Descriptions
Autoinhibitory domains (AIDs)
Target domain |
741-798 (SH3A domain) |
Relief mechanism |
|
Assay |
|
Target domain |
741-798 (SH3A domain) |
Relief mechanism |
PTM |
Assay |
|
Target domain |
1232-1418 (DH domain) |
Relief mechanism |
Partner binding, Ligand binding |
Assay |
|
Accessory elements
No accessory elements
References
- Gerth F et al. (2019) "Exon Inclusion Modulates Conformational Plasticity and Autoinhibition of the Intersectin 1 SH3A Domain", Structure (London, England : 1993), 27, 977-987.e5
- Ahmad KF et al. (2010) "The minimal autoinhibited unit of the guanine nucleotide exchange factor intersectin", PloS one, 5, e11291
- Murayama K et al. (2007) "Crystal structure of the rac activator, Asef, reveals its autoinhibitory mechanism", The Journal of biological chemistry, 282, 4238-4242
Autoinhibited structure
Activated structure
0 structures for E1BKY4
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|
184 variants for E1BKY4
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs439529730 | 2 | A>D | No | EVA | |
| rs476012578 | 10 | G>C | No | EVA | |
| rs477505865 | 32 | L>* | No | EVA | |
| rs450763072 | 50 | Q>H | No | EVA | |
| rs465400717 | 51 | S>P | No | EVA | |
| rs478601031 | 53 | L>* | No | EVA | |
| rs460133661 | 53 | L>F | No | EVA | |
| rs462567909 | 55 | Q>P | No | EVA | |
| rs452110544 | 61 | I>L | No | EVA | |
| rs450929961 | 69 | N>S | No | EVA | |
| rs477435175 | 70 | D>E | No | EVA | |
| rs466885686 | 135 | G>R | No | EVA | |
| rs481024594 | 141 | G>R | No | EVA | |
| rs468924005 | 143 | S>P | No | EVA | |
| rs480221575 | 154 | G>A | No | EVA | |
| rs443047359 | 155 | V>A | No | EVA | |
| rs438894815 | 159 | A>T | No | EVA | |
| rs473137989 | 166 | I>M | No | EVA | |
| rs136131917 | 166 | I>V | No | EVA | |
| rs436248835 | 167 | Q>P | No | EVA | |
| rs468987334 | 172 | F>I | No | EVA | |
| rs432079142 | 172 | F>S | No | EVA | |
| rs465066916 | 175 | P>L | No | EVA | |
| rs442973403 | 214 | E>Q | No | EVA | |
| rs450138993 | 289 | A>D | No | EVA | |
| rs433647070 | 351 | E>D | No | EVA | |
| rs455051880 | 352 | D>V | No | EVA | |
| rs481620380 | 357 | N>D | No | EVA | |
| rs451144258 | 361 | G>C | No | EVA | |
| rs477229652 | 370 | Q>P | No | EVA | |
| rs440497515 | 376 | Q>K | No | EVA | |
| rs460398751 | 382 | R>G | No | EVA | |
| rs441794698 | 382 | R>P | No | EVA | |
| rs462833460 | 386 | L>M | No | EVA | |
| rs444327169 | 391 | Q>P | No | EVA | |
| rs452118182 | 392 | E>G | No | EVA | |
| rs454573246 | 394 | K>E | No | EVA | |
| rs469572611 | 395 | E>G | No | EVA | |
| rs465235740 | 415 | K>N | No | EVA | |
| rs446794365 | 419 | L>P | No | EVA | |
| rs461415276 | 426 | E>D | No | EVA | |
| rs449341940 | 428 | R>S | No | EVA | |
| rs444390291 | 430 | E>A | No | EVA | |
| rs462737621 | 430 | E>Q | No | EVA | |
| rs458784623 | 434 | R>L | No | EVA | |
| rs456225890 | 435 | E>G | No | EVA | |
| rs459763810 | 456 | E>G | No | EVA | |
| rs466773816 | 572 | L>* | No | EVA | |
| rs448279027 | 573 | E>* | No | EVA | |
| rs462874915 | 573 | E>D | No | EVA | |
| rs460752940 | 593 | T>N | No | EVA | |
| rs444325009 | 605 | N>K | No | EVA | |
| rs458544485 | 606 | Q>K | No | EVA | |
| rs440141773 | 606 | Q>L | No | EVA | |
| rs1114991608 | 634 | E>V | No | EVA | |
| rs444307116 | 645 | Q>P | No | EVA | |
| rs470782165 | 646 | K>E | No | EVA | |
| rs466292031 | 662 | E>G | No | EVA | |
| rs715122641 | 685 | E>K | No | EVA | |
| rs523717040 | 715 | P>S | No | EVA | |
| rs450414825 | 737 | Q>K | No | EVA | |
| rs442775682 | 769 | V>G | No | EVA | |
| rs876380691 | 771 | E>G | No | EVA | |
| rs475929967 | 799 | I>T | No | EVA | |
| rs464346845 | 805 | P>H | No | EVA | |
| rs448008671 | 806 | A>T | No | EVA | |
| rs481028455 | 809 | K>N | No | EVA | |
| rs450488841 | 811 | V>E | No | EVA | |
| rs477452241 | 816 | S>P | No | EVA | |
| rs440522295 | 817 | T>P | No | EVA | |
| rs876591516 | 819 | T>P | No | EVA | |
| rs135047036 | 824 | V>G | No | EVA | |
| rs209431814 | 825 | C>G | No | EVA | |
| rs209431814 | 825 | C>R | No | EVA | |
| rs438920405 | 825 | C>S | No | EVA | |
| rs471845071 | 825 | C>W | No | EVA | |
| rs134032968 | 826 | E>G | No | EVA | |
| rs433735191 | 827 | T>P | No | EVA | |
| rs132732261 | 829 | A>P | No | EVA | |
| rs473313043 | 832 | S>P | No | EVA | |
| rs468802597 | 837 | E>A | No | EVA | |
| rs432060085 | 838 | P>T | No | EVA | |
| rs440819570 | 853 | P>T | No | EVA | |
| rs473791490 | 854 | T>P | No | EVA | |
| rs443345124 | 866 | D>N | No | EVA | |
| rs451939456 | 868 | W>C | No | EVA | |
| rs472963873 | 874 | L>I | No | EVA | |
| rs454326138 | 875 | T>P | No | EVA | |
| rs468547604 | 887 | A>D | No | EVA | |
| rs449947511 | 888 | F>L | No | EVA | |
| rs438067687 | 892 | T>P | No | EVA | |
| rs464461307 | 901 | V>G | No | EVA | |
| rs445024125 | 904 | Q>H | No | EVA | |
| rs474807979 | 912 | Q>R | No | EVA | |
| rs434657944 | 942 | D>E | No | EVA | |
| rs451181444 | 983 | A>E | No | EVA | |
| rs458962764 | 985 | L>Q | No | EVA | |
| rs440366181 | 986 | K>R | No | EVA | |
| rs461427460 | 987 | R>G | No | EVA | |
| rs441880956 | 989 | A>S | No | EVA | |
| rs444330932 | 990 | S>C | No | EVA | |
| rs136469912 | 990 | S>P | No | EVA | |
| rs519842173 | 1046 | P>A | No | EVA | |
| rs453038387 | 1061 | A>D | No | EVA | |
| rs434323513 | 1062 | G>R | No | EVA | |
| rs448910525 | 1072 | E>Q | No | EVA | |
| rs471141375 | 1074 | A>V | No | EVA | |
| rs435311991 | 1079 | S>F | No | EVA | |
| rs472447523 | 1079 | S>P | No | EVA | |
| rs716260909 | 1104 | G>S | No | EVA | |
| rs437662084 | 1112 | Q>K | No | EVA | |
| rs445552219 | 1112 | Q>R | No | EVA | |
| rs439448877 | 1123 | F>V | No | EVA | |
| rs479540708 | 1168 | E>A | No | EVA | |
| rs460914816 | 1169 | L>R | No | EVA | |
| rs476100988 | 1210 | T>P | No | EVA | |
| rs457571388 | 1215 | Q>R | No | EVA | |
| rs449060483 | 1246 | N>K | No | EVA | |
| rs444991572 | 1247 | Y>F | No | EVA | |
| rs463347743 | 1247 | Y>N | No | EVA | |
| rs459262559 | 1252 | Q>K | No | EVA | |
| rs440926033 | 1252 | Q>R | No | EVA | |
| rs452071445 | 1253 | L>P | No | EVA | |
| rs441953637 | 1294 | L>M | No | EVA | |
| rs474826141 | 1296 | V>D | No | EVA | |
| rs437085119 | 1297 | R>H | No | EVA | |
| rs475110633 | 1297 | R>S | No | EVA | |
| rs433018958 | 1300 | M>V | No | EVA | |
| rs465847320 | 1302 | G>A | No | EVA | |
| rs435146094 | 1306 | P>H | No | EVA | |
| rs468169541 | 1309 | M>T | No | EVA | |
| rs483331231 | 1311 | G>E | No | EVA | |
| rs458495667 | 1315 | T>A | No | EVA | |
| rs458495667 | 1315 | T>P | No | EVA | |
| rs479294251 | 1325 | I>T | No | EVA | |
| rs456445472 | 1326 | R>L | No | EVA | |
| rs442096963 | 1327 | F>L | No | EVA | |
| rs481173520 | 1330 | C>F | No | EVA | |
| rs444467441 | 1337 | L>W | No | EVA | |
| rs453776144 | 1343 | D>E | No | EVA | |
| rs439350875 | 1343 | D>G | No | EVA | |
| rs451613685 | 1343 | D>H | No | EVA | |
| rs451613685 | 1343 | D>N | No | EVA | |
| rs435287184 | 1344 | E>* | No | EVA | |
| rs456062825 | 1344 | E>A | No | EVA | |
| rs446341617 | 1349 | K>M | No | EVA | |
| rs478985205 | 1350 | E>D | No | EVA | |
| rs462936636 | 1352 | V>A | No | EVA | |
| rs448454173 | 1352 | V>L | No | EVA | |
| rs438436930 | 1415 | V>M | No | EVA | |
| rs454945431 | 1416 | N>D | No | EVA | |
| rs450951032 | 1428 | L>P | No | EVA | |
| rs464500505 | 1437 | C>G | No | EVA | |
| rs381467830 | 1470 | N>S | No | EVA | |
| rs471456784 | 1522 | V>G | No | EVA | |
| rs210301142 | 1574 | K>M | No | EVA | |
| rs455985123 | 1612 | S>G | No | EVA | |
| rs470349598 | 1613 | N>K | No | EVA | |
| rs451836829 | 1615 | Y>D | No | EVA | |
| rs211596095 | 1616 | C>W | No | EVA | |
| rs447549622 | 1617 | E>G | No | EVA | |
| rs467656912 | 1627 | T>P | No | EVA | |
| rs463512101 | 1665 | S>P | No | EVA | |
| rs465561425 | 1669 | F>S | No | EVA | |
| rs467864019 | 1675 | I>V | No | EVA | |
| rs482261509 | 1678 | A>G | No | EVA | |
| rs463612016 | 1684 | Q>P | No | EVA | |
| rs477147557 | 1691 | T>S | No | EVA | |
| rs458532858 | 1693 | C>G | No | EVA | |
| rs473108842 | 1694 | L>P | No | EVA | |
| rs454560523 | 1696 | L>P | No | EVA | |
| rs475377606 | 1697 | H>L | No | EVA | |
| rs432634066 | 1698 | E>V | No | EVA | |
| rs465667546 | 1700 | P>R | No | EVA | |
| rs482416019 | 1701 | T>R | No | EVA | |
| rs468026786 | 1701 | T>S | No | EVA | |
| rs445206457 | 1704 | I>F | No | EVA | |
| rs477063987 | 1704 | I>M | No | EVA | |
| rs440156759 | 1705 | V>G | No | EVA | |
| rs442491239 | 1706 | V>F | No | EVA | |
| rs438438519 | 1707 | R>H | No | EVA | |
| rs475541073 | 1707 | R>S | No | EVA | |
| rs435101877 | 1715 | E>D | No | EVA | |
| rs455953619 | 1717 | P>E | No | EVA |
No associated diseases with E1BKY4
16 regional properties for E1BKY4
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | C2 domain | 1574 - 1690 | IPR000008 |
| domain | Dbl homology (DH) domain | 1232 - 1418 | IPR000219 |
| domain | EH domain | 14 - 108 | IPR000261-1 |
| domain | EH domain | 214 - 310 | IPR000261-2 |
| conserved_site | Guanine-nucleotide dissociation stimulator, CDC24, conserved site | 1366 - 1391 | IPR001331 |
| domain | SH3 domain | 740 - 801 | IPR001452-1 |
| domain | SH3 domain | 908 - 966 | IPR001452-2 |
| domain | SH3 domain | 997 - 1055 | IPR001452-3 |
| domain | SH3 domain | 1069 - 1133 | IPR001452-4 |
| domain | SH3 domain | 1150 - 1209 | IPR001452-5 |
| domain | Pleckstrin homology domain | 1435 - 1588 | IPR001849 |
| domain | EF-hand domain | 53 - 88 | IPR002048-1 |
| domain | EF-hand domain | 254 - 289 | IPR002048-2 |
| binding_site | EF-Hand 1, calcium-binding site | 66 - 78 | IPR018247-1 |
| binding_site | EF-Hand 1, calcium-binding site | 267 - 279 | IPR018247-2 |
| domain | Intersectin-1, AP2 binding region | 798 - 912 | IPR032140 |
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| cell projection | A prolongation or process extending from a cell, e.g. a flagellum or axon. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| intracellular vesicle | Any vesicle that is part of the intracellular region. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| presynaptic membrane | A specialized area of membrane of the axon terminal that faces the plasma membrane of the neuron or muscle fiber with which the axon terminal establishes a synaptic junction; many synaptic junctions exhibit structural presynaptic characteristics, such as conical, electron-dense internal protrusions, that distinguish it from the remainder of the axon plasma membrane. |
3 GO annotations of molecular function
| Name | Definition |
|---|---|
| calcium ion binding | Binding to a calcium ion (Ca2+). |
| guanyl-nucleotide exchange factor activity | Stimulates the exchange of GDP to GTP on a signaling GTPase, changing its conformation to its active form. Guanine nucleotide exchange factors (GEFs) act by stimulating the release of guanosine diphosphate (GDP) to allow binding of guanosine triphosphate (GTP), which is more abundant in the cell under normal cellular physiological conditions. |
| molecular adaptor activity | The binding activity of a molecule that brings together two or more molecules through a selective, non-covalent, often stoichiometric interaction, permitting those molecules to function in a coordinated way. |
4 GO annotations of biological process
| Name | Definition |
|---|---|
| clathrin-dependent synaptic vesicle endocytosis | Clathrin-dependent endocytosis of presynaptic membrane regions comprising synaptic vesicles' membrane constituents. This is a relatively slow process occurring in the range of tens of seconds. |
| endosomal transport | The directed movement of substances mediated by an endosome, a membrane-bounded organelle that carries materials enclosed in the lumen or located in the endosomal membrane. |
| intracellular signal transduction | The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell. |
| lipid catabolic process | The chemical reactions and pathways resulting in the breakdown of lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent. |
7 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| A0A2I2U1K8 | ITSN1 | Intersectin 1 | Felis catus (Cat) (Felis silvestris catus) | SS |
| A0A2I3T257 | ITSN1 | Intersectin 1 | Pan troglodytes (Chimpanzee) | SS |
| Q9NZM3 | ITSN2 | Intersectin-2 | Homo sapiens (Human) | SS |
| Q15811 | ITSN1 | Intersectin-1 | Homo sapiens (Human) | EV SS |
| Q9Z0R6 | Itsn2 | Intersectin-2 | Mus musculus (Mouse) | SS |
| Q9Z0R4 | Itsn1 | Intersectin-1 | Mus musculus (Mouse) | EV |
| Q9WVE9 | Itsn1 | Intersectin-1 | Rattus norvegicus (Rat) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MAQFPTPFGG | SLDIWAITVE | ERAKHDQQFH | SLKPISGFIT | GDQARNFFFQ | SGLPQPVLAQ |
| 70 | 80 | 90 | 100 | 110 | 120 |
| IWALADMNND | GRMDQVEFSI | AMKLIKLKLQ | GYQLPSALPP | VMKQQPVAIS | SAPAFGMGGI |
| 130 | 140 | 150 | 160 | 170 | 180 |
| ASMPPLTAVA | PVPMGSIPVV | GMSPPLVSSV | PAAGVPPLAN | GAPPVIQPLP | AFAHPAATLP |
| 190 | 200 | 210 | 220 | 230 | 240 |
| KSSSFSRSGP | GSQLNTKLQK | AQSFDVASVP | PTAEWAVPQS | SRLKYRQLFN | SHDKTMSGHL |
| 250 | 260 | 270 | 280 | 290 | 300 |
| TGPQARTILM | QSSLPQAQLA | SIWNLSDIDQ | DGKLTAEEFI | LAMHLIDVAM | SGQPLPPVLP |
| 310 | 320 | 330 | 340 | 350 | 360 |
| PEYIPPSFRR | VRSGSGISVI | TSTSVDQRLP | EEPVLEDEQQ | QLEKKLPVTF | EDKKRENFER |
| 370 | 380 | 390 | 400 | 410 | 420 |
| GNLELEKRRQ | ALLEQQRKEQ | ERLAQLERAE | QERKERERQE | QERKRQLELE | KQLEKQRELE |
| 430 | 440 | 450 | 460 | 470 | 480 |
| RQREEERRKE | IERREAAKRE | LERQRQLEWE | RNRRQELLNQ | RNKEQEDIVV | LKAKKKTLEF |
| 490 | 500 | 510 | 520 | 530 | 540 |
| ELEALNDKKH | QLEGKLQDIR | CRLTTQRQEI | ESTNKSRELR | IAEITHLQQQ | LQESQQMLGR |
| 550 | 560 | 570 | 580 | 590 | 600 |
| LIPEKQILND | QLKQVQQNSL | HRDSLLTLKR | ALEAKELARQ | QLRDQLDEVE | KETRSKLQEI |
| 610 | 620 | 630 | 640 | 650 | 660 |
| DIFNNQLKEL | REIHNKQQLQ | KQKSIEAERL | KQKEQERKIL | ELEKQKEEAQ | RRAQERDQQW |
| 670 | 680 | 690 | 700 | 710 | 720 |
| LERVHQEDEQ | QRPRKPHEEE | KLKREESVKK | KDSEEKGKPE | MQDKLSRLFH | QHQEPAKPAV |
| 730 | 740 | 750 | 760 | 770 | 780 |
| QAPWSTAEKG | PLTISAQENI | KVVYYRALYP | FESRSHDEIT | IQPGDIVMVD | ESQTGEPGWL |
| 790 | 800 | 810 | 820 | 830 | 840 |
| GGELKGKTGW | FPANYAEKIP | ENEVPAPVKP | VTEAASTPTP | KVAVCETPAP | SSTTPAEPSE |
| 850 | 860 | 870 | 880 | 890 | 900 |
| TPNNWADFSS | TWPTSTNEKP | ETDSWDAWAT | QPSLTVPSAG | QLRQRSAFTP | ATATGSSPSP |
| 910 | 920 | 930 | 940 | 950 | 960 |
| VLGQGEKVEG | LQAQALYPWR | AKKDNHLNFN | KNDVITVLEQ | QDMWWFGEVQ | GQKGWFPKSY |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| VKLISGPIRK | STSMDSGPSE | SPASLKRVAS | PAAKPAVSGE | EFIAMYTYES | SEQGDLTFQQ |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| GDVILVTKKD | GDWWTGTVGD | KSGVFPSNYV | RLKDSEGSGT | AGKTGSLGKK | PEIAQVIASY |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| TATGPEQLTL | APGQLILIRK | KNPGGWWEGE | LQARGKKRQI | GWFPANYVKL | LSPGTSKITP |
| 1150 | 1160 | 1170 | 1180 | 1190 | 1200 |
| TDPPKPTAFP | TVCQVIGMYD | YTAQNDDELA | FSKGQVINVL | NKEDPDWWKG | EVHGQVGLFP |
| 1210 | 1220 | 1230 | 1240 | 1250 | 1260 |
| SNYVKLTTDT | DPSQQWCSDL | HLLDMLTPTE | RKRQGYIHEL | IVTEENYVND | LQLVTEIFQK |
| 1270 | 1280 | 1290 | 1300 | 1310 | 1320 |
| PLMESELLTE | KEGAMIFVNW | KELIMCNIKL | LKALRVRKKM | SGEKMPVKMI | GDILTAQLPH |
| 1330 | 1340 | 1350 | 1360 | 1370 | 1380 |
| MQPYIRFCSC | QLNGAALIQQ | KTDEAPDFKE | FVKRLAMDPR | CKGMPLSSFI | LKPMQRVTRY |
| 1390 | 1400 | 1410 | 1420 | 1430 | 1440 |
| PLIIKNILEN | TPENHPDHSH | LKHALEKAEE | LCSQVNEGVR | EKENSDRLEW | IQAHVLCEGL |
| 1450 | 1460 | 1470 | 1480 | 1490 | 1500 |
| SEQLVFNSVT | NCLGPRKFLH | SGKLYKAKSN | KELYGFLFND | FLLLTQIIKP | LGSSGTDKVF |
| 1510 | 1520 | 1530 | 1540 | 1550 | 1560 |
| SPKSNLQYKM | YKTPIFLNEV | LVKLPTDPSG | DEPIFHISHI | DRVYTLRAES | INERTAWVQK |
| 1570 | 1580 | 1590 | 1600 | 1610 | 1620 |
| IKAASELYIE | TEKKKREKAY | LVRSQRATGI | GRLMVNVVEG | IELKPCRSHG | KSNPYCEVTM |
| 1630 | 1640 | 1650 | 1660 | 1670 | 1680 |
| GSQCHITKTI | QDTLNPKWNS | NCQFFIRDLE | QEVLCITVFE | RDQFSPDDFL | GRTEIRVADI |
| 1690 | 1700 | 1710 | |||
| KKDQGSKGPV | TKCLLLHEVP | TGEIVVRLDL | QLFDEP |