRDETD | PTVAEIAFTV |
| 250 | 260 | 270 | 280 | 290 | 300 |
| ADAYQGRGIG | SFLIGALSVA | ARVDGVERFA | ARMLSDNVPM | RTIMDRYGAV | WQREDVGVIT |
| 310 | 320 | 330 | |||
| TMIDVPGPGE | LSLGREMVDQ | INRVARQVIE | AVG |