M3TYT0

SS
Gene name
ROCK2
Protein name
Rho-associated protein kinase 2
Names
EC 2.7.11.1 , Rho kinase 2 , Rho-associated, coiled-coil-containing protein kinase 2 , Rho-associated, coiled-coil-containing protein kinase II , ROCK-II , p164 ROCK-2
Species
Sus scrofa (Pig)
KEGG Pathway
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

Rho kinases (ROCKs) are serine/threonine kinases that are involved in many aspects of cell motility, from smooth-muscle contraction to cell migration and neurite outgrowth. ROCK2 contains an N-terminal kinase domain, Rho-binding domain (RBD), and PH domain. RBD and PH domains interact with the catalytic kinase domain for autoinhibition. Rho binding to RBD relieves the autoinhibition.

Autoinhibitory domains (AIDs)

971-1047 (RBD domain) SS

Target domain

107-392 (Catalytic kinase domain)

Relief mechanism

Partner binding

Assay

1150-1349 (PH domain) SS

Target domain

107-392 (Catalytic kinase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

231-255 (Activation loop from InterPro)

Target domain

39-417 (Protein Kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for M3TYT0

Entry ID Method Resolution Chain Position Source
AF-M3TYT0-F1 Predicted AlphaFoldDB

12 variants for M3TYT0

Variant ID(s) Position Change Description Diseaes Association Provenance
rs691859004 68 I>V No EVA
rs691166787 84 G>D No EVA
rs699583388 259 P>H No EVA
rs691354468 464 E>D No EVA
rs706581723 619 D>E No EVA
rs711374024 626 V>A No EVA
rs694764723 643 D>N No EVA
rs692952594 752 N>D No EVA
rs704479359 763 I>L No EVA
rs709331938 834 L>F No EVA
rs710361774 1056 M>V No EVA
rs696106696 1282 W>* No EVA

No associated diseases with M3TYT0

7 regional properties for M3TYT0

Type Name Position InterPro Accession
domain DNA topoisomerase, type IIA, domain A 11 - 507 IPR002205
repeat DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat 538 - 584 IPR006691-1
repeat DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat 588 - 637 IPR006691-2
repeat DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat 646 - 688 IPR006691-3
repeat DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat 692 - 737 IPR006691-4
repeat DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat 744 - 789 IPR006691-5
repeat DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat 793 - 839 IPR006691-6

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Cell membrane ; Peripheral membrane protein
  • Nucleus
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Cytoplasmic, and associated with actin microfilaments and the plasma membrane
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoskeleton A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
metal ion binding Binding to a metal ion.
protein serine kinase activity Catalysis of the reactions
Rho-dependent protein serine/threonine kinase activity Rho GTPase-dependent catalysis of the reaction
small GTPase binding Binding to a small monomeric GTPase.

16 GO annotations of biological process

Name Definition
actomyosin structure organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures containing both actin and myosin or paramyosin. The myosin may be organized into filaments.
aortic valve morphogenesis The process in which the structure of the aortic valve is generated and organized.
cellular response to transforming growth factor beta stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a transforming growth factor beta stimulus.
cortical actin cytoskeleton organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of actin-based cytoskeletal structures in the cell cortex, i.e. just beneath the plasma membrane.
embryonic morphogenesis The process in which anatomical structures are generated and organized during the embryonic phase. The embryonic phase begins with zygote formation. The end of the embryonic phase is organism-specific. For example, it would be at birth for mammals, larval hatching for insects and seed dormancy in plants.
mitotic cytokinesis A cell cycle process that results in the division of the cytoplasm of a cell after mitosis, resulting in the separation of the original cell into two daughter cells.
negative regulation of biomineral tissue development Any process that stops, prevents, or reduces the frequency, rate or extent of biomineral tissue development, the formation of hard tissues that consist mainly of inorganic compounds.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
positive regulation of centrosome duplication Any process that increases the frequency, rate or extent of centrosome duplication. Centrosome duplication is the replication of a centrosome, a structure comprised of a pair of centrioles and peri-centriolar material from which a microtubule spindle apparatus is organized.
positive regulation of protein localization to nucleus Any process that activates or increases the frequency, rate or extent of protein localization to nucleus.
regulation of actin cytoskeleton organization Any process that modulates the frequency, rate or extent of the formation, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins.
regulation of cell junction assembly Any process that modulates the frequency, rate or extent of cell junction assembly.
regulation of circadian rhythm Any process that modulates the frequency, rate or extent of a circadian rhythm. A circadian rhythm is a biological process in an organism that recurs with a regularity of approximately 24 hours.
Rho protein signal transduction The series of molecular signals within the cell that are mediated by a member of the Rho family of proteins switching to a GTP-bound active state.
rhythmic process Any process pertinent to the generation and maintenance of rhythms in the physiology of an organism.
smooth muscle contraction A process in which force is generated within smooth muscle tissue, resulting in a change in muscle geometry. Force generation involves a chemo-mechanical energy conversion step that is carried out by the actin/myosin complex activity, which generates force through ATP hydrolysis. Smooth muscle differs from striated muscle in the much higher actin/myosin ratio, the absence of conspicuous sarcomeres and the ability to contract to a much smaller fraction of its resting length.

15 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q28021 ROCK2 Rho-associated protein kinase 2 Bos taurus (Bovine) SS
Q9W1B0 gek Serine/threonine-protein kinase Genghis Khan Drosophila melanogaster (Fruit fly) SS
Q9Y5S2 CDC42BPB Serine/threonine-protein kinase MRCK beta Homo sapiens (Human) SS
Q5VT25 CDC42BPA Serine/threonine-protein kinase MRCK alpha Homo sapiens (Human) EV
Q13464 ROCK1 Rho-associated protein kinase 1 Homo sapiens (Human) SS
O75116 ROCK2 Rho-associated protein kinase 2 Homo sapiens (Human) SS
Q80UW5 Cdc42bpg Serine/threonine-protein kinase MRCK gamma Mus musculus (Mouse) PR
Q7TT50 Cdc42bpb Serine/threonine-protein kinase MRCK beta Mus musculus (Mouse) SS
P70335 Rock1 Rho-associated protein kinase 1 Mus musculus (Mouse) SS
Q3UU96 Cdc42bpa Serine/threonine-protein kinase MRCK alpha Mus musculus (Mouse) SS
P70336 Rock2 Rho-associated protein kinase 2 Mus musculus (Mouse) SS
O54874 Cdc42bpa Serine/threonine-protein kinase MRCK alpha Rattus norvegicus (Rat) SS
Q7TT49 Cdc42bpb Serine/threonine-protein kinase MRCK beta Rattus norvegicus (Rat) SS
Q63644 Rock1 Rho-associated protein kinase 1 Rattus norvegicus (Rat) SS
Q62868 Rock2 Rho-associated protein kinase 2 Rattus norvegicus (Rat) EV
10 20 30 40 50 60
MSRPPPTGKM PGAPEAVSGD GAGASRQRKL EALIRDPRSP INVESLLDGL NSLVLDLDFP
70 80 90 100 110 120
ALRKNKNIDN FLNRYEKIVK KIRGLQMKAE DYDVVKVIGR GAFGEVQLVR HKASQKVYAM
130 140 150 160 170 180
KLLSKFEMIK RSDSAFFWEE RDIMAFANSP WVVQLFCAFQ DDKYLYMVME YMPGGDLVNL
190 200 210 220 230 240
MSNYDVPEKW AKFYTAEVVL ALDAIHSMGL IHRDVKPDNM LLDKHGHLKL ADFGTCMKMD
250 260 270 280 290 300
ETGMVHCDTA VGTPDYISPE VLKSQGGDGY YGRECDWWSV GVFLFEMLVG DTPFYADSLV
310 320 330 340 350 360
GTYSKIMDHK NSLCFPEDAE ISKHAKNLIC AFLTDREVRL GRNGVEEIKQ HPFFKNDQWN
370 380 390 400 410 420
WDNIRETAAP VVPELSSDID SSNFDDIEDD KGDVETFPIP KAFVGNQLPF IGFTYYRENL
430 440 450 460 470 480
LLSDSPSCKE NDSIQIRKNE ESQEIQKKLY TLEEHLSTEI QAKEELEQKC KSVNTRLEKV
490 500 510 520 530 540
AKELEEEIAL RKNVESALRQ LEREKALLQH KNAEYQRKAD HEADKKRNLE NDVNSLKDQL
550 560 570 580 590 600
EDLKKRNQNS QISTEKVNQL QRQLDETNAL LRTESDTAAR LRKTQAESSK QIQQLESNNR
610 620 630 640 650 660
DLQDKNCLLE TAKLKLEKDF INLQSVLESE RRDRTHGSEI INDLQGRISG LEEDLKNGKI
670 680 690 700 710 720
LLTKVEMEKR QLQERFTDLE KEKNNMEIDM TYQLKVIQQS LEQEEAEHKA TKARLADKNK
730 740 750 760 770 780
IYESIEEAKS EAMKEMEKKL LEERTLKQKV ENLLLEAEKR CSILDCDLKQ SQQKINELLK
790 800 810 820 830 840
QKDVLNEDVR NLTLKIEQET QKRCLTQNDL KMQTQQVNTL KMSEKQLKQE NNHLMEMKMS
850 860 870 880 890 900
LEKQNAELRK ERQDADGQMK ELQDQLEAEQ YFSTLYKTQV RELKEECEEK TKLCKELQQK
910 920 930 940 950 960
KQELQDERDS LAAQLEITLT KADSEQLARS IAEEQYSDLE KEKIMKELEI KEMMARHKQE
970 980 990 1000 1010 1020
LTEKDTTIAS LEETNRTLTS DVANLANEKE ELNNKLKDAQ EQLSRLKDEE ISAAAIKAQF
1030 1040 1050 1060 1070 1080
EKQLLTERTL KTQAVNKLAE IMNRKEPVKR GNDTDMRRKE KENRKLHMEL KSEREKLTQQ
1090 1100 1110 1120 1130 1140
MIKYQKELNE MQAQIAEESQ IRIELQMTLD SKDSDIEQLR SQLQALHIGL DSSSIGSGPG
1150 1160 1170 1180 1190 1200
DAEADDGFPE SRLEGWLSLP VRNNTKKFGW VKKYVIVSSK KILFYDSEQD KEQSNPYMVL
1210 1220 1230 1240 1250 1260
DIDKLFHVRP VTQTDVYRAD AKEIPRIFQI LYANEGESKK EQEFPVEPVG EKSNCICHKG
1270 1280 1290 1300 1310 1320
HEFIPTLYHF PTNCEACMKP LWHMFKPPPA LECRRCHIKC HKDHMDKKEE IIAPCKVYYD
1330 1340 1350 1360 1370 1380
ISSAKNLLLL ANSTEEQQKW VSRLVKKIPK KPPAPDPFAR SSPRTSMKIQ QNQSIRRPSR
QLAANKPS