F1MHT9

SS
Gene name
NLRC4 (IPAF)
Protein name
NLR family CARD domain-containing protein 4
Names
Ice protease-activating factor, Ipaf
Species
Bos taurus (Bovine)
KEGG Pathway
bta:512480
EC number
Protein Class

Descriptions

Nucleotide-binding and oligomerization domain-like receptor (NLR) proteins oligomerize into multiprotein complexes termed inflammasomes when activated. NLRs constitute a crucial component of the cytosolic immunosurveillance system of mammals by detecting the signature components of pathogens and consequently triggering immune responses. The structure of NLRC2 (NLR family CARD domain-containing protein 4) is in a closed form. The adenosine diphosphate-mediated interaction between the central nucleotide-binding domain (NBD) and the winged-helix domain (WHD) was critical for stabilizing the closed conformation. In addition, the helical domain HD2 repressively contacted a conserved and functionally important α-helix of the NBD. The C-terminal leucine-rich repeat (LRR) domain is positioned to sterically occlude one side of the NBD domain and consequently sequester NLRC4 in monomeric state. Therefore, disruption of ADP-mediated NBD-WHD or NBD-HD2/NBD-LRR interactions resulted in constitutive activation of NLRC4.

Autoinhibitory domains (AIDs)

356-463 (WHD) SS

Target domain

95-298 (Nucleotide-binding domain, NBD)

Relief mechanism

Others

Assay

578-1014 (LRR) SS

Target domain

95-298 (Nucleotide-binding domain, NBD)

Relief mechanism

Ligand binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for F1MHT9

Entry ID Method Resolution Chain Position Source
AF-F1MHT9-F1 Predicted AlphaFoldDB

No variants for F1MHT9

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for F1MHT9

No associated diseases with F1MHT9

3 regional properties for F1MHT9

Type Name Position InterPro Accession
domain Pectinesterase, catalytic 278 - 571 IPR000070
domain Pectinesterase inhibitor domain 42 - 195 IPR006501
active_site Pectinesterase, Asp active site 422 - 431 IPR033131

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm, cytosol
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
IPAF inflammasome complex An inflammasome complex that consists of three components, IPAF, NAIP and caspase-1, and includes among its functions the sensing of flagellin derived from Legionella pneumophila, Salmonella typhimurium, Pseudomonas aeruginosa and Shigella flexneri.

1 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.

10 GO annotations of biological process

Name Definition
activation of innate immune response Any process that initiates an innate immune response. Innate immune responses are defense responses mediated by germline encoded components that directly recognize components of potential pathogens. Examples of this process include activation of the hypersensitive response of Arabidopsis thaliana and activation of any NOD or TLR signaling pathway in vertebrate species.
apoptotic process A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
defense response to bacterium Reactions triggered in response to the presence of a bacterium that act to protect the cell or organism.
detection of bacterium The series of events in which a stimulus from a bacterium is received and converted into a molecular signal.
inflammatory response The immediate defensive reaction (by vertebrate tissue) to infection or injury caused by chemical or physical agents. The process is characterized by local vasodilation, extravasation of plasma into intercellular spaces and accumulation of white blood cells and macrophages.
innate immune response Innate immune responses are defense responses mediated by germline encoded components that directly recognize components of potential pathogens.
positive regulation of interleukin-1 beta production Any process that activates or increases the frequency, rate, or extent of interleukin-1 beta production.
protein homooligomerization The process of creating protein oligomers, compounds composed of a small number, usually between three and ten, of identical component monomers. Oligomers may be formed by the polymerization of a number of monomers or the depolymerization of a large protein polymer.
pyroptosis A caspase-1-dependent cell death subroutine that is associated with the generation of pyrogenic mediators such as IL-1beta and IL-18.
regulation of apoptotic process Any process that modulates the occurrence or rate of cell death by apoptotic process.

22 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q9NPP4 NLRC4 NLR family CARD domain-containing protein 4 Homo sapiens (Human) SS
Q3UP24 Nlrc4 NLR family CARD domain-containing protein 4 Mus musculus (Mouse) EV
F1M649 Nlrc4 NLR family CARD domain-containing protein 4 Rattus norvegicus (Rat) SS
Q39214 RPM1 Disease resistance protein RPM1 Arabidopsis thaliana (Mouse-ear cress) PR
Q8W3K3 At1g58400 Putative disease resistance protein At1g58400 Arabidopsis thaliana (Mouse-ear cress) PR
Q9FJB5 RPP8L3 Disease resistance RPP8-like protein 3 Arabidopsis thaliana (Mouse-ear cress) PR
P60838 SUMM2 Disease resistance protein SUMM2 Arabidopsis thaliana (Mouse-ear cress) PR
Q9LRR4 RPPL1 Putative disease resistance RPP13-like protein 1 Arabidopsis thaliana (Mouse-ear cress) PR
Q9T048 At4g27190 Disease resistance protein At4g27190 Arabidopsis thaliana (Mouse-ear cress) PR
O64973 RPS5 Disease resistance protein RPS5 Arabidopsis thaliana (Mouse-ear cress) PR
Q38834 RPP13L4 Disease resistance RPP13-like protein 4 Arabidopsis thaliana (Mouse-ear cress) PR
Q9C646 RXW24L Probable disease resistance protein RXW24L Arabidopsis thaliana (Mouse-ear cress) PR
O22727 At1g61190 Probable disease resistance protein At1g61190 Arabidopsis thaliana (Mouse-ear cress) PR
Q9FKZ0 At5g66910 Probable disease resistance protein At5g66910 Arabidopsis thaliana (Mouse-ear cress) PR
Q9XIF0 At1g59780 Putative disease resistance protein At1g59780 Arabidopsis thaliana (Mouse-ear cress) PR
P0C8S1 RPP8L2 Probable disease resistance RPP8-like protein 2 Arabidopsis thaliana (Mouse-ear cress) PR
F4J339 RPP1 Probable disease resistance protein RPP1 Arabidopsis thaliana (Mouse-ear cress) PR
O64790 At1g61300 Probable disease resistance protein At1g61300 Arabidopsis thaliana (Mouse-ear cress) PR
Q940K0 UNI Disease resistance protein UNI Arabidopsis thaliana (Mouse-ear cress) PR
Q9XGM3 RPS4 Disease resistance protein RPS4 Arabidopsis thaliana (Mouse-ear cress) PR
O64789 At1g61310 Probable disease resistance protein At1g61310 Arabidopsis thaliana (Mouse-ear cress) PR
O23317 At4g14610 Probable disease resistance protein At4g14610 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MNFIKENSQV LIQRMGMTVI KQILDELFVW NVMNYEEVNV ICGEKFEQDA ARGVIHMILK
70 80 90 100 110 120
KGSEACNLFL KSLEKWNYPL FQELHGLSLF HQMSEEDLDD LAQELKYFYQ SPSFLNFYPL
130 140 150 160 170 180
GEDIDIMFNL KSTFTEPVLW KKDQHHHRLE QLTLSGLLDT LQSPCIIEGE SGKGKSTLLQ
190 200 210 220 230 240
RIAMLWASGE CQALTKFKLV FFLRLSRAQG GLFETLSDQL LDIPDVISKQ TFMARLLKLR
250 260 270 280 290 300
QRVLFLLDGY NEFKAQNCPE IEALIKENHR FKNMVIVTTT TESLRHIRQF GALIAEVGDM
310 320 330 340 350 360
TESSAQALIQ EVLRKEFAED LLLQIQKSRC LRNLMKTPLF VVITCAIQMG KSEFHSHTQT
370 380 390 400 410 420
TLFCTFYDLL INKNRHKRKG LAPSEVTQSL DHCGDLALEG VFSRRFDFEP DDLSNVNEDV
430 440 450 460 470 480
LLTTGLLCKY TAQRFKPKYK FFHQSFQEYT AGRRLSSLLT SGEPAEVTKG NGHLQKMVSI
490 500 510 520 530 540
SDITSKYSNL LLYTCGSSAE ATRTVLKHLS SVYQHGSLLG LSVTKRPLWR QESMQNMKST
550 560 570 580 590 600
TVQEILKAIN INSFTECGIN LFHESISTSS LSKEFEDFFR GKSLYINSEN IPDYLFDFFE
610 620 630 640 650 660
DLPNCASALD FVKLDFYGGA VRDISGNQDQ EFSGTYIPSR AVSLFFNWKQ EFKTLDVTLR
670 680 690 700 710 720
DFCKLSKKDI KYLEKIFSSA TSLRLHIKRC VGMAGSLSSV LSTCKNIHSL IVEASPLTLE
730 740 750 760 770 780
DEQHITSVTN LQTLGVHDLQ IQRLPGGLTD NLGNLKNLMK LILDNIQMNE EDALKLAEGL
790 800 810 820 830 840
TNLKKMCLLR LTHLSDIGEG MDYIVKSLSA EPCDLKEIQL VSCCLSGNAV KTLAQNLHNL
850 860 870 880 890 900
ARLSILDLSE NHLEKDGKEA LQQLIDRLHI LEQLTVLMLP WCGDVRVSLA RLLEQLERVP
910 920 930 940 950 960
QLVKLGLKNW RLTDAEIRIL GVFFEKNPLE NFQQLDLAGN CVSSDGWLAF MSGFENLKEL
970 980 990 1000 1010
VFFDFSTKGL LPDASLVRKL SHVLSKLTFL QEVQLVGWQL DDDDVSVLKG AFKLVIA