E1C656

SS
Gene name
HACE1 (RCJMB04_37k12)
Protein name
E3 ubiquitin-protein ligase HACE1
Names
EC 2.3.2.26 , HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 , HECT-type E3 ubiquitin transferase HACE1
Species
Gallus gallus (Chicken)
KEGG Pathway
gga:421788
EC number
2.3.2.26: Aminoacyltransferases
Protein Class

Descriptions

E3 ubiquitin-protein ligase HACE1 (HACE1) is a protein involved in Golgi membrane fusion and regulation of small GTPases. It plays a key role as a regulator of Golgi membrane dynamics during the cell cycles, recruiting to Golgi membrane by Rab proteins and regulating postmitotic Golgi membrane fusion. Autoinhibition of HACE1 is controlled by dimerization that induces a configuration inhibiting the ubiquitin transfer from E2 to substrate. This inhibitory state is maintained by specific interactions between the N-helix of one subunit and the small wing of the HECT N-lobe of the adjacent subunit.

Autoinhibitory domains (AIDs)

1-21 (N-helix) SS

Target domain

557-819 (N-lobe of HECT domain)

Relief mechanism

PTM, Partner binding, Ligand binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for E1C656

Entry ID Method Resolution Chain Position Source
AF-E1C656-F1 Predicted AlphaFoldDB

6 variants for E1C656

Variant ID(s) Position Change Description Diseaes Association Provenance
rs738198824 2 E>G No EVA
rs740199932 11 L>P No EVA
rs740483710 408 T>P No EVA
rs731676139 465 A>G No EVA
rs736115172 632 E>G No EVA
rs1058676565 888 L>S No EVA

No associated diseases with E1C656

3 regional properties for E1C656

Type Name Position InterPro Accession
domain HECT domain 587 - 942 IPR000569
repeat Ankyrin repeat 64 - 195 IPR002110-1
repeat Ankyrin repeat 196 - 257 IPR002110-2

Functions

Description
EC Number 2.3.2.26 Aminoacyltransferases
Subcellular Localization
  • Golgi apparatus, Golgi stack membrane
  • Cytoplasm
  • Endoplasmic reticulum
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
endoplasmic reticulum The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisternae. The ER takes two forms, rough (or granular), with ribosomes adhering to the outer surface, and smooth (with no ribosomes attached).
Golgi cisterna membrane The lipid bilayer surrounding any of the thin, flattened compartments that form the central portion of the Golgi complex.
Golgi membrane The lipid bilayer surrounding any of the compartments of the Golgi apparatus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

3 GO annotations of molecular function

Name Definition
small GTPase binding Binding to a small monomeric GTPase.
ubiquitin protein ligase activity Catalysis of the transfer of ubiquitin to a substrate protein via the reaction X-ubiquitin + S -> X + S-ubiquitin, where X is either an E2 or E3 enzyme, the X-ubiquitin linkage is a thioester bond, and the S-ubiquitin linkage is an amide bond
ubiquitin-protein transferase activity Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages.

8 GO annotations of biological process

Name Definition
cell cycle The progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events. Canonically, the cell cycle comprises the replication and segregation of genetic material followed by the division of the cell, but in endocycles or syncytial cells nuclear replication or nuclear division may not be followed by cell division.
Golgi organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the Golgi apparatus.
membrane fusion The membrane organization process that joins two lipid bilayers to form a single membrane.
protein K48-linked ubiquitination A protein ubiquitination process in which a polymer of ubiquitin, formed by linkages between lysine residues at position 48 of the ubiquitin monomers, is added to a protein. K48-linked ubiquitination targets the substrate protein for degradation.
protein polyubiquitination Addition of multiple ubiquitin groups to a protein, forming a ubiquitin chain.
protein ubiquitination The process in which one or more ubiquitin groups are added to a protein.
regulation of cell migration Any process that modulates the frequency, rate or extent of cell migration.
ubiquitin-dependent protein catabolic process The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of a ubiquitin group, or multiple ubiquitin groups, to the protein.

12 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
F1N6G5 HACE1 E3 ubiquitin-protein ligase HACE1 Bos taurus (Bovine) SS
P16157 ANK1 Ankyrin-1 Homo sapiens (Human) EV
Q12955 ANK3 Ankyrin-3 Homo sapiens (Human) SS
Q01484 ANK2 Ankyrin-2 Homo sapiens (Human) EV
Q8IYU2 HACE1 E3 ubiquitin-protein ligase HACE1 Homo sapiens (Human) EV
Q8C8R3 Ank2 Ankyrin-2 Mus musculus (Mouse) SS
Q02357 Ank1 Ankyrin-1 Mus musculus (Mouse) SS
Q3U0D9 Hace1 E3 ubiquitin-protein ligase HACE1 Mus musculus (Mouse) SS
O70511 Ank3 Ankyrin-3 Rattus norvegicus (Rat) EV
D3ZBM7 Hace1 E3 ubiquitin-protein ligase HACE1 Rattus norvegicus (Rat) SS
Q28BK1 hace1 E3 ubiquitin-protein ligase HACE1 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
F8W2M1 hace1 E3 ubiquitin-protein ligase HACE1 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MERAMEQLNR LTRSLRRART VELPDDNETA VYTLMPMVMA DQHRSVSELL SNSKFDVNYA
70 80 90 100 110 120
FGRVKRSLLH IAANCGSVEC LVLLLKKGAN PNYQDISGCT PLHLAARNGQ KKCMSKLLEY
130 140 150 160 170 180
SADVNICNNE GLTAIHWLAV NGRTELLHDL VQHVSNVDVE DAMGQTALHV ACQNGHKTTV
190 200 210 220 230 240
QCLLDSGADI NRPNVSGATP LYFACSHGQR DTAQILLMRG AKYLPDKNGI TPLDLCVQGG
250 260 270 280 290 300
YGETCEVLIQ YHPRLFQTII QMTQNEDLRE NMLRQVLEHL SQQSESQYLK ILTSLAEVAT
310 320 330 340 350 360
TNGHKLLSLS SNYEAQMKSL LRIVRIFCHV FRIGPSSPSN GNDMGYNGNK TPRSQVFKVR
370 380 390 400 410 420
KVYDVVRKID VKEMNFTKHA FINQTSHEQE PLELLWHSLD EWLVLIATEL MKNKRDSANI
430 440 450 460 470 480
TSILLKQKGP DHQDATPTPS FAAAGTESRK ELSTDTGDSK TYEVAGKQEA YADCQDVISM
490 500 510 520 530 540
TANRLSAVIQ AFYMCCSCQM PQGMTSPRFI EFVCKHDDVL KCFVNRNPKI IFDHFHFLLE
550 560 570 580 590 600
CPELMSRFMH IIKAQPFKDR CEWFYEHLHS GQPDSDMVHR PVNENDILLV HRDSIFRSSC
610 620 630 640 650 660
EVVSKANCAK LKQGIAVRFH GEEGMGQGVV REWFDILSSE IVNPDYALFT QSADGTTFQP
670 680 690 700 710 720
NSNSSVNPDH LNYFRFAGQI LGLALNHRQL VNIYFTRSFY KHILGIPVNY QDVASIDPEY
730 740 750 760 770 780
AKNLQWILDN DISDLGLELT FSVETDVFGA MEEVPLKPGG ASILVTQENK AEYVQLVTEL
790 800 810 820 830 840
RMTRAIQPQI NAFLQGFHMF IPPSLIQLFD EYELELLLSG MPEIDVNDWL KNTEYTSGYE
850 860 870 880 890 900
RGDQVIQWFW DVVEELTQEE RVLLLQFVTG SSRVPHGGFA HIMGGSGLQN FTIAAVPYTA
910 920 930 940
NLLPTSSTCI NMLKLPEYPS KEILKDRLLV ALHCGSYGYT MA