D3K0R6
Gene name |
ATP2B4 |
Protein name |
Plasma membrane calcium-transporting ATPase 4 |
Names |
PMCA4 , EC 7.2.2.10 |
Species |
Bos taurus (Bovine) |
KEGG Pathway |
bta:282146 |
EC number |
7.2.2.10: Linked to the hydrolysis of a nucleoside triphosphate |
Protein Class |
|
Descriptions
Autoinhibitory domains (AIDs)
Target domain |
346-1051 (P-type ATPase domain) |
Relief mechanism |
Partner binding, Ligand binding |
Assay |
|
Target domain |
445-1046 (P-type ATPase domain) |
Relief mechanism |
Partner binding |
Assay |
|
Target domain |
445-1046 (P-type ATPase domain) |
Relief mechanism |
Partner binding, Ligand binding |
Assay |
|
Accessory elements
No accessory elements
References
- Calì T et al. (2017) "The ataxia related G1107D mutation of the plasma membrane Ca(2+) ATPase isoform 3 affects its interplay with calmodulin and the autoinhibition process", Biochimica et biophysica acta. Molecular basis of disease, 1863, 165-173
- Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell systems, 2, 89-100
- Carafoli E (1994) "Biogenesis: plasma membrane calcium ATPase: 15 years of work on the purified enzyme", FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 8, 993-1002
- Tidow H et al. (2010) "Expression, purification, crystallization and preliminary X-ray analysis of calmodulin in complex with the regulatory domain of the plasma-membrane Ca2+-ATPase ACA8", Acta crystallographica. Section F, Structural biology and crystallization communications, 66, 361-3
- Saffioti NA et al. (2021) "Conformational changes during the reaction cycle of plasma membrane Ca2+-ATPase in the autoinhibited and activated states", The Biochemical journal, 478, 2019-2034
- Calì T et al. (2018) "The PMCA pumps in genetically determined neuronal pathologies", Neuroscience letters, 663, 2-11
- Corradi GR et al. (2007) "Intramolecular fluorescence resonance energy transfer between fused autofluorescent proteins reveals rearrangements of the N- and C-terminal segments of the plasma membrane Ca2+ pump involved in the activation", The Journal of biological chemistry, 282, 35440-8
- Osborn KD et al. (2005) "Single-molecule characterization of the dynamics of calmodulin bound to oxidatively modified plasma-membrane Ca2+-ATPase", Biochemistry, 44, 11074-81
- Lopreiato R et al. (2014) "The plasma membrane calcium pump: new ways to look at an old enzyme", The Journal of biological chemistry, 289, 10261-10268
- Bredeston LM et al. (2004) "Loss of autoinhibition of the plasma membrane Ca(2+) pump by substitution of aspartic 170 by asparagin. A ctivation of plasma membrane calcium ATPase 4 without disruption of the interaction between the catalytic core and the C-terminal regulatory domain", The Journal of biological chemistry, 279, 41619-25
- Pinto Fde T et al. (2002) "Deletions in the acidic lipid-binding region of the plasma membrane Ca2+ pump. A mutant with high affinity for Ca2+ resembling the acidic lipid-activated enzyme", The Journal of biological chemistry, 277, 12784-9
Autoinhibited structure
Activated structure
1 structures for D3K0R6
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-D3K0R6-F1 | Predicted | AlphaFoldDB |
No variants for D3K0R6
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for D3K0R6 | |||||
No associated diseases with D3K0R6
5 regional properties for D3K0R6
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Cation-transporting P-type ATPase, N-terminal | 45 - 121 | IPR004014 |
| domain | Cation-transporting P-type ATPase, C-terminal | 868 - 1046 | IPR006068 |
| ptm | P-type ATPase, phosphorylation site | 465 - 471 | IPR018303 |
| domain | Plasma membrane calcium transporting P-type ATPase, C-terminal | 1089 - 1135 | IPR022141 |
| domain | P-type ATPase, haloacid dehalogenase domain | 445 - 835 | IPR044492 |
Functions
| Description | ||
|---|---|---|
| EC Number | 7.2.2.10 | Linked to the hydrolysis of a nucleoside triphosphate |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| intracellular membrane-bounded organelle | Organized structure of distinctive morphology and function, bounded by a single or double lipid bilayer membrane and occurring within the cell. Includes the nucleus, mitochondria, plastids, vacuoles, and vesicles. Excludes the plasma membrane. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| sperm flagellum | A microtubule-based flagellum (or cilium) that is part of a sperm, a mature male germ cell that develops from a spermatid. |
6 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ATP hydrolysis activity | Catalysis of the reaction |
| calmodulin binding | Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states. |
| metal ion binding | Binding to a metal ion. |
| P-type calcium transporter activity | Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction |
| PDZ domain binding | Binding to a PDZ domain of a protein, a domain found in diverse signaling proteins. |
5 GO annotations of biological process
| Name | Definition |
|---|---|
| cellular response to acetylcholine | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an acetylcholine stimulus. |
| flagellated sperm motility | The directed, self-propelled movement of a cilium (aka flagellum) that contributes to the movement of a flagellated sperm. |
| intracellular calcium ion homeostasis | A homeostatic process involved in the maintenance of a steady state level of calcium ions within a cell. |
| regulation of cytosolic calcium ion concentration | Any process involved in the maintenance of an internal steady state of calcium ions within the cytosol of a cell or between the cytosol and its surroundings. |
| urinary bladder smooth muscle contraction | A process in which force is generated within smooth muscle tissue, resulting in a change in muscle geometry. This process occurs in the urinary bladder. Force generation involves a chemo-mechanical energy conversion step that is carried out by the actin/myosin complex activity, which generates force through ATP hydrolysis. The urinary bladder is a musculomembranous sac along the urinary tract. |
31 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P38929 | PMC1 | Calcium-transporting ATPase 2 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q16720 | ATP2B3 | Plasma membrane calcium-transporting ATPase 3 | Homo sapiens (Human) | EV |
| Q01814 | ATP2B2 | Plasma membrane calcium-transporting ATPase 2 | Homo sapiens (Human) | SS |
| P20020 | ATP2B1 | Plasma membrane calcium-transporting ATPase 1 | Homo sapiens (Human) | SS |
| P23634 | ATP2B4 | Plasma membrane calcium-transporting ATPase 4 | Homo sapiens (Human) | EV |
| G5E829 | Atp2b1 | Plasma membrane calcium-transporting ATPase 1 | Mus musculus (Mouse) | SS |
| Q9R0K7 | Atp2b2 | Plasma membrane calcium-transporting ATPase 2 | Mus musculus (Mouse) | SS |
| Q6Q477 | Atp2b4 | Plasma membrane calcium-transporting ATPase 4 | Mus musculus (Mouse) | SS |
| P23220 | ATP2B1 | Plasma membrane calcium-transporting ATPase 1 | Sus scrofa (Pig) | SS |
| P11505 | Atp2b1 | Plasma membrane calcium-transporting ATPase 1 | Rattus norvegicus (Rat) | SS |
| Q64568 | Atp2b3 | Plasma membrane calcium-transporting ATPase 3 | Rattus norvegicus (Rat) | SS |
| P11506 | Atp2b2 | Plasma membrane calcium-transporting ATPase 2 | Rattus norvegicus (Rat) | SS |
| Q64542 | Atp2b4 | Plasma membrane calcium-transporting ATPase 4 | Rattus norvegicus (Rat) | SS |
| Q2QMX9 | ACA10 | Calcium-transporting ATPase 10, plasma membrane-type | Oryza sativa subsp japonica (Rice) | SS |
| Q2QY12 | ACA9 | Probable calcium-transporting ATPase 9, plasma membrane-type | Oryza sativa subsp japonica (Rice) | SS |
| Q6ATV4 | ACA3 | Calcium-transporting ATPase 3, plasma membrane-type | Oryza sativa subsp japonica (Rice) | SS |
| Q7X8B5 | ACA5 | Calcium-transporting ATPase 5, plasma membrane-type | Oryza sativa subsp japonica (Rice) | SS |
| Q7XEK4 | ACA7 | Calcium-transporting ATPase 7, plasma membrane-type | Oryza sativa subsp japonica (Rice) | EV |
| Q8RUN1 | ACA1 | Calcium-transporting ATPase 1, plasma membrane-type | Oryza sativa subsp japonica (Rice) | SS |
| Q65X71 | ACA6 | Probable calcium-transporting ATPase 6, plasma membrane-type | Oryza sativa subsp japonica (Rice) | SS |
| Q2RAS0 | ACA8 | Probable calcium-transporting ATPase 8, plasma membrane-type | Oryza sativa subsp japonica (Rice) | SS |
| O22218 | ACA4 | Calcium-transporting ATPase 4, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| O64806 | ACA7 | Putative calcium-transporting ATPase 7, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| O81108 | ACA2 | Calcium-transporting ATPase 2, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | EV |
| Q9LF79 | ACA8 | Calcium-transporting ATPase 8, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | EV |
| Q9LIK7 | ACA13 | Putative calcium-transporting ATPase 13, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9LU41 | ACA9 | Calcium-transporting ATPase 9, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9LY77 | ACA12 | Calcium-transporting ATPase 12, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9M2L4 | ACA11 | Putative calcium-transporting ATPase 11, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9SZR1 | ACA10 | Calcium-transporting ATPase 10, plasma membrane-type | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q37145 | ACA1 | Calcium-transporting ATPase 1 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MTNPTEHTLP | SNSILESREG | EFGCTVMDLR | KLMELRSSDA | IDQINVHYGG | VMNLCSRLKT |
| 70 | 80 | 90 | 100 | 110 | 120 |
| NPVEGLSGNP | ADLEKRKQVF | GQNLIPPKKP | KTFLELVWEA | LQDVTLIILE | IAAIISLVLS |
| 130 | 140 | 150 | 160 | 170 | 180 |
| FYRPPGGENE | QCGLAVTSPE | DEGEAEAGWI | EGAAILFSVI | IVVLVTAFND | WSKEKQFRGL |
| 190 | 200 | 210 | 220 | 230 | 240 |
| QNRIEKEQKF | SVIRNGHIIQ | LPVAEIVVGD | IAQIKYGDLL | PADGILIQGN | DLKIDESSLT |
| 250 | 260 | 270 | 280 | 290 | 300 |
| GESDHVKKSL | ERDPMLLSGT | HVMEGSGRMV | VTAVGINSQT | GIIFTLLGAS | EGEEEEKKKK |
| 310 | 320 | 330 | 340 | 350 | 360 |
| GKKQGVPENR | NKAKTQDGVA | LEIQPLNSQE | GIDSEEKEKK | AAKLPKKEKS | VLQGKLTRLA |
| 370 | 380 | 390 | 400 | 410 | 420 |
| VQIGKAGLIM | SAITVLILIL | YFVIDNFVIQ | RRPWLAECTP | IYVQYFVKFF | IIGVTVLVVA |
| 430 | 440 | 450 | 460 | 470 | 480 |
| VPEGLPLAVT | ISLAYSVKKM | MKDNNLVRHL | DACETMGNAT | AICSDKTGTL | TMNRMSVVQA |
| 490 | 500 | 510 | 520 | 530 | 540 |
| YIGDTRYHQI | PSPDDLVPKV | LDLIVNGISI | NSAYTSKILP | PEKEGGLPRQ | VGNKTECALL |
| 550 | 560 | 570 | 580 | 590 | 600 |
| GFVSDLKQDY | HAVRSEVPEE | KLYKVYTFNS | VRKSMSTVIE | KPGGGYRMYS | KGASEIILRK |
| 610 | 620 | 630 | 640 | 650 | 660 |
| CNRILDKKGE | AVPFKNKDRD | EMVRTVIEPM | ACEGLRTLCI | AYRDFNDGEP | PWDNESEILT |
| 670 | 680 | 690 | 700 | 710 | 720 |
| ELTCIAVVGI | EDPVRPEVPE | AIAKCKRAGI | TVRMVTGDNI | NTARAIATKC | GIVTPGDDFL |
| 730 | 740 | 750 | 760 | 770 | 780 |
| CLEGKEFNRL | IRNEKGEVEQ | EKLDKIWPKL | RVLARSSPTD | KHTLVKGIID | STVGDQRQVV |
| 790 | 800 | 810 | 820 | 830 | 840 |
| AVTGDGTNDG | PALKKADVGF | AMGIAGTDVA | KEASDIILTD | DNFTSIVKAV | MWGRNVYDSI |
| 850 | 860 | 870 | 880 | 890 | 900 |
| SKFLQFQLTV | NVVAVIVAFT | GACITQDSPL | KAVQMLWVNL | IMDTFASLAL | ATEPPTDSLL |
| 910 | 920 | 930 | 940 | 950 | 960 |
| KRRPYGRNKP | LISRTMMKNI | LGHAVYQLTV | IFFLVFAGEK | FFDIDSGRRA | PLHSPPSQHY |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| TIIFNTFVLM | QLFNEINSRK | IHGERNVFSG | IFRNLIFCSV | VLGTFISQII | IVEFGGKPFS |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| CTKLTLSQWF | WCLFIGIGEL | LWGQVISTIP | TQSLKFLKEA | GHGTTKEEIT | KDAEGLDEID |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| HAEMELRRGQ | ILWFRGLNRI | QTQIKVVKAF | HSSLHESIQK | PKNQNSIHNF | MTHPEFTIDE |
| 1150 | 1160 | 1170 | 1180 | 1190 | 1200 |
| EGPRTPLLDE | QEEEIFEKVS | KPGTKTSSLD | GEVTPQTNKN | NNTVDCCQVQ | IVASHSDSPL |
| HSLETSV |