D0ZVG2

EV
Gene name
sspH1 (STM14_1483)
Protein name
E3 ubiquitin-protein ligase SspH1
Names
RING-type E3 ubiquitin transferase SspH1, Salmonella secreted protein H1, Secreted effector protein SspH1
Species
Salmonella typhimurium (strain 14028s / SGSC 2262)
KEGG Pathway
seo:STM14_1483
EC number
2.3.2.27: Aminoacyltransferases
Protein Class
FAMILY NOT NAMED (PTHR47114)

Descriptions

SspH1 is a Salmonella enterica serovar Typhimurium TTSS effector that localizes to the mammalian nucleus and catalyzes the ubiquitination and proteasome-dependent degradation of PKN1 in cells, which attenuates androgen receptor responsiveness. In the absence of substrate, the leucine-rich repeat (LRR) domain inhibits the catalytic domain (NEL domain). Binding of HR1b domain of PKN1 to the LRR domain relieves the autoinhibition.

Autoinhibitory domains (AIDs)

162-397 (LRR domain) EV

Target domain

404-700 (E3 ubiquitin-protein ligase catalytic domain.)

Relief mechanism

Partner binding

Assay

Deletion assay, Structural analysis

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

3 structures for D0ZVG2

Entry ID Method Resolution Chain Position Source
4NKG X-ray 290 A A/C 161-405 PDB
4NKH X-ray 275 A A/B/C/D/E/F 161-398 PDB
AF-D0ZVG2-F1 Predicted AlphaFoldDB

No variants for D0ZVG2

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for D0ZVG2

No associated diseases with D0ZVG2

3 regional properties for D0ZVG2

Type Name Position InterPro Accession
repeat Leucine-rich repeat 298 - 319 IPR001611-1
repeat Leucine-rich repeat 338 - 360 IPR001611-2
domain Novel E3 ligase domain 414 - 626 IPR029487

Functions

Description
EC Number 2.3.2.27 Aminoacyltransferases
Subcellular Localization
  • Secreted
  • Host cytoplasm
  • Host nucleus
  • Secreted via type III secretion systems 1 and 2 (SPI-1 and SPI-2 T3SS), and delivered into the host cytoplasm
  • Localizes predominantly to the host nucleus
PANTHER Family PTHR47114 FAMILY NOT NAMED
PANTHER Subfamily PTHR47114:SF2 OLIGODENDROCYTE-MYELIN GLYCOPROTEIN
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
extracellular region The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
host cell cytoplasm The cytoplasm of a host cell.
host cell nucleus A membrane-bounded organelle as it is found in the host cell in which chromosomes are housed and replicated. The host is defined as the larger of the organisms involved in a symbiotic interaction.

1 GO annotations of molecular function

Name Definition
ubiquitin-protein transferase activity Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages.

2 GO annotations of biological process

Name Definition
protein secretion by the type III secretion system The process in which proteins are transferred into the extracellular milieu or directly into host cells by the bacterial type III secretion system; secretion occurs in a continuous process without the distinct presence of periplasmic intermediates and does not involve proteolytic processing of secreted proteins.
protein ubiquitination The process in which one or more ubiquitin groups are added to a protein.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MFNIRNTQPS VSMQAIAGAA APEASPEEIV WEKIQVFFPQ ENYEEAQQCL AELCHPARGM
70 80 90 100 110 120
LPDHISSQFA RLKALTFPAW EENIQCNRDG INQFCILDAG SKEILSITLD DAGNYTVNCQ
130 140 150 160 170 180
GYSEAHDFIM DTEPGEECTE FAEGASGTSL RPATTVSQKA AEYDAVWSKW ERDAPAGESP
190 200 210 220 230 240
GRAAVVQEMR DCLNNGNPVL NVGASGLTTL PDRLPPHITT LVIPDNNLTS LPELPEGLRE
250 260 270 280 290 300
LEVSGNLQLT SLPSLPQGLQ KLWAYNNWLA SLPTLPPGLG DLAVSNNQLT SLPEMPPALR
310 320 330 340 350 360
ELRVSGNNLT SLPALPSGLQ KLWAYNNRLT SLPEMSPGLQ ELDVSHNQLT RLPQSLTGLS
370 380 390 400 410 420
SAARVYLDGN PLSVRTLQAL RDIIGHSGIR IHFDMAGPSV PREARALHLA VADWLTSARE
430 440 450 460 470 480
GEAAQADRWQ AFGLEDNAAA FSLVLDRLRE TENFKKDAGF KAQISSWLTQ LAEDAALRAK
490 500 510 520 530 540
TFAMATEATS TCEDRVTHAL HQMNNVQLVH NAEKGEYDNN LQGLVSTGRE MFRLATLEQI
550 560 570 580 590 600
AREKAGTLAL VDDVEVYLAF QNKLKESLEL TSVTSEMRFF DVSGVTVSDL QAAELQVKTA
610 620 630 640 650 660
ENSGFSKWIL QWGPLHSVLE RKVPERFNAL REKQISDYED TYRKLYDEVL KSSGLVDDTD
670 680 690
AERTIGVSAM DSAKKEFLDG LRALVDEVLG SYLTARWRLN