G5EFQ4

EV
Gene name
klp-6 (CELE_R144.1, R144.1)
Protein name
Kinesin-like protein
Names
Species
Caenorhabditis elegans
KEGG Pathway
cel:CELE_R144.1
EC number
Protein Class
STAR-RELATED LIPID TRANSFER PROTEIN 9 (PTHR47117)

Descriptions

KLP-6's autoinhibition is mediated by a comprehensive interaction between its N-terminal motor domain and multiple coiled-coil domains. This configuration restricts the motor domain's flexibility, hindering its ability to engage with microtubules efficiently. KLP-6 autoinhibition involves a multilevel-lockdown mechanism where internal coiled-coil segments and domains cooperate to lock down the neck and motor domains, consequently blocking nucleotide access and hindering microtubule binding. This intricate process effectively suppresses motor activity.

Autoinhibitory domains (AIDs)

395-446 (Coiled-coil 1, CC1 domain) EV

Target domain

368-394 (Neck coil); 1-357 (Motor domain)

Relief mechanism

Partner binding

Assay

Structural analysis, Mutagenesis experiment

447-842 (Coiled-coil 2 and internal domains, FHA, MBS, MATH domains) EV

Target domain

1-357 (Motor domain)

Relief mechanism

Partner binding

Assay

Structural analysis, Mutagenesis experiment

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for G5EFQ4

Entry ID Method Resolution Chain Position Source
7WRG X-ray 316 A A/B 1-928 PDB

No variants for G5EFQ4

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for G5EFQ4

No associated diseases with G5EFQ4

4 regional properties for G5EFQ4

Type Name Position InterPro Accession
domain Forkhead-associated (FHA) domain 483 - 542 IPR000253
domain Kinesin motor domain 4 - 366 IPR001752
conserved_site Kinesin motor domain, conserved site 251 - 262 IPR019821
domain Kinesin-like KIF1-type 659 - 699 IPR022140

Functions

Description
EC Number
Subcellular Localization
PANTHER Family PTHR47117 STAR-RELATED LIPID TRANSFER PROTEIN 9
PANTHER Subfamily PTHR47117:SF3 KINESIN FAMILY MEMBER 14-LIKE
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
axon The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter.
dendrite A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body.
kinesin complex Any complex that includes a dimer of molecules from the kinesin superfamily, a group of related proteins that contain an extended region of predicted alpha-helical coiled coil in the main chain that likely produces dimerization. The native complexes of several kinesin family members have also been shown to contain additional peptides, often designated light chains as all of the noncatalytic subunits that are currently known are smaller than the chain that contains the motor unit. Kinesin complexes generally possess a force-generating enzymatic activity, or motor, which converts the free energy of the gamma phosphate bond of ATP into mechanical work.
microtubule Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle.
mitochondrial membrane Either of the lipid bilayers that surround the mitochondrion and form the mitochondrial envelope.
neuronal cell body The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites.
non-motile cilium A cilium which may have a variable array of axonemal microtubules but does not contain molecular motors.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.
microtubule motor activity A motor activity that generates movement along a microtubule, driven by ATP hydrolysis.
RNA binding Binding to an RNA molecule or a portion thereof.

2 GO annotations of biological process

Name Definition
cytoskeleton-dependent intracellular transport The directed movement of substances along cytoskeletal fibers such as microfilaments or microtubules within a cell.
microtubule-based movement A microtubule-based process that results in the movement of organelles, other microtubules, or other cellular components. Examples include motor-driven movement along microtubules and movement driven by polymerization or depolymerization of microtubules.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
O43896 KIF1C Kinesin-like protein KIF1C Homo sapiens (Human) SS
P34540 unc-116 Kinesin heavy chain Caenorhabditis elegans SS
10 20 30 40 50 60
MGKGDSIIVA VRVRPFNDRE KTRNCKLVIE MPDEETTVIR DPKTNDEKRF TYDHSYWSHD
70 80 90 100 110 120
GFSEKKNGYL EPTDPHYADQ RRVFEDLGRG VLANAWAGYN CSLFAYGQTG SGKSYSIVGF
130 140 150 160 170 180
KNNKGIVPIV CEELFKQIAD NKKKNMQFEV FVSMMEIYCE KVRDLLSSTP PPKGGLKVRE
190 200 210 220 230 240
HPKNGFYVEN LTTVPVNSFK EIEAKIEEGT KSRTIAATQM NATSSRAHTI VKITFNQKSS
250 260 270 280 290 300
KQAGGTSMKK SEINLVDLAG SERQSAAGTE GDRLKEGIVI NQSLTTLGRV IKALHDSQKA
310 320 330 340 350 360
KSGKKTQIPY RDSVLTCLLK NALGGNSKTI MIAAISPADI NFEETLSTLR FADRAKSIKT
370 380 390 400 410 420
NAVVNENQTE RALRELREEN LRLQSQIQGG TAGDASNEEI EKLRRQLAEN QKEMEEMEKS
430 440 450 460 470 480
WQQKIAEEAA KHASGASEKV EMEAKKKKMC HLWNLNEDPA LTNVIVHFIP VGESVVGNKP
490 500 510 520 530 540
TSSGNFIQMS GLSILPQHVT LKNDGNNQIH LSPCSEDLDI FINGKPVHGE TQLQQNDRVF
550 560 570 580 590 600
FGGNHLYVFN NPTKKGIRTD ITYENAQAEI AQNHAAALGN RGLGGGSKRD LILEEELMST
610 620 630 640 650 660
LPLVQRANAM ATELGRNVKF EIVLVSPEMR GLTSGLTEIW VKVHNISEDT YFLWEKSRFM
670 680 690 700 710 720
NRYYGMQEMY EAKQDGSEDW NMPKERDPFY EPPDSPVFIA SSVVFLQSLA YLIDVEEQFP
730 740 750 760 770 780
IVDLSGQEIG LLTVGLSPCS TTGKELRGEY VEDPDQLIGK NIAFKVKVIS AVGLPRRILK
790 800 810 820 830 840
SNCKYRFFGS KKMTTTATVS GNTPAYGHEE TFQFKPVTKE VADYLANSNL YITFWGTQRP
850 860 870 880 890 900
RGASSRKNSI STIGSNEARE GPNKAKRVER LVHQAKTSEN RNISVKALET VLKGVDDNEN
910 920
RKTRKQSMKK AGSTTIKSRS GSKKPKAK