B6CJY5

SS
Gene name
TRAF6
Protein name
TNF receptor-associated factor 6
Names
E3 ubiquitin-protein ligase TRAF6, RING-type E3 ubiquitin transferase TRAF6
Species
Macaca mulatta (Rhesus macaque)
KEGG Pathway
mcc:716907
EC number
2.3.2.27: Aminoacyltransferases
Protein Class

Descriptions

The Tumor Necrosis Factor (TNF) Receptor Associated Factor (TRAF) class of intracellular signal transducers is responsible for mediating many of the activation events initiated by TNF receptor (TNFR) and Toll-like/Interleukin-1, 17 and 18 receptor (TIR) families. ​​These receptors can, both directly and indirectly, activate TRAF6 via specific binding of proteins containing TRAF Interacting Motif (TIM) peptides to a groove on the carboxyl-terminal MATH domain. <br>TRAF6 is autoinhibited by an intramolecular interaction between the RING-Zinc (RZ) region and MATH domain. The interaction renders TRAF6 inactive and structurally closed, as well as incapable of auto-ubiquitination. RING-Zinc fingers region and the MATH domain are targets for ubiquitination. TRAF autoubiquitination in trans is a means of sustaining an open conformation active in downstream signaling.

Autoinhibitory domains (AIDs)

346-522 (MATH domain) SS

Target domain

1-273 (RZ domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for B6CJY5

Entry ID Method Resolution Chain Position Source
AF-B6CJY5-F1 Predicted AlphaFoldDB

2 variants for B6CJY5

Variant ID(s) Position Change Description Diseaes Association Provenance
rs1081332673 270 M>L No Ensembl
rs1066712392 335 R>Q No Ensembl

No associated diseases with B6CJY5

2 regional properties for B6CJY5

Type Name Position InterPro Accession
domain CARD domain 6 - 98 IPR001315
domain CARD9, CARD domain 10 - 95 IPR042142

Functions

Description
EC Number 2.3.2.27 Aminoacyltransferases
Subcellular Localization
  • Cytoplasm
  • Cytoplasm, cell cortex
  • Nucleus
  • Lipid droplet
  • RSAD2/viperin recruits it to the lipid droplet
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
CD40 receptor complex A protein complex that contains at least CD40 (a cell surface receptor of the tumour necrosis factor receptor (TNFR) superfamily), and other signaling molecules.
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
cytoplasmic side of plasma membrane The leaflet the plasma membrane that faces the cytoplasm and any proteins embedded or anchored in it or attached to its surface.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
lipid droplet An intracellular non-membrane-bounded organelle comprising a matrix of coalesced lipids surrounded by a phospholipid monolayer. May include associated proteins.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.

13 GO annotations of molecular function

Name Definition
histone deacetylase binding Binding to histone deacetylase.
identical protein binding Binding to an identical protein or proteins.
mitogen-activated protein kinase kinase kinase binding Binding to a mitogen-activated protein kinase kinase kinase, a protein that can phosphorylate a MAP kinase kinase.
protein kinase B binding Binding to protein kinase B, an intracellular kinase that is important in regulating glucose metabolism.
protein N-terminus binding Binding to a protein N-terminus, the end of any peptide chain at which the 2-amino (or 2-imino) function of a constituent amino acid is not attached in peptide linkage to another amino-acid residue.
thioesterase binding Binding to a thioesterase.
tumor necrosis factor receptor binding Binding to a tumor necrosis factor receptor.
tumor necrosis factor receptor superfamily binding Binding to a member of the tumor necrosis factor receptor superfamily.
ubiquitin conjugating enzyme binding Binding to a ubiquitin conjugating enzyme, any of the E2 proteins.
ubiquitin protein ligase activity Catalysis of the transfer of ubiquitin to a substrate protein via the reaction X-ubiquitin + S -> X + S-ubiquitin, where X is either an E2 or E3 enzyme, the X-ubiquitin linkage is a thioester bond, and the S-ubiquitin linkage is an amide bond: an isopeptide bond between the C-terminal glycine of ubiquitin and the epsilon-amino group of lysine residues in the substrate or, in the linear extension of ubiquitin chains, a peptide bond the between the C-terminal glycine and N-terminal methionine of ubiquitin residues.
ubiquitin protein ligase binding Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins.
ubiquitin-protein transferase activity Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages.
zinc ion binding Binding to a zinc ion (Zn).

39 GO annotations of biological process

Name Definition
activation of NF-kappaB-inducing kinase activity The stimulation of the activity of NF-kappaB-inducing kinase through phosphorylation at specific residues.
antigen processing and presentation of exogenous peptide antigen via MHC class II The process in which an antigen-presenting cell expresses a peptide antigen of exogenous origin on its cell surface in association with an MHC class II protein complex. The peptide antigen is typically, but not always, processed from a whole protein.
bone resorption The process in which specialized cells known as osteoclasts degrade the organic and inorganic portions of bone, and endocytose and transport the degradation products.
cell development The process whose specific outcome is the progression of the cell over time, from its formation to the mature structure. Cell development does not include the steps involved in committing a cell to a specific fate.
cellular response to DNA damage stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism.
cellular response to lipopolysaccharide Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipopolysaccharide stimulus; lipopolysaccharide is a major component of the cell wall of gram-negative bacteria.
I-kappaB kinase/NF-kappaB signaling The process in which a signal is passed on to downstream components within the cell through the I-kappaB-kinase (IKK)-dependent activation of NF-kappaB. The cascade begins with activation of a trimeric IKK complex (consisting of catalytic kinase subunits IKKalpha and/or IKKbeta, and the regulatory scaffold protein NEMO) and ends with the regulation of transcription of target genes by NF-kappaB. In a resting state, NF-kappaB dimers are bound to I-kappaB proteins, sequestering NF-kappaB in the cytoplasm. Phosphorylation of I-kappaB targets I-kappaB for ubiquitination and proteasomal degradation, thus releasing the NF-kappaB dimers, which can translocate to the nucleus to bind DNA and regulate transcription.
in utero embryonic development The process whose specific outcome is the progression of the embryo in the uterus over time, from formation of the zygote in the oviduct, to birth. An example of this process is found in Mus musculus.
interleukin-1-mediated signaling pathway The series of molecular signals initiated by interleukin-1 binding to its receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription.
interleukin-17-mediated signaling pathway The series of molecular signals initiated by interleukin-17 binding to its receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription.
myeloid dendritic cell differentiation The process in which a monocyte acquires the specialized features of a dendritic cell, an immunocompetent cell of the lymphoid and hemopoietic systems and skin.
negative regulation of transcription by RNA polymerase II Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II.
neural tube closure The last step in the formation of the neural tube, where the paired neural folds are brought together and fuse at the dorsal midline.
odontogenesis of dentin-containing tooth The process whose specific outcome is the progression of a dentin-containing tooth over time, from its formation to the mature structure. A dentin-containing tooth is a hard, bony organ borne on the jaw or other bone of a vertebrate, and is composed mainly of dentin, a dense calcified substance, covered by a layer of enamel.
ossification The formation of bone or of a bony substance, or the conversion of fibrous tissue or of cartilage into bone or a bony substance.
osteoclast differentiation The process in which a relatively unspecialized monocyte acquires the specialized features of an osteoclast. An osteoclast is a specialized phagocytic cell associated with the absorption and removal of the mineralized matrix of bone tissue.
positive regulation of I-kappaB kinase/NF-kappaB signaling Any process that activates or increases the frequency, rate or extent of I-kappaB kinase/NF-kappaB signaling.
positive regulation of interleukin-12 production Any process that activates or increases the frequency, rate, or extent of interleukin-12 production.
positive regulation of interleukin-2 production Any process that activates or increases the frequency, rate, or extent of interleukin-2 production.
positive regulation of interleukin-6 production Any process that activates or increases the frequency, rate, or extent of interleukin-6 production.
positive regulation of JNK cascade Any process that activates or increases the frequency, rate or extent of signal transduction mediated by the JNK cascade.
positive regulation of JUN kinase activity Any process that activates or increases the frequency, rate or extent of JUN kinase activity.
positive regulation of leukocyte adhesion to vascular endothelial cell Any process that activates or increases the frequency, rate or extent of leukocyte adhesion to vascular endothelial cell.
positive regulation of lipopolysaccharide-mediated signaling pathway Any process that activates or increases the frequency, rate or extent of signaling in response to detection of lipopolysaccharide.
positive regulation of NF-kappaB transcription factor activity Any process that activates or increases the frequency, rate or extent of activity of the transcription factor NF-kappaB.
positive regulation of NIK/NF-kappaB signaling Any process that activates or increases the frequency, rate or extent of NIK/NF-kappaB signaling.
positive regulation of osteoclast differentiation Any process that activates or increases the frequency, rate or extent of osteoclast differentiation.
positive regulation of T cell cytokine production Any process that activates or increases the frequency, rate, or extent of T cell cytokine production.
positive regulation of T cell proliferation Any process that activates or increases the rate or extent of T cell proliferation.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
positive regulation of transcription regulatory region DNA binding Any process that activates or increases the frequency, rate or extent of transcription regulatory region DNA binding.
protein autoubiquitination The ubiquitination by a protein of one or more of its own amino acid residues, or residues on an identical protein. Ubiquitination occurs on the lysine residue by formation of an isopeptide crosslink.
protein K63-linked ubiquitination A protein ubiquitination process in which a polymer of ubiquitin, formed by linkages between lysine residues at position 63 of the ubiquitin monomers, is added to a protein. K63-linked ubiquitination does not target the substrate protein for degradation, but is involved in several pathways, notably as a signal to promote error-free DNA postreplication repair.
regulation of apoptotic process Any process that modulates the occurrence or rate of cell death by apoptotic process.
regulation of I-kappaB kinase/NF-kappaB signaling Any process that modulates I-kappaB kinase/NF-kappaB signaling.
regulation of immunoglobulin production Any process that modulates the frequency, rate, or extent of immunoglobulin production.
T cell receptor signaling pathway The series of molecular signals initiated by the cross-linking of an antigen receptor on a T cell.
T-helper 1 type immune response An immune response which is associated with resistance to intracellular bacteria, fungi, and protozoa, and pathological conditions such as arthritis, and which is typically orchestrated by the production of particular cytokines by T-helper 1 cells, most notably interferon-gamma, IL-2, and lymphotoxin.
tumor necrosis factor-mediated signaling pathway The series of molecular signals initiated by tumor necrosis factor binding to its receptor on the surface of a cell, and ending with the regulation of a downstream cellular process, e.g. transcription.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q3ZCC3 TRAF6 TNF receptor-associated factor 6 Bos taurus (Bovine) SS
Q9Y4K3 TRAF6 TNF receptor-associated factor 6 Homo sapiens (Human) EV
P70196 Traf6 TNF receptor-associated factor 6 Mus musculus (Mouse) SS
A7XUJ6 TRAF6 TNF receptor-associated factor 6 Sus scrofa (Pig) SS
B5DF45 Traf6 TNF receptor-associated factor 6 Rattus norvegicus (Rat) SS
Q28DL4 traf6 TNF receptor-associated factor 6 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
Q6IWL4 traf6 TNF receptor-associated factor 6 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MSLLNCENSC GSSQSESDCC VAMASSCSAA TKDDSVGGTA STGNLSSSFM EDIQGYDVEF
70 80 90 100 110 120
DPPLESKYEC PICLMALREA VQTPCGHRFC KACIIKSIRD AGHKCPVDNE ILLENQLFPD
130 140 150 160 170 180
NFAKREILSL MVKCPNEGCL HKMELRHLED HQAHCEFALV DCPQCQRPFQ KFHINIHILK
190 200 210 220 230 240
DCPRRQVSCD NCAALVAFED KEIHDQNCPL ANVICEYCNT ILIREQMPNH YDLDCPTAPI
250 260 270 280 290 300
PCTFSTFGCH EKMQRNHLAR HLQENTQSHM RMLAQAVHSL SLIPDSGYVS EVRNFQETIH
310 320 330 340 350 360
QLEGRLVRQD HQIRELTAKM ETQSTYVSEL KRTIRTLEDK VAEIEAQQCN GIYIWKIGNF
370 380 390 400 410 420
GMHLKCQEEE KPVVIHSPGF YTGKPGYKLC MRLHLQLPTA QRCANYISLF VHTMQGEYDS
430 440 450 460 470 480
HLPWPFQGTI RLTILDQSEA PVRQNHEEIM DAKPDLLAFQ RPTIPRNPKG FGYVTFMHLE
490 500 510 520
ALRQRTFIKD DTLLVRCEVS TRFDMGSLRR EGFQPRSTDS GV