F1M7Y5

SS
Gene name
Prkci (Pkcl)
Protein name
Protein kinase C iota type
Names
EC 2.7.11.13 , Atypical protein kinase C-lambda/iota , aPKC-lambda/iota , nPKC-iota
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:84006
EC number
2.7.11.13: Protein-serine/threonine kinases
Protein Class

Descriptions

PRKCI, an atypical PKC (aPKC) isoform, is an important kinase in multiple cellular processes and regulated by the phosphatidylinositol 3-kinase product phosphatidylinositol 3,4,5-(PO4)3 (PIP3). In aPKC molecule, the arginine motif (123-140) in the pseudosubstrate region on the regulatory domain binds to acidic residues in the substrate-binding region on the catalytic domain, leading to the off state of aPKCs. The autoinhibition of aPKC is released by binding with the acidic ligand PIP3 and consequently exposing the substrate-binding site.

Autoinhibitory domains (AIDs)

123-140 (Pseudosubstrate region) SS

Target domain

233-596 (Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C iota)

Relief mechanism

Ligand binding

Assay

Accessory elements

395-418 (Activation loop from InterPro)

Target domain

233-596 (Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C iota)

Relief mechanism

Assay

395-418 (Activation loop from InterPro)

Target domain

233-596 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for F1M7Y5

Entry ID Method Resolution Chain Position Source
AF-F1M7Y5-F1 Predicted AlphaFoldDB

No variants for F1M7Y5

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for F1M7Y5

No associated diseases with F1M7Y5

14 regional properties for F1M7Y5

Type Name Position InterPro Accession
domain PB1 domain 25 - 107 IPR000270
domain Protein kinase domain 254 - 522 IPR000719
domain AGC-kinase, C-terminal 523 - 594 IPR000961
domain Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 140 - 192 IPR002219
active_site Serine/threonine-protein kinase, active site 374 - 386 IPR008271
binding_site Protein kinase, ATP binding site 260 - 287 IPR017441
domain Protein kinase, C-terminal 546 - 584 IPR017892
domain Diacylglycerol/phorbol-ester binding 138 - 152 IPR020454-1
domain Diacylglycerol/phorbol-ester binding 154 - 163 IPR020454-2
domain Diacylglycerol/phorbol-ester binding 167 - 178 IPR020454-3
domain Diacylglycerol/phorbol-ester binding 179 - 191 IPR020454-4
domain Atypical protein kinase C iota type, catalytic domain 233 - 596 IPR034661
domain Protein kinase C, PB1 domain 26 - 108 IPR034877
domain PB1-like domain 25 - 108 IPR053793

Functions

Description
EC Number 2.7.11.13 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Membrane
  • Endosome
  • Nucleus
  • Transported into the endosome through interaction with SQSTM1/p62
  • After phosphorylation by SRC, transported into the nucleus through interaction with KPNB1
  • Colocalizes with CDK7 in the cytoplasm and nucleus
  • Transported to vesicular tubular clusters (VTCs) through interaction with RAB2A
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

15 GO annotations of cellular component

Name Definition
apical part of cell The region of a polarized cell that forms a tip or is distal to a base. For example, in a polarized epithelial cell, the apical region has an exposed surface and lies opposite to the basal lamina that separates the epithelium from other tissue.
apical plasma membrane The region of the plasma membrane located at the apical end of the cell.
brush border The dense covering of microvilli on the apical surface of an epithelial cell in tissues such as the intestine, kidney, and choroid plexus; the microvilli aid absorption by increasing the surface area of the cell.
cell leading edge The area of a motile cell closest to the direction of movement.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
endosome A vacuole to which materials ingested by endocytosis are delivered.
glutamatergic synapse A synapse that uses glutamate as a neurotransmitter.
Golgi membrane The lipid bilayer surrounding any of the compartments of the Golgi apparatus.
intercellular bridge A direct connection between the cytoplasm of two cells that is formed following the completion of cleavage furrow ingression during cell division. They are usually present only briefly prior to completion of cytokinesis. However, in some cases, such as the bridges between germ cells during their development, they become stabilised.
microtubule cytoskeleton The part of the cytoskeleton (the internal framework of a cell) composed of microtubules and associated proteins.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
PAR polarity complex A protein kinase complex that is required for the establishment of a cell polarity axis during the cell division cycle. Binds directly to activated CDC42 GTPase and is required for orchestrating a cellular gradient of CDC42. In S. cerevisiae components are
Schaffer collateral - CA1 synapse A synapse between the Schaffer collateral axon of a CA3 pyramidal cell and a CA1 pyramidal cell.
Schmidt-Lanterman incisure Regions within compact myelin in which the cytoplasmic faces of the enveloping myelin sheath are not tightly juxtaposed, and include cytoplasm from the cell responsible for making the myelin. Schmidt-Lanterman incisures occur in the compact myelin internode, while lateral loops are analogous structures found in the paranodal region adjacent to the nodes of Ranvier.

7 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
diacylglycerol-dependent serine/threonine kinase activity Catalysis of the reaction
metal ion binding Binding to a metal ion.
phospholipid binding Binding to a phospholipid, a class of lipids containing phosphoric acid as a mono- or diester.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

21 GO annotations of biological process

Name Definition
actin filament organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments. Includes processes that control the spatial distribution of actin filaments, such as organizing filaments into meshworks, bundles, or other structures, as by cross-linking.
cell migration The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues.
cell-cell junction organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a cell-cell junction. A cell-cell junction is a specialized region of connection between two cells.
cellular response to insulin stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an insulin stimulus. Insulin is a polypeptide hormone produced by the islets of Langerhans of the pancreas in mammals, and by the homologous organs of other organisms.
establishment of apical/basal cell polarity The specification and formation of the polarity of a cell along its apical/basal axis.
establishment or maintenance of epithelial cell apical/basal polarity Any cellular process that results in the specification, formation or maintenance of the apicobasal polarity of an epithelial cell.
eye photoreceptor cell development Development of a photoreceptor, a sensory cell in the eye that reacts to the presence of light. They usually contain a pigment that undergoes a chemical change when light is absorbed, thus stimulating a nerve.
Golgi vesicle budding The evagination of the Golgi membrane, resulting in formation of a vesicle.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
negative regulation of glial cell apoptotic process Any process that stops, prevents, or reduces the frequency, rate, or extent of glial cell apoptotic process.
negative regulation of neuron apoptotic process Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process in neurons.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
positive regulation of endothelial cell apoptotic process Any process that activates or increases the frequency, rate or extent of endothelial cell apoptotic process.
positive regulation of glial cell proliferation Any process that activates or increases the rate or extent of glial cell proliferation.
positive regulation of glucose import Any process that activates or increases the frequency, rate or extent of the import of the hexose monosaccharide glucose into a cell or organelle.
positive regulation of neuron projection development Any process that increases the rate, frequency or extent of neuron projection development. Neuron projection development is the process whose specific outcome is the progression of a neuron projection over time, from its formation to the mature structure. A neuron projection is any process extending from a neural cell, such as axons or dendrites (collectively called neurites).
positive regulation of NF-kappaB transcription factor activity Any process that activates or increases the frequency, rate or extent of activity of the transcription factor NF-kappaB.
positive regulation of protein localization to plasma membrane Any process that activates or increases the frequency, rate or extent of protein localization to plasma membrane.
regulation of postsynaptic membrane neurotransmitter receptor levels Any process that regulates the the local concentration of neurotransmitter receptor at the postsynaptic membrane.
response to interleukin-1 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an interleukin-1 stimulus.
response to peptide hormone Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a peptide hormone stimulus. A peptide hormone is any of a class of peptides that are secreted into the blood stream and have endocrine functions in living animals.

21 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P24583 PKC1 Protein kinase C-like 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SS
A1Z9X0 aPKC Atypical protein kinase C Drosophila melanogaster (Fruit fly) SS
Q05513 PRKCZ Protein kinase C zeta type Homo sapiens (Human) SS
P41743 PRKCI Protein kinase C iota type Homo sapiens (Human) EV
Q02956 Prkcz Protein kinase C zeta type Mus musculus (Mouse) SS
Q62074 Prkci Protein kinase C iota type Mus musculus (Mouse) SS
P47196 Akt1 RAC-alpha serine/threonine-protein kinase Rattus norvegicus (Rat) PR
O55173 Pdpk1 3-phosphoinositide-dependent protein kinase 1 Rattus norvegicus (Rat) SS
P09217 Prkcz Protein kinase C zeta type Rattus norvegicus (Rat) SS
Q63433 Pkn1 Serine/threonine-protein kinase N1 Rattus norvegicus (Rat) SS
Q64617 Prkch Protein kinase C eta type Rattus norvegicus (Rat) PR
P09215 Prkcd Protein kinase C delta type Rattus norvegicus (Rat) PR
P63319 Prkcg Protein kinase C gamma type Rattus norvegicus (Rat) SS
P47197 Akt2 RAC-beta serine/threonine-protein kinase Rattus norvegicus (Rat) SS
Q63484 Akt3 RAC-gamma serine/threonine-protein kinase Rattus norvegicus (Rat) PR
O08874 Pkn2 Serine/threonine-protein kinase N2 Rattus norvegicus (Rat) SS
P09216 Prkce Protein kinase C epsilon type Rattus norvegicus (Rat) PR
P05696 Prkca Protein kinase C alpha type Rattus norvegicus (Rat) SS
P68403 Prkcb Protein kinase C beta type Rattus norvegicus (Rat) SS
Q19266 pkc-3 Protein kinase C-like 3 Caenorhabditis elegans SS
Q90XF2 prkci Protein kinase C iota type Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MPTQRDSSTM SHTVACGGGG DHSHQVRVKA YYRGDIMITH FEPSISFEGL CSEVRDMCSF
70 80 90 100 110 120
DNEQPFTMKW IDEEGDPCTV SSQLELEEAF RLYELNKDSE LLIHVFPCVP ERPGMPCPGE
130 140 150 160 170 180
DKSIYRRGAR RWRKLYCANG HTFQAKRFNR RAHCAICTDR IWGLGRQGYK CINCKLLVHK
190 200 210 220 230 240
KCHKLVTIEC GRHSLPPEPM MPMDQSSMHP DHTQTVIPYN PSSHESLDQV GEEKEAMNTR
250 260 270 280 290 300
ESGKASSSLG LQDFDLLRVI GRGSYAKVLL VRLKKTDRIY AMKVVKKELV NDDEDIDWVQ
310 320 330 340 350 360
TEKHVFEQAS NHPFLVGLHS CFQTESRLFF VIEYVNGGDL MFHMQRQRKL PEEHARFYSA
370 380 390 400 410 420
EISLALNYLH ERGIIYRDLK LDNVLLDSEG HIKLTDYGMC KEGLRPGDTT STFCGTPNYI
430 440 450 460 470 480
APEILRGEDY GFSVDWWALG VLMFEMMAGR SPFDIVGSSD NPDQNTEDYL FQVILEKQIR
490 500 510 520 530 540
IPRSLSVKAA SVLKSFLNKD PKERLGCHPQ TGFADIQGHP FFRNVDWDMM EQKQVVPPFK
550 560 570 580 590
PNISGEFGLD NFDSQFTNEP VQLTPDDDDI VRKIDQSEFE GFEYINPLLM SAEECV