D0FH76
SS
Gene name |
VPS4 |
Protein name |
Vacuolar protein sorting-associated protein 4 |
Names |
EC 3.6.4.6 , BmVps4 , Vacuolar protein sorting 4 |
Species |
Bombyx mori (Silk moth) |
KEGG Pathway |
bmor:100313493 |
EC number |
3.6.4.6: Acting on ATP; involved in cellular and subcellular movement |
Protein Class |
|
Descriptions
VPS4 proteins are AAA+ ATPases required to form multivesicular bodies, release viral particles, and complete cytokinesis. VPS4 proteins act by disassembling ESCRT-III heteropolymers during or after their proposed function in membrane scission. Deleting the N-terminal MIT domain and adjacent linker from VPS4A increases both basal and liposome-enhanced ATPase activity, indicating that these elements play a role in autoinhibiting VPS4A until it encounters ESCRT-III polymers. The interactions between acidic ESCRT-III residues and sequences in VPS4A, in particular in the linker connecting the MIT and AAA+ domains, are involved in regulating the intrinsic autoinhibition of the enzyme.
Autoinhibitory domains (AIDs)
Target domain |
160-296 (AAA+ domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for D0FH76
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-D0FH76-F1 | Predicted | AlphaFoldDB |
No variants for D0FH76
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for D0FH76 | |||||
1 associated diseases with D0FH76
[MIM: 618283]: Visual impairment and progressive phthisis bulbi (VIPB)
An autosomal recessive, progressive disease characterized by poor vision at birth and development of bilateral phthisis bulbi by adulthood. . Note=The disease may be caused by variants affecting the gene represented in this entry.
Without disease ID
- An autosomal recessive, progressive disease characterized by poor vision at birth and development of bilateral phthisis bulbi by adulthood. . Note=The disease may be caused by variants affecting the gene represented in this entry.
Functions
| Description | ||
|---|---|---|
| EC Number | 3.6.4.6 | Acting on ATP; involved in cellular and subcellular movement |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| endosome membrane | The lipid bilayer surrounding an endosome. |
| late endosome membrane | The lipid bilayer surrounding a late endosome. |
| midbody | A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis. |
| vacuolar membrane | The lipid bilayer surrounding the vacuole and separating its contents from the cytoplasm of the cell. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ATP hydrolysis activity | Catalysis of the reaction |
| ATP-dependent protein disaggregase activity | An ATP-dependent molecular chaperone activity that mediates the solubilization of ordered protein aggregates. |
| protein self-association | Binding to a domain within the same polypeptide. |
9 GO annotations of biological process
| Name | Definition |
|---|---|
| cell cycle | The progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events. Canonically, the cell cycle comprises the replication and segregation of genetic material followed by the division of the cell, but in endocycles or syncytial cells nuclear replication or nuclear division may not be followed by cell division. |
| cell division | The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells. |
| endosomal transport | The directed movement of substances mediated by an endosome, a membrane-bounded organelle that carries materials enclosed in the lumen or located in the endosomal membrane. |
| instar larval or pupal development | The process whose specific outcome is the progression of the instar larva or pupa over time, from its formation to the mature structure. An example of this process is found in Drosophila melanogaster. |
| metamorphosis | A biological process in which an animal physically develops after birth or hatching, involving a conspicuous and relatively abrupt change in the animal's form or structure. Examples include the change from tadpole to frog, and the change from larva to adult. An example of this is found in Drosophila melanogaster. |
| protein complex oligomerization | The process of creating protein oligomers, compounds composed of a small number, usually between three and ten, of component monomers; protein oligomers may be composed of different or identical monomers. Oligomers may be formed by the polymerization of a number of monomers or the depolymerization of a large protein polymer. |
| protein transport | The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. |
| ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | The chemical reactions and pathways resulting in the breakdown of a protein or peptide covalently tagged with ubiquitin, via the multivesicular body (MVB) sorting pathway; ubiquitin-tagged proteins are sorted into MVBs, and delivered to a lysosome/vacuole for degradation. |
| vacuole organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a vacuole. |
7 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q0VD48 | VPS4B | Vacuolar protein sorting-associated protein 4B | Bos taurus (Bovine) | SS |
| O75351 | VPS4B | Vacuolar protein sorting-associated protein 4B | Homo sapiens (Human) | PR |
| Q9UN37 | VPS4A | Vacuolar protein sorting-associated protein 4A | Homo sapiens (Human) | EV |
| P46467 | Vps4b | Vacuolar protein sorting-associated protein 4B | Mus musculus (Mouse) | PR |
| Q8VEJ9 | Vps4a | Vacuolar protein sorting-associated protein 4A | Mus musculus (Mouse) | PR |
| Q793F9 | Vps4a | Vacuolar protein sorting-associated protein 4A | Rattus norvegicus (Rat) | PR |
| Q9ZNT0 | SKD1 | Protein SUPPRESSOR OF K(+) TRANSPORT GROWTH DEFECT 1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MTSSNTLQKA | IDLVTKATEE | DKNKNYEEAL | RLYEHGVEYF | LHAVKYEAQG | ERAKESIRAK |
| 70 | 80 | 90 | 100 | 110 | 120 |
| CLQYLDRAEK | LKEYLKKDQK | KKPVKDGESK | SDDKKSDSDS | DSDDPEKKKL | QGKLEGAIVV |
| 130 | 140 | 150 | 160 | 170 | 180 |
| EKPHVKWSDV | AGLEAAKEAL | KEAVILPIKF | PHLFTGKRIP | WKGILLFGPP | GTGKSYLAKA |
| 190 | 200 | 210 | 220 | 230 | 240 |
| VATEANNSTF | FSVSSSDLVS | KWLGESEKLV | KNLFDLARQH | KPSIIFIDEI | DSLCSSRSDN |
| 250 | 260 | 270 | 280 | 290 | 300 |
| ESESARRIKT | EFLVQMQGVG | NDMDGILVLG | ATNIPWVLDS | AIRRRFEKRI | YIALPEEHAR |
| 310 | 320 | 330 | 340 | 350 | 360 |
| LDMFKLHLGN | TRHQLSEQDM | KLLAAKSEGY | SGADISIVVR | DALMQPVRKV | QSATHFKKIS |
| 370 | 380 | 390 | 400 | 410 | 420 |
| GPSPTDPNVI | VNDLLTPCSP | GDPGAIEMTW | IDVPSDKLGE | PPVTMSDMLR | SLAVSKPTVN |
| 430 | |||||
| DDDMVKLRKF | MEDFGQEG |