M9PE65

SS
Gene name
axo (CG13715, CG13717, CG18296, CG32234, CG42664, CT1277, CG43225)
Protein name
Axotactin
Names
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG43225
EC number
Protein Class

Descriptions

Kunitz-type protease inhibitor 2 (or hepatocyte growth factor activator inhibitor type 2 (HAI-2)) is an integral membrane Kunitz-type serine protease inhibitor that regulates the proteolysis of matriptase and prostasin in a cell-type selective manner. The vast majority of non-glycosylated HAI-2 is synthesized into multiple disulfide-linked oligomers, which lack protease inhibitory function, likely due to distorted conformations caused by the disarrayed disulfide linkages. N-glycosylation on Asn-57 is required for folding into a functional HAI-2 with full protease suppressive activity and correct subcellular targeting signal.

Autoinhibitory domains (AIDs)

138-139 (N-glycosylation site) SS

Target domain

116-171 (Pancreatic trypsin inhibitor Kunitz domain)

Relief mechanism

PTM

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for M9PE65

Entry ID Method Resolution Chain Position Source
AF-M9PE65-F1 Predicted AlphaFoldDB

No variants for M9PE65

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for M9PE65

No associated diseases with M9PE65

11 regional properties for M9PE65

Type Name Position InterPro Accession
domain EGF-like domain 439 - 477 IPR000742-1
domain EGF-like domain 841 - 879 IPR000742-2
domain EGF-like domain 1260 - 1297 IPR000742-3
domain EGF-like domain 1527 - 1565 IPR000742-4
domain Laminin G domain 242 - 438 IPR001791-1
domain Laminin G domain 481 - 664 IPR001791-2
domain Laminin G domain 660 - 839 IPR001791-3
domain Laminin G domain 1087 - 1259 IPR001791-4
domain Laminin G domain 1316 - 1526 IPR001791-5
domain Laminin G domain 1569 - 1765 IPR001791-6
domain Pancreatic trypsin inhibitor Kunitz domain 117 - 171 IPR002223

Functions

Description
EC Number
Subcellular Localization
  • Cell projection, axon
  • Membrane ; Single-pass type I membrane protein
  • Localizes to longitudinal axon tracts as well as to an axonal scaffold in the brain (PubMed:10037607)
  • A secreted form might exist (Probable)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
axon The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.

1 GO annotations of molecular function

Name Definition
serine-type endopeptidase inhibitor activity Binds to and stops, prevents or reduces the activity of a serine-type endopeptidase.

1 GO annotations of biological process

Name Definition
transmission of nerve impulse The neurological system process in which a signal is transmitted through the nervous system by a combination of action potential propagation and synaptic transmission.

15 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q7YRQ8 TFPI2 Tissue factor pathway inhibitor 2 Bos taurus (Bovine) SS
Q868Z9 Ppn Papilin Drosophila melanogaster (Fruit fly) SS
P49223 SPINT3 Kunitz-type protease inhibitor 3 Homo sapiens (Human) SS
P48307 TFPI2 Tissue factor pathway inhibitor 2 Homo sapiens (Human) SS
P10646 TFPI Tissue factor pathway inhibitor Homo sapiens (Human) SS
O43291 SPINT2 Kunitz-type protease inhibitor 2 Homo sapiens (Human) EV
O43278 SPINT1 Kunitz-type protease inhibitor 1 Homo sapiens (Human) EV
P78357 CNTNAP1 Contactin-associated protein 1 Homo sapiens (Human) PR
Q9R097 Spint1 Kunitz-type protease inhibitor 1 Mus musculus (Mouse) SS
O35536 Tfpi2 Tissue factor pathway inhibitor 2 Mus musculus (Mouse) SS
Q9WU03 Spint2 Kunitz-type protease inhibitor 2 Mus musculus (Mouse) SS
O54819 Tfpi Tissue factor pathway inhibitor Mus musculus (Mouse) SS
Q0V8T9 Cntnap5a Contactin-associated protein like 5-1 Mus musculus (Mouse) PR
Q02445 Tfpi Tissue factor pathway inhibitor Rattus norvegicus (Rat) SS
Q28864 TFPI Tissue factor pathway inhibitor Macaca mulatta (Rhesus macaque) SS
10 20 30 40 50 60
MAFPYIWALL PLICSASGLS LPNMTSTDAV VAGGGILPIL VAGNPGNLGS SNMSLSGGGG
70 80 90 100 110 120
LAGSSTGGQS LPDTGGGNSA GGSPAGGSSG TGGGGSNSGI SGNNSAMIQG QKSNQYEKCA
130 140 150 160 170 180
GPGDPGPCKQ YIYKWRYEPT TNECTNFIWG GCEGNPQNRF GTEAECLFHC IGGPHTLPPF
190 200 210 220 230 240
LQSTTREPST TESSMLLGLP YTQSPAQSPD GMGGAEGGDG TTPVPIEQRG PELTFAETGQ
250 260 270 280 290 300
GKTFIFAKNN TFIQMDGDII QTFQLRLCRE ISFQFRTRLP HGLLVYHNVK NPDRINLDPY
310 320 330 340 350 360
ALYVIVEKGQ LKVVHVFGKH STSVTVGESL NRDEWHSVMV RIDVHGARLI ARVDNSQEEV
370 380 390 400 410 420
YLKGLNHEYN YGVSTNLPSV VLVGGLSSEE KLHGVKYITE SFVGCIRNVV LSSGKAASDL
430 440 450 460 470 480
LPIAPLVATK HENVNEGCSD MCESRHNLCF VGSRCINHYG GISCDCFGTH YEGEHCDIYT
490 500 510 520 530 540
ATIITLRGAS YVSYRIYDWK DRVHSSTRRI SLMFRTNFDD SALFYASGES LKHQYIAASI
550 560 570 580 590 600
KNQSVHVEMD FGDNVMSTVL TDDLTRGYWH NLTILHEQRT VSIILDQQQK VLELPATASG
610 620 630 640 650 660
NMLFDPEIYF GGGPELHKKK GLASHNNFVG SLKYVYYNDI SILYELQRGN PKVHYHGVLE
670 680 690 700 710 720
AEFVENEVNV IPITYPFATS HIWWPINHAE EFNIKFDFRS SRPGAVLAYS DVTTSAGNGF
730 740 750 760 770 780
WEIRLTSDKL SFDLVPDVNN NVTHSTTIKI NRATSWHSVE LDYKLGEIRF TVDYRHTLSQ
790 800 810 820 830 840
MYGLTFNIGD KLIIGSSLKS AAMGLVGCIR DIEINGHLIE PRHVVKTERV VGEVALDNCN
850 860 870 880 890 900
YIDPCKRPNT CEHGGKCFVK DDRVTCDCKH TGYIGKNCHF TKYRKTCEEL ALLGFTKSDV
910 920 930 940 950 960
YLIDIDGNGV FPPAHVKCDF QSLENATKTI VEHNLPSQVD VRSARESDFS FNIRYREFSP
970 980 990 1000 1010 1020
HMLQELISHS LYCTQYIKYD CYRAQLELHS ATWFTSSAKN LTVDFLGNVK RGACPCSVNK
1030 1040 1050 1060 1070 1080
TCVDPNQSCN CDVKENKWNS DEGYYQDPQS LGITNMYFLQ QKDMDDEAQG RITLGPLECV
1090 1100 1110 1120 1130 1140
ETNTQKYVVT FTTSQSYIEV PGWRKGDIAF SFRTTGEKAI LLFQPPIRPH YPSFMVALTG
1150 1160 1170 1180 1190 1200
DDQLTFTFTL STGTTRELVI NSHRRLNGGE WHKIWIDYNQ YHVRFMINTD YQMLDLLPEE
1210 1220 1230 1240 1250 1260
EFGPFEGSMY IGGATFDLLK KLSVKAGLIG CFRGLVVNGE ILDIYSYMSV HLSEIIKDCK
1270 1280 1290 1300 1310 1320
PSCVPSPCRN GAQCKELWSS FKCVCNNPWA HIGEFCETNI NEKALTFINR ESFLMRNYLS
1330 1340 1350 1360 1370 1380
VGATPVILMH GINGERDVLK GILNQDLLIN LRTYDTNALV LYANDHYNNF VHLYISLNRE
1390 1400 1410 1420 1430 1440
IVFLYNYGDE IVNLTLLDDT LMASLKSIQV AIVRGEQETR MHVNEHSVSI DRGTLLLDEY
1450 1460 1470 1480 1490 1500
ANKPWSNPEK EVLSPHRPPA PPTEYFQFHV GGYDPANLLR PNVDAPALEG YIGCVRGLKI
1510 1520 1530 1540 1550 1560
GAQLIDLADI NERNIAPTQE GVLPNCQIKC DAEPCKNGGT CQEHFAEQLS TCDCEHTSFL
1570 1580 1590 1600 1610 1620
GEFCSEEKGA DFSGESTLQR KFELPGTGRV DYVRLQLAFS SFDLRRANRI MLLMQTEAER
1630 1640 1650 1660 1670 1680
SYYLLLAITS DGYLQLEEDR DNGQTVGARI DRNFLNSARH SVYYVRNGTQ SQLFIDREQV
1690 1700 1710 1720 1730 1740
PLSEFAARVL TTGGDAGSNR VQIGGINSTD SRFAVFKSYS GCLSNIYIQV NGHVMKPLEE
1750 1760 1770 1780 1790 1800
YMLFTKSGAD NITVINPQGV RSAQCNAKFD VSEQPTQEPM VNVSMIPEPW VEEPPARVPY
1810 1820 1830 1840 1850 1860
IPRFVYDENK QEDSTQVVFL TLTSVFVIIV ICCLLEVYRS HLAYKKRIER ETDEDIIWSK
1870 1880 1890 1900 1910 1920
EQATKMHESP GVKAGLLGGV TAGSGNGLPP YTYKALPQED KKPGNGAPLV GILKNGSATP
1930 1940 1950 1960 1970 1980
SQPGTPTALS KNGDIASRIE EEEEEEDEAP AQKAAEKSGE NEEPPAKDTT ASEIKESQAQ
1990 2000 2010 2020 2030 2040
PPEQLAKDTT DASAAPKASK ETEAQAEPSE PSSQLNSAQN GQLAQMEQAA RGDEVQVPSL
2050 2060 2070 2080 2090 2100
PHPIQPPDAI FMPNLPQKAQ QRQPQEHKSR HKATDDTEAP KQQQQQQQQQ QSFDVATNAN
2110 2120 2130 2140 2150 2160
GSSLPASRVK NPEEPGGKSP LVPMRQHHSK VTRPPPPPTL FLENSLLQRQ FANPISYLGG
2170
PRLQPRSNRT SIDSILSLD