AiPD: Autoinhibited Protein Database

O00585

Gene name	CCL21 (SCYA21, UNQ784/PRO1600)
Protein name	C-C motif chemokine 21
Names	6Ckine, Beta-chemokine exodus-2, Secondary lymphoid-tissue chemokine, SLC, Small-inducible cytokine A21
Species	Homo sapiens (human)
KEGG Pathway	hsa:6366
EC number
Protein Class	SMALL INDUCIBLE CYTOKINE A (PTHR12015)

Descriptions

C-C motif chemokine 21 (CCL21) is chemokine that inhibits hemopoiesis and stimulates chemotaxis, and may play a role in mediating homing of lymphocytes to secondary lymphoid organ. CCL21 has an unstructured C-terminus that does not adopt a stable fold. This C-terminal tail of CCL21 makes autoinhibitory contacts with and modulates the structure and function of the CCL21 chemokine domain. CCL21's C-terminus transiently interacts with the chemokine domain of CCL21, thereby providing a putative structural correlate of functional autoinhibition.

Autoinhibitory domains (AIDs)

Target domain	1-79 (Chemokine domain)
Relief mechanism	PTM
Assay	Deletion assay, Structural analysis

AID

80-134 (C-terminal tail)

Target domain

1-79 (Chemokine domain)

Relief mechanism

PTM (Polysialylation of C-terminal tail)

Assay

Deletion assay, Structural analysis

Accessory elements

No accessory elements

References

Kiermaier E et al. (2016) "Polysialylation controls dendritic cell trafficking by regulating chemokine recognition", Science (New York, N.Y.), 351, 186-90

Smith EW et al. (2016) "Crystallographic Structure of Truncated CCL21 and the Putative Sulfotyrosine-Binding Site", Biochemistry, 55, 5746-5753

Autoinhibited structure

Activated structure

3 structures for O00585

Entry ID	Method	Resolution	Chain	Position	Source
2L4N	NMR	-	A	24-134	PDB
5EKI	X-ray	190 A	A/B/C/D/E/F	24-102	PDB
AF-O00585-F1	Predicted				AlphaFoldDB

117 variants for O00585

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
CA373273735 COSM1319603 rs1435524206	2	A>V	haematopoietic_and_lymphoid_tissue [Cosmic]	No	ClinGen cosmic curated TOPMed
rs376939263 CA5037155	4	S>*		No	ClinGen ESP ExAC gnomAD
rs1413276539 CA373273656	6	A>V		No	ClinGen gnomAD
CA192694192 rs779706400	7	L>P		No	ClinGen Ensembl
rs766751281 CA5037154	8	S>G		No	ClinGen ExAC gnomAD
CA192694190 rs901577787	8	S>R		No	ClinGen TOPMed gnomAD
CA373273479 rs1201441537	15	A>T		No	ClinGen gnomAD
rs1485651001 CA373273461	16	F>L		No	ClinGen gnomAD
CA192694183 rs187539558	16	F>S		No	ClinGen 1000Genomes
rs372498578 CA192694179	17	G>S		No	ClinGen ESP TOPMed
rs750926648 CA5037152	19	P>H		No	ClinGen ExAC
rs1259222173 CA373273358	20	R>S		No	ClinGen gnomAD
CA373273350 rs1212746799	21	T>A		No	ClinGen gnomAD
rs768165822 CA373273338	21	T>I		No	ClinGen ExAC gnomAD
rs768165822 CA5037151	21	T>N		No	ClinGen ExAC gnomAD
CA373273314 rs1329960763	22	Q>H		No	ClinGen gnomAD
rs1332010028 CA373273292	23	G>S		No	ClinGen TOPMed gnomAD
CA373272501 rs1433326878	24	S>R		No	ClinGen gnomAD
CA373272480 rs1435356127	25	D>Y		No	ClinGen gnomAD
CA373272391 rs1323280490	29	Q>*		No	ClinGen gnomAD
rs768083949 CA5037134	29	Q>H		No	ClinGen ExAC gnomAD
CA5037135 rs750836820	29	Q>L		No	ClinGen ExAC gnomAD
rs753133670 CA192693980	30	D>Y		No	ClinGen Ensembl
CA373272202 rs1164366610	36	S>G		No	ClinGen TOPMed gnomAD
rs762419173 CA5037133	38	R>K		No	ClinGen ExAC gnomAD
rs1371709137 CA373272121 COSM1314790	39	K>N	urinary_tract [Cosmic]	No	ClinGen cosmic curated gnomAD
TCGA novel	39	K>R	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
TCGA novel	40	I>F	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs142482921 CA5037130	42	A>S		No	ClinGen ESP ExAC TOPMed gnomAD
rs142482921 CA373272080	42	A>T		No	ClinGen ESP ExAC TOPMed gnomAD
CA373272044 rs969995486	43	K>N		No	ClinGen TOPMed gnomAD
rs1435390901 CA373272066	43	K>Q		No	ClinGen TOPMed
rs865854292 CA192693934	44	V>I		No	ClinGen Ensembl
rs1453433779 CA373271981	46	R>C	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA gnomAD
rs1453433779 CA373271977	46	R>G		No	ClinGen gnomAD
CA5037129 rs776318372	46	R>H		No	ClinGen ExAC TOPMed gnomAD
rs1195160505 CA373271946	47	S>G		No	ClinGen TOPMed gnomAD
CA5037127 rs760414514	49	R>Q		No	ClinGen ExAC TOPMed gnomAD
CA5037128 rs765979271	49	R>W		No	ClinGen ExAC TOPMed gnomAD
rs539376444 CA5037124	53	P>R		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs771921191 CA5037125	53	P>S		No	ClinGen ExAC gnomAD
CA373271768 rs771921191	53	P>T		No	ClinGen ExAC gnomAD
CA5037123 rs774203687	56	G>D		No	ClinGen ExAC gnomAD
rs780269897 CA5037120	59	I>N		No	ClinGen ExAC
CA5037121 rs749355595 COSM1108516	59	I>V	Variant assessed as Somatic; 0.0 impact. endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated ExAC NCI-TCGA gnomAD
rs1371156951 CA373271610	60	P>L		No	ClinGen TOPMed
CA192693815 rs201778181	60	P>S		No	ClinGen Ensembl
CA373271554 rs1170851787	62	I>N		No	ClinGen gnomAD
CA373271275 rs1283173183	66	P>L		No	ClinGen gnomAD
rs753313552 CA5037095	66	P>S		No	ClinGen ExAC TOPMed gnomAD
rs753313552 CA5037096	66	P>T		No	ClinGen ExAC TOPMed gnomAD
CA5037092 rs750044894	67	R>C		No	ClinGen ExAC TOPMed gnomAD
rs34598986 CA5037090	67	R>H		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs34598986 CA5037091	67	R>L		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs764022594 CA5037088	68	K>N		No	ClinGen ExAC gnomAD
CA373271198 rs1437686948	68	K>R		No	ClinGen TOPMed gnomAD
CA5037087 rs762798553	69	R>C		No	ClinGen ExAC TOPMed gnomAD
CA5037086 rs775560130 COSM1247596	69	R>H	oesophagus [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
rs762798553 CA373271180	69	R>S		No	ClinGen ExAC TOPMed gnomAD
CA5037085 rs769975656	72	A>E		No	ClinGen ExAC gnomAD
rs1359922266 CA373271097	72	A>T		No	ClinGen TOPMed gnomAD
rs1390934171 CA373271064	74	L>I		No	ClinGen gnomAD
rs759733358 CA5037084	75	C>R		No	ClinGen ExAC gnomAD
CA5037083 rs776954599	75	C>S		No	ClinGen ExAC TOPMed gnomAD
CA373271013 rs1268054875	76	A>T		No	ClinGen TOPMed
CA192693454 rs956153540	76	A>V		No	ClinGen TOPMed gnomAD
CA373270965 rs1473024111	78	P>S		No	ClinGen gnomAD
rs778006557 CA373270884	80	E>D		No	ClinGen ExAC TOPMed gnomAD
rs778006557 CA5037080	80	E>D		No	ClinGen ExAC TOPMed gnomAD
rs1451078274 CA373270854	82	W>*		No	ClinGen TOPMed
CA373270829 rs1182863895	83	V>A		No	ClinGen TOPMed
TCGA novel	87	M>K	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
COSM379761 CA5037079 rs772545076	88	Q>*	lung [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed
CA192693400 rs772545076	88	Q>K		No	ClinGen ExAC TOPMed
CA192693374 rs939141004	89	H>Q		No	ClinGen TOPMed
CA5037077 rs748581651	89	H>R		No	ClinGen ExAC TOPMed gnomAD
CA373270673 rs1334728950	90	L>V		No	ClinGen gnomAD
CA5037074 rs755580810	92	K>R		No	ClinGen ExAC gnomAD
CA5037073 rs749954967	93	T>A		No	ClinGen ExAC TOPMed gnomAD
rs1391448419 CA373270544	95	S>F		No	ClinGen TOPMed
rs1161356955 CA373270562	95	S>T		No	ClinGen TOPMed
rs1391448419 CA373270558	95	S>Y		No	ClinGen TOPMed
CA373270504 rs1408399469	97	Q>R		No	ClinGen TOPMed
CA192693334 rs915935603	99	P>L		No	ClinGen gnomAD
rs1336150097 CA373270386	101	Q>H		No	ClinGen TOPMed
rs751296236 CA5037070	103	C>R		No	ClinGen ExAC gnomAD
rs762857659 CA5037068	104	R>S		No	ClinGen ExAC TOPMed gnomAD
CA373270274 rs1166542420	105	K>*		No	ClinGen gnomAD
CA373270233 rs1450916503	106	D>E		No	ClinGen gnomAD
rs752548751 CA5037067	107	R>M		No	ClinGen ExAC TOPMed gnomAD
rs752548751 CA373270212	107	R>T		No	ClinGen ExAC TOPMed gnomAD
CA373270198 rs1564116483	108	G>E		No	ClinGen Ensembl
rs777471870	109	A>P	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA5037066 rs759642265	109	A>S		No	ClinGen ExAC TOPMed gnomAD
CA5037065 rs759642265	109	A>T		No	ClinGen ExAC TOPMed gnomAD
rs776864576 CA5037063	110	S>F		No	ClinGen ExAC TOPMed gnomAD
CA373270096 rs1322924992	111	K>N		No	ClinGen TOPMed gnomAD
CA5037062 rs771211630	111	K>R		No	ClinGen ExAC gnomAD
rs760793347 CA5037061	113	G>R		No	ClinGen ExAC gnomAD
CA373270007 rs1257922234	114	K>R		No	ClinGen TOPMed
CA373269805 rs772291642	121	G>A		No	ClinGen ExAC TOPMed gnomAD
COSM1624932 CA5037060 rs773531936	121	G>S	liver [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
rs772291642 CA5037059	121	G>V		No	ClinGen ExAC TOPMed gnomAD
CA373269712 rs368229543	124	R>G		No	ClinGen ESP TOPMed gnomAD
rs779340021 CA5037056	124	R>K		No	ClinGen ExAC TOPMed gnomAD
CA373269596 rs1219925355	124	R>S		No	ClinGen gnomAD
rs754971604 CA5037044	126	E>K		No	ClinGen ExAC TOPMed gnomAD
rs148496138 CA5037042 COSM1734322	127	R>Q	pancreas [Cosmic]	No	ClinGen cosmic curated 1000Genomes ESP ExAC TOPMed gnomAD
rs1370944166 CA373269500	128	S>*	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA gnomAD
rs576393548 CA5037041	129	Q>E		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1191474871 CA373269479	129	Q>R		No	ClinGen gnomAD
CA5037040 rs374347595	130	T>I		No	ClinGen ESP ExAC gnomAD
CA373269431 rs1360588383	131	P>S		No	ClinGen gnomAD
rs1301405103 CA373269415	132	K>E		No	ClinGen TOPMed
rs767802042 CA5037039	132	K>N		No	ClinGen ExAC gnomAD
CA192693140 rs1007358262	133	G>R		No	ClinGen Ensembl
rs762022123 CA5037038	134	P>S		No	ClinGen ExAC gnomAD

No associated diseases with O00585

8 regional properties for O00585

Type	Name	Position	InterPro Accession
domain	SH3 domain	8 - 69	IPR001452
domain	Dedicator of cytokinesis protein 2, DHR2 domain	1200 - 1620	IPR026799
domain	C2 DOCK-type domain	419 - 615	IPR027007
domain	DOCKER domain	1211 - 1622	IPR027357
domain	Dedicator of cytokinesis, N-terminal domain	72 - 414	IPR032376
domain	DOCKER, Lobe A	1205 - 1334	IPR046769
domain	DOCKER, Lobe B	1395 - 1474	IPR046770
domain	DOCKER, Lobe C	1516 - 1615	IPR046773

Functions

		Description
EC Number
Subcellular Localization	Secreted
PANTHER Family	PTHR12015	SMALL INDUCIBLE CYTOKINE A
PANTHER Subfamily	PTHR12015:SF72	C-C MOTIF CHEMOKINE 21
PANTHER Protein Class	cytokine
PANTHER Pathway Category	Inflammation mediated by chemokine and cytokine signaling pathway CHK

2 GO annotations of cellular component

Name	Definition
extracellular region	The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
extracellular space	That part of a multicellular organism outside the cells proper, usually taken to be outside the plasma membranes, and occupied by fluid.

4 GO annotations of molecular function

Name	Definition
CCR chemokine receptor binding	Binding to a CCR chemokine receptor.
CCR7 chemokine receptor binding	Binding to a CCR7 chemokine receptor.
chemokine activity	The function of a family of small chemotactic cytokines; their name is derived from their ability to induce directed chemotaxis in nearby responsive cells. All chemokines possess a number of conserved cysteine residues involved in intramolecular disulfide bond formation. Some chemokines are considered pro-inflammatory and can be induced during an immune response to recruit cells of the immune system to a site of infection, while others are considered homeostatic and are involved in controlling the migration of cells during normal processes of tissue maintenance or development. Chemokines are found in all vertebrates, some viruses and some bacteria.
chemokine receptor binding	Binding to a chemokine receptor.

51 GO annotations of biological process

Name	Definition
activation of GTPase activity	Any process that initiates the activity of an inactive GTPase through the replacement of GDP by GTP.
antimicrobial humoral immune response mediated by antimicrobial peptide	An immune response against microbes mediated by anti-microbial peptides in body fluid.
cell chemotaxis	The directed movement of a motile cell guided by a specific chemical concentration gradient. Movement may be towards a higher concentration (positive chemotaxis) or towards a lower concentration (negative chemotaxis).
cell maturation	A developmental process, independent of morphogenetic (shape) change, that is required for a cell to attain its fully functional state.
cell-cell signaling	Any process that mediates the transfer of information from one cell to another. This process includes signal transduction in the receiving cell and, where applicable, release of a ligand and any processes that actively facilitate its transport and presentation to the receiving cell. Examples include signaling via soluble ligands, via cell adhesion molecules and via gap junctions.
cellular response to chemokine	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a chemokine stimulus.
cellular response to interleukin-1	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an interleukin-1 stimulus.
cellular response to tumor necrosis factor	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a tumor necrosis factor stimulus.
cellular response to type II interferon	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an interferon-gamma stimulus. Interferon gamma is the only member of the type II interferon found so far.
chemokine (C-C motif) ligand 21 signaling pathway	The series of molecular signals initiated by the binding of the C-C chemokine CCL21 to its receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription.
chemokine-mediated signaling pathway	The series of molecular signals initiated by a chemokine binding to its receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription.
dendritic cell chemotaxis	The movement of a dendritic cell in response to an external stimulus.
dendritic cell dendrite assembly	Formation of dendrites, branched cellular projections (or cytoplasmic extension) that are extended from the surface of a dendritic immune cell, and which enable the cell to sample luminal pathogens and increase the surface area for antigen presentation to T cells.
eosinophil chemotaxis	The movement of an eosinophil in response to an external stimulus.
establishment of T cell polarity	The directed orientation of T cell signaling molecules and associated membrane rafts towards a chemokine gradient or a contact point with antigen presenting cell.
G protein-coupled receptor signaling pathway	The series of molecular signals initiated by a ligand binding to its receptor, in which the activated receptor promotes the exchange of GDP for GTP on the alpha-subunit of an associated heterotrimeric G-protein complex. The GTP-bound activated alpha-G-protein then dissociates from the beta- and gamma-subunits to further transmit the signal within the cell. The pathway begins with receptor-ligand interaction, and ends with regulation of a downstream cellular process. The pathway can start from the plasma membrane, Golgi or nuclear membrane.
immune response	Any immune system process that functions in the calibrated response of an organism to a potential internal or invasive threat.
immunological synapse formation	The formation of an area of close contact between a lymphocyte (T-, B-, or natural killer cell) and a target cell through the clustering of particular signaling and adhesion molecules and their associated membrane rafts on both the lymphocyte and target cell, which facilitates activation of the lymphocyte, transfer of membrane from the target cell to the lymphocyte, and in some situations killing of the target cell through release of secretory granules and/or death-pathway ligand-receptor interaction.
inflammatory response	The immediate defensive reaction (by vertebrate tissue) to infection or injury caused by chemical or physical agents. The process is characterized by local vasodilation, extravasation of plasma into intercellular spaces and accumulation of white blood cells and macrophages.
killing of cells of another organism	Any process in an organism that results in the killing of cells of another organism, including in some cases the death of the other organism. Killing here refers to the induction of death in one cell by another cell, not cell-autonomous death due to internal or other environmental conditions.
lymphocyte chemotaxis	The directed movement of a lymphocyte in response to an external stimulus.
mesangial cell-matrix adhesion	The binding of a mesangial cell to the extracellular matrix via adhesion molecules. A mesangial cell is a cell that encapsulates the capillaries and venules in the kidney.
monocyte chemotaxis	The movement of a monocyte in response to an external stimulus.
negative regulation of dendritic cell apoptotic process	Any process that stops, prevents or reduces the frequency, rate or extent of dendritic cell apoptotic process.
negative regulation of dendritic cell dendrite assembly	Any process that stops, prevents or reduces the frequency, rate or extent of dendritic cell dendrite assembly.
negative regulation of leukocyte tethering or rolling	Any process that stops, prevents or reduces the frequency, rate or extent of leukocyte tethering or rolling.
neutrophil chemotaxis	The directed movement of a neutrophil cell, the most numerous polymorphonuclear leukocyte found in the blood, in response to an external stimulus, usually an infection or wounding.
positive regulation of actin filament polymerization	Any process that activates or increases the frequency, rate or extent of actin polymerization.
positive regulation of cell adhesion mediated by integrin	Any process that activates or increases the frequency, rate, or extent of cell adhesion mediated by integrin.
positive regulation of cell motility	Any process that activates or increases the frequency, rate or extent of cell motility.
positive regulation of cell-matrix adhesion	Any process that activates or increases the rate or extent of cell adhesion to an extracellular matrix.
positive regulation of chemotaxis	Any process that activates or increases the frequency, rate or extent of the directed movement of a motile cell or organism in response to a specific chemical concentration gradient.
positive regulation of dendritic cell antigen processing and presentation	Any process that activates or increases the frequency, rate, or extent of dendritic cell antigen processing and presentation.
positive regulation of ERK1 and ERK2 cascade	Any process that activates or increases the frequency, rate or extent of signal transduction mediated by the ERK1 and ERK2 cascade.
positive regulation of filopodium assembly	Any process that activates or increases the frequency, rate or extent of the assembly of a filopodium, a thin, stiff protrusion extended by the leading edge of a motile cell such as a crawling fibroblast or amoeba, or an axonal growth cone.
positive regulation of glycoprotein biosynthetic process	Any process that increases the rate, frequency, or extent of the chemical reactions and pathways resulting in the formation of a glycoprotein, a protein that contains covalently bound glycose (i.e. monosaccharide) residues; the glycose occurs most commonly as oligosaccharide or fairly small polysaccharide but occasionally as monosaccharide.
positive regulation of GTPase activity	Any process that activates or increases the activity of a GTPase.
positive regulation of I-kappaB kinase/NF-kappaB signaling	Any process that activates or increases the frequency, rate or extent of I-kappaB kinase/NF-kappaB signaling.
positive regulation of JNK cascade	Any process that activates or increases the frequency, rate or extent of signal transduction mediated by the JNK cascade.
positive regulation of myeloid dendritic cell chemotaxis	Any process that activates or increases the frequency, rate or extent of myeloid dendritic cell chemotaxis.
positive regulation of neutrophil chemotaxis	Any process that increases the frequency, rate, or extent of neutrophil chemotaxis. Neutrophil chemotaxis is the directed movement of a neutrophil cell, the most numerous polymorphonuclear leukocyte found in the blood, in response to an external stimulus, usually an infection or wounding.
positive regulation of phosphatidylinositol 3-kinase activity	Any process that activates or increases the frequency, rate or extent of phosphatidylinositol 3-kinase activity.
positive regulation of protein kinase activity	Any process that activates or increases the frequency, rate or extent of protein kinase activity.
positive regulation of protein kinase B signaling	Any process that activates or increases the frequency, rate or extent of protein kinase B signaling, a series of reactions mediated by the intracellular serine/threonine kinase protein kinase B.
positive regulation of pseudopodium assembly	Any process that activates or increases the frequency, rate or extent of the assembly of pseudopodia.
positive regulation of receptor-mediated endocytosis	Any process that activates or increases the frequency, rate or extent of receptor mediated endocytosis, the uptake of external materials by cells, utilizing receptors to ensure specificity of transport.
positive regulation of T cell chemotaxis	Any process that increases the rate, frequency or extent of T cell chemotaxis. T cell chemotaxis is the directed movement of a T cell in response to an external stimulus.
positive regulation of T cell migration	Any process that activates or increases the frequency, rate or extent of T cell migration.
release of sequestered calcium ion into cytosol	The process in which calcium ions sequestered in the endoplasmic reticulum, Golgi apparatus or mitochondria are released into the cytosolic compartment.
response to prostaglandin E	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a prostagladin E stimulus.
ruffle organization	A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a ruffle, a projection at the leading edge of a crawling cell.

4 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
P84444	Ccl21a	C-C motif chemokine 21a	Mus musculus (Mouse)	SS
P86792	Ccl21b	C-C motif chemokine 21b	Mus musculus (Mouse)	SS
P86793	Ccl21c	C-C motif chemokine 21c	Mus musculus (Mouse)	SS
Q8HYP5	CCL21	C-C motif chemokine 21	Macaca mulatta (Rhesus macaque)	SS

10	20	30	40	50	60
MAQSLALSLL	ILVLAFGIPR	TQGSDGGAQD	CCLKYSQRKI	PAKVVRSYRK	QEPSLGCSIP
70	80	90	100	110	120
AILFLPRKRS	QAELCADPKE	LWVQQLMQHL	DKTPSPQKPA	QGCRKDRGAS	KTGKKGKGSK
130
GCKRTERSQT	PKGP