D0ZRB2
Gene name |
slrP (STM14_928) |
Protein name |
E3 ubiquitin-protein ligase SlrP |
Names |
EC 2.3.2.27 , RING-type E3 ubiquitin transferase SlrP , Secreted effector protein SlrP |
Species |
Salmonella typhimurium (strain 14028s / SGSC 2262) |
KEGG Pathway |
seo:STM14_928 |
EC number |
2.3.2.27: Aminoacyltransferases |
Protein Class |
FAMILY NOT NAMED (PTHR47114) |
Descriptions
Autoinhibition of SlrP, a type III secretion system effector from the NEL family, involves structural constraints that sequester the NEL domain. These constraints are proposed to be sequentially released upon interaction with host substrates, leading to E3 ubiquitin ligase activity modulation.
Autoinhibitory domains (AIDs)
Target domain |
465-765 (NEL domain) |
Relief mechanism |
PTM |
Assay |
Structural analysis |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
2 structures for D0ZRB2
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 4PUF | X-ray | 330 A | A/B | 141-765 | PDB |
| AF-D0ZRB2-F1 | Predicted | AlphaFoldDB |
No variants for D0ZRB2
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for D0ZRB2 | |||||
No associated diseases with D0ZRB2
8 regional properties for D0ZRB2
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| repeat | Leucine-rich repeat | 263 - 284 | IPR001611 |
| repeat | Leucine-rich repeat, typical subtype | 199 - 220 | IPR003591-1 |
| repeat | Leucine-rich repeat, typical subtype | 260 - 284 | IPR003591-2 |
| repeat | Leucine-rich repeat, typical subtype | 302 - 326 | IPR003591-3 |
| repeat | Leucine-rich repeat, typical subtype | 346 - 368 | IPR003591-4 |
| repeat | Leucine-rich repeat, typical subtype | 370 - 389 | IPR003591-5 |
| domain | Novel E3 ligase domain | 472 - 680 | IPR029487 |
| domain | LRR-containing bacterial E3 ligase, N-terminal | 107 - 170 | IPR032674 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.3.2.27 | Aminoacyltransferases |
| Subcellular Localization |
|
|
| PANTHER Family | PTHR47114 | FAMILY NOT NAMED |
| PANTHER Subfamily | PTHR47114:SF2 | OLIGODENDROCYTE-MYELIN GLYCOPROTEIN |
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
2 GO annotations of cellular component
| Name | Definition |
|---|---|
| extracellular region | The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite. |
| host cell cytoplasm | The cytoplasm of a host cell. |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| ubiquitin protein ligase activity | Catalysis of the transfer of ubiquitin to a substrate protein via the reaction X-ubiquitin + S -> X + S-ubiquitin, where X is either an E2 or E3 enzyme, the X-ubiquitin linkage is a thioester bond, and the S-ubiquitin linkage is an amide bond |
| ubiquitin-protein transferase activity | Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages. |
3 GO annotations of biological process
| Name | Definition |
|---|---|
| killing of cells of another organism | Any process in an organism that results in the killing of cells of another organism, including in some cases the death of the other organism. Killing here refers to the induction of death in one cell by another cell, not cell-autonomous death due to internal or other environmental conditions. |
| protein secretion by the type III secretion system | The process in which proteins are transferred into the extracellular milieu or directly into host cells by the bacterial type III secretion system; secretion occurs in a continuous process without the distinct presence of periplasmic intermediates and does not involve proteolytic processing of secreted proteins. |
| protein ubiquitination | The process in which one or more ubiquitin groups are added to a protein. |
No homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| No homologous proteins | ||||
| 10 | 20 | 30 | 40 | 50 | 60 |
| MFNITNIQST | ARHQSISNEA | STEVPLKEEI | WNKISAFFSS | EHQVEAQNCI | AYLCHPPETA |
| 70 | 80 | 90 | 100 | 110 | 120 |
| SPEEIKSKFE | CLRMLAFPAY | ADNIQYSRGG | ADQYCILSEN | SQEILSIVFN | TEGYTVEGGG |
| 130 | 140 | 150 | 160 | 170 | 180 |
| KSVTYTRVTE | SEQASSASGS | KDAVNYELIW | SEWVKEAPAK | EAANREEAVQ | RMRDCLKNNK |
| 190 | 200 | 210 | 220 | 230 | 240 |
| TELRLKILGL | TTIPAYIPEQ | ITTLILDNNE | LKSLPENLQG | NIKTLYANSN | QLTSIPATLP |
| 250 | 260 | 270 | 280 | 290 | 300 |
| DTIQEMELSI | NRITELPERL | PSALQSLDLF | HNKISCLPEN | LPEELRYLSV | YDNSIRTLPA |
| 310 | 320 | 330 | 340 | 350 | 360 |
| HLPSEITHLN | VQSNSLTALP | ETLPPGLKTL | EAGENALTSL | PASLPPELQV | LDVSKNQITV |
| 370 | 380 | 390 | 400 | 410 | 420 |
| LPETLPPTIT | TLDVSRNALT | NLPENLPAAL | QIMQASRNNL | VRLPESLPHF | RGEGPQPTRI |
| 430 | 440 | 450 | 460 | 470 | 480 |
| IVEYNPFSER | TIQNMQRLMS | SVDYQGPRVL | FAMGDFSIVR | VTRPLHQAVQ | GWLTSLEEED |
| 490 | 500 | 510 | 520 | 530 | 540 |
| VNQWRAFEAE | ANAAAFSGFL | DYLGDTQNTR | HPDFKEQVSA | WLMRLAEDSA | LRETVFIIAM |
| 550 | 560 | 570 | 580 | 590 | 600 |
| NATISCEDRV | TLAYHQMQEA | TLVHDAERGA | FDSHLAELIM | AGREIFRLEQ | IESLAREKVK |
| 610 | 620 | 630 | 640 | 650 | 660 |
| RLFFIDEVEV | FLGFQNQLRE | SLSLTTMTRD | MRFYNVSGIT | ESDLDEAEIR | IKMAENRDFH |
| 670 | 680 | 690 | 700 | 710 | 720 |
| KWFALWGPWH | KVLERIAPEE | WREMMAKRDE | CIETDEYQSR | VNAELEDLRI | ADDSDAERTT |
| 730 | 740 | 750 | 760 | ||
| EVQMDAERAI | GIKIMEEINQ | TLFTEIMENI | LLKKEVSSLM | SAYWR |