F1M5N7

SS
Gene name
Kif21b
Protein name
Kinesin-like protein KIF21B
Names
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:289397
EC number
Protein Class

Descriptions

KIF21B, a microtubule(MT)-plus-end directed kinesin-4 motor protein, is mainly expressed in spleen, testes and central nervous tissues, particularly enriched in neurons and contains an N-terminal motor domain followed by several coiled coils and a C-terminal WD40 domain. Within neurons, KIF21B promotes intracellular transport through its N-terminal processive motor activity and influences MT dynamics through distinct MT binding domains. KIF21B positively regulates MTs dynamicity in dendrites by favoring MT growth and catastrophes and also acts as a MT pausing factor by accumulating at the MT-plus ends. These KIF21B functions can be modulated through an autoinhibitory interaction between the N-terminal motor domain and an internal regulatory coiled-coil region (rCC). Mutations within the coiled-coil region are associated with autoinhibitory release, which leads to failure of the fine-tuning of KIF21B activity, resulting in defects in proper neuronal migration and axonal growth.

Autoinhibitory domains (AIDs)

925-1006 (Regulatory coiled-coil domain) SS

Target domain

6-379 (Kinesin motor domain)

Relief mechanism

Partner binding, Others

Assay

1042-1072 (3rd coiled-coil stalk domain) SS

Target domain

8-371 (Motor domain)

Relief mechanism

Others

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for F1M5N7

Entry ID Method Resolution Chain Position Source
AF-F1M5N7-F1 Predicted AlphaFoldDB

3 variants for F1M5N7

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3322417770 533 S>N No EVA
rs3322218112 838 P>T No EVA
rs107050503 1461 T>I No EVA

No associated diseases with F1M5N7

3 regional properties for F1M5N7

Type Name Position InterPro Accession
domain Protein kinase domain 23 - 277 IPR000719
active_site Serine/threonine-protein kinase, active site 139 - 151 IPR008271
binding_site Protein kinase, ATP binding site 29 - 56 IPR017441

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm, cytoskeleton
  • Cell projection, dendrite
  • Cell projection, growth cone
  • Cell projection, axon
  • Cytoplasmic vesicle
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cytoplasmic vesicle A vesicle found in the cytoplasm of a cell.
dendrite A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body.
growth cone The migrating motile tip of a growing neuron projection, where actin accumulates, and the actin cytoskeleton is the most dynamic.
kinesin complex Any complex that includes a dimer of molecules from the kinesin superfamily, a group of related proteins that contain an extended region of predicted alpha-helical coiled coil in the main chain that likely produces dimerization. The native complexes of several kinesin family members have also been shown to contain additional peptides, often designated light chains as all of the noncatalytic subunits that are currently known are smaller than the chain that contains the motor unit. Kinesin complexes generally possess a force-generating enzymatic activity, or motor, which converts the free energy of the gamma phosphate bond of ATP into mechanical work.
microtubule Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.
microtubule motor activity A motor activity that generates movement along a microtubule, driven by ATP hydrolysis.

5 GO annotations of biological process

Name Definition
brain development The process whose specific outcome is the progression of the brain over time, from its formation to the mature structure. Brain development begins with patterning events in the neural tube and ends with the mature structure that is the center of thought and emotion. The brain is responsible for the coordination and control of bodily activities and the interpretation of information from the senses (sight, hearing, smell, etc.).
corpus callosum development The process whose specific outcome is the progression of the corpus callosum over time, from its formation to the mature structure. The corpus callosum is a thick bundle of nerve fibers comprising a commissural plate connecting the two cerebral hemispheres. It consists of contralateral axon projections that provide communication between the right and left cerebral hemispheres.
microtubule-based movement A microtubule-based process that results in the movement of organelles, other microtubules, or other cellular components. Examples include motor-driven movement along microtubules and movement driven by polymerization or depolymerization of microtubules.
mitotic spindle organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the microtubule spindle during a mitotic cell cycle.
spindle elongation The cell cycle process in which the distance is lengthened between poles of the spindle.

15 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q86VH2 KIF27 Kinesin-like protein KIF27 Homo sapiens (Human) SS
Q2M1P5 KIF7 Kinesin-like protein KIF7 Homo sapiens (Human) EV
O95239 KIF4A Chromosome-associated kinesin KIF4A Homo sapiens (Human) SS
Q7Z4S6 KIF21A Kinesin-like protein KIF21A Homo sapiens (Human) EV
Q2VIQ3 KIF4B Chromosome-associated kinesin KIF4B Homo sapiens (Human) SS
O75037 KIF21B Kinesin-like protein KIF21B Homo sapiens (Human) EV
Q7M6Z4 Kif27 Kinesin-like protein KIF27 Mus musculus (Mouse) SS
B7ZNG0 Kif7 Kinesin-like protein KIF7 Mus musculus (Mouse) SS
Q9QXL2 Kif21a Kinesin-like protein KIF21A Mus musculus (Mouse) EV SS
Q9QXL1 Kif21b Kinesin-like protein KIF21B Mus musculus (Mouse) SS
Q7M6Z5 Kif27 Kinesin-like protein KIF27 Rattus norvegicus (Rat) SS
Q2PQA9 Kif5b Kinesin-1 heavy chain Rattus norvegicus (Rat) SS
Q6QLM7 Kif5a Kinesin heavy chain isoform 5A Rattus norvegicus (Rat) SS
Q9WV63 Kif2a Kinesin-like protein KIF2A Rattus norvegicus (Rat) PR
Q58G59 kif7 Kinesin-like protein kif7 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MAGQGDCCVK VAVRIRPQLS KEKIEGCHIC TSVTPGEPQV LLGKDKAFTY DFVFDLDTWQ
70 80 90 100 110 120
EQIYSTCVSK LIEGCFEGYN ATVLAYGQTG AGKTYTMGTG FDTVTSEEEQ GIIPRAIAHL
130 140 150 160 170 180
FRGIDERKRR AQEQGVTGPE FKVSAQFLEL YNEEILDLFD STRDPDARHR RSNIKIHEDA
190 200 210 220 230 240
NGGIYTTGVT SRLINSQEEL IQCLKQGALS RTTASTQMNV QSSRSHAIFT IHLCQMRVCA
250 260 270 280 290 300
QPDLVNETVT GLPDGTAPTG TEYETLTAKF HFVDLAGSER LKRTGATGER AKEGISINCG
310 320 330 340 350 360
LLALGNVISA LGDQSKKVVH VPYRDSKLTR LLQDSLGGNS QTIMIACVSP SDRDFMETLN
370 380 390 400 410 420
TLKYANRARN IKNKVVVNQD KTSQQISALR AEIARLQMEL MEYKAGKRVI GEDGAEGYSD
430 440 450 460 470 480
LFRENAMLQK ENGALRLRVK AMQEAIDAIN NRVTQLMSQE ANLLLAKAGD GNEAIGALIQ
490 500 510 520 530 540
NYIREIEELR TKLLESEAMN ESLRRSLSRA SARNPYSLGA SPAGPAFGGS PASSMEDASE
550 560 570 580 590 600
VIRRAKQDLE RLKKKEVRQR RKSPEKEAFK KRAKLQQENS EETDENEAEE EEEERDESGC
610 620 630 640 650 660
EEEEDEDSGS EESLVDSDSD PEEKEVNFQA DLADLTCEIE IKQKLIDELE NSQRRLQTLK
670 680 690 700 710 720
HQYEEKLILL QNKIRDTQLE RDRVLQNLST MECYTEEKAN KIKADYEKRL REMNRDLQKL
730 740 750 760 770 780
QAAQKEHARL LKNQSRYERE LKKLQTEVAE MKKAKVALMK QMREEQQRRR LVETKRNREI
790 800 810 820 830 840
AQLKKEQRRQ EFQIRALESQ KRQQEIVLRR KTQEVSALRR LAKPMSERVA GRVGLKPPNM
850 860 870 880 890 900
DSGAEVSAST TSSEAESGAR SVSSIVRQWN RKINHFLGDH PTATVNGARP ARKKFQKKGA
910 920 930 940 950 960
SQSFSKAARL KWQSLERRII DIVMQRMTIV NLEADMERLI KKREELFLLQ EALRRKRERL
970 980 990 1000 1010 1020
QAESPEEEKG LQELAEEIEV LAANIDYIND SITDCQATIM QLEETKEELD STDTSVVISS
1030 1040 1050 1060 1070 1080
CSLAEARLLL DNFLKASIDK GLQVAQKEAQ IRLLEGRLRQ TDMTGSSQNH LLLDALREKA
1090 1100 1110 1120 1130 1140
EAHPELQALI YNVQHENGYA STDEEVSEFS EGSFSQSFTM KGSTSHDDFK FKGEPKLSAQ
1150 1160 1170 1180 1190 1200
MKAVSAECLG PPLDSSTKNI TKSLASLVEI KEDGVGFSIR DPYYRDKVSR TVSLPTRGST
1210 1220 1230 1240 1250 1260
FPRQSRGATD TSPLTRRKSY DRGQPIRSTD IGFTPPSSPP TRPRNDRNVF SRLTSNQSQG
1270 1280 1290 1300 1310 1320
SALDKSDDSD SSLSEVLRGI ITPIGGAKGA RTAPLQCVSM AEGHTKPILC LDATDELLFT
1330 1340 1350 1360 1370 1380
GSKDRSCKMW NLVTGQEIAA LKGHPNNVVS IKYCSHSGLV FSVSSSYVKV WDIRDSAKCI
1390 1400 1410 1420 1430 1440
RTLTSSGQVI SGDACMATST RAITSSQGEH QINQMALSPS GTMLYVASGN AVRIWELNRF
1450 1460 1470 1480 1490 1500
QPIGKLTGHI GPVMCLTVTQ TSNQHDLVVT GSKDHYVKMF QLGDCVTGTI GPTHNFEPPH
1510 1520 1530 1540 1550 1560
YDGIECLAIQ GDILFSGSRD NGIKKWDLDQ QELIQQIPNA HKDWVCALAF VPGRPMLLSA
1570 1580 1590 1600 1610 1620
CRAGFIKVWN VDNFTPIGEI KGHDSPINAI CTNSKHIFTA SSDCRVKLWN YVPGLTPCLP
1630
RRVLAIKGRA TTLP