B2DD29

SS
Gene name
Brsk1 (Sadb)
Protein name
Serine/threonine-protein kinase BRSK1
Names
EC 2.7.11.1 , EC 2.7.11.26 , Brain-specific serine/threonine-protein kinase 1 , BR serine/threonine-protein kinase 1 , Serine/threonine-protein kinase SAD-B
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:499073
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

SAD-B is a serine/threonine kinase involved in neuronal development, cell cycle, and energy metabolism. It is highly expressed in the brain and plays a role in axonal arborization, nerve terminal maturation, and neurotransmitter release. SAD-B contains an AIS near the KA1 domain that regulates its activity. This sequence is flexible and solvent-accessible, allowing it to interact with the kinase domain (KD) and ubiquitin-associated (UBA) domain to inhibit SAD-B activity. The crystal structure of the mouse SAD-B AIS-KA1 fragment reveals that the AIS is highly flexible in its isolated state, suggesting its readiness to bind to the KD-UBA junction for autoinhibition. Furthermore, the biochemical experiments demonstrate that the AIS of SAD-B non-competitively inhibits the kinase activity of SAD-B, similar to the autoinhibition observed in SAD-A, indicating a conserved mechanism between these kinases.

Autoinhibitory domains (AIDs)

592-603 (Autoinhibitory sequence, AIS) SS

Target domain

34-356 (KD-UBA domains)

Relief mechanism

Ligand binding, Others

Assay

302-358 (UBA domain) SS

Target domain

604-715 (KA1 domain)

Relief mechanism

Ligand binding, Others

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

0 structures for B2DD29

Entry ID Method Resolution Chain Position Source
No available structures

No variants for B2DD29

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for B2DD29

No associated diseases with B2DD29

2 regional properties for B2DD29

Type Name Position InterPro Accession
domain ABC1 atypical kinase-like domain 180 - 419 IPR004147
domain ADCK3-like domain 176 - 425 IPR034646

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Nucleus
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Synapse
  • Presynaptic active zone
  • Cytoplasmic vesicle, secretory vesicle, synaptic vesicle
  • Nuclear in the absence of DNA damage
  • Translocated to the nucleus in response to UV- or MMS-induced DNA damage (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
distal axon That part of an axon close to and including the growth cone or the axon terminus.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
presynaptic active zone A specialized region of the plasma membrane and cell cortex of a presynaptic neuron; encompasses a region of the plasma membrane where synaptic vesicles dock and fuse, and a specialized cortical cytoskeletal matrix.
synaptic vesicle A secretory organelle, typically 50 nm in diameter, of presynaptic nerve terminals; accumulates in high concentrations of neurotransmitters and secretes these into the synaptic cleft by fusion with the 'active zone' of the presynaptic plasma membrane.

8 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
gamma-tubulin binding Binding to the microtubule constituent protein gamma-tubulin.
magnesium ion binding Binding to a magnesium (Mg) ion.
molecular function inhibitor activity A molecular function regulator that inhibits or decreases the activity of its target via non-covalent binding that does not result in covalent modification to the target.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions
tau-protein kinase activity Catalysis of the reaction

17 GO annotations of biological process

Name Definition
associative learning Learning by associating a stimulus (the cause) with a particular outcome (the effect).
axonogenesis De novo generation of a long process of a neuron, including the terminal branched region. Refers to the morphogenesis or creation of shape or form of the developing axon, which carries efferent (outgoing) action potentials from the cell body towards target cells.
centrosome duplication The replication of a centrosome, a structure comprised of a pair of centrioles and peri-centriolar material from which a microtubule spindle apparatus is organized.
DNA damage response Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism.
establishment of cell polarity The specification and formation of anisotropic intracellular organization or cell growth patterns.
G2/M transition of mitotic cell cycle The mitotic cell cycle transition by which a cell in G2 commits to M phase. The process begins when the kinase activity of M cyclin/CDK complex reaches a threshold high enough for the cell cycle to proceed. This is accomplished by activating a positive feedback loop that results in the accumulation of unphosphorylated and active M cyclin/CDK complex.
microtubule cytoskeleton organization involved in establishment of planar polarity A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising microtubules and their associated proteins and contributes to the establishment of planar polarity.
mitotic G2 DNA damage checkpoint signaling A mitotic cell cycle checkpoint that detects and negatively regulates progression through the G2/M transition of the cell cycle in response to DNA damage.
neuron differentiation The process in which a relatively unspecialized cell acquires specialized features of a neuron.
neuron projection morphogenesis The process in which the anatomical structures of a neuron projection are generated and organized. A neuron projection is any process extending from a neural cell, such as axons or dendrites.
neurotransmitter secretion The regulated release of neurotransmitter from the presynapse into the synaptic cleft via calcium-regulated exocytosis during synaptic transmission.
protein autophosphorylation The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation).
regulation of axonogenesis Any process that modulates the frequency, rate or extent of axonogenesis, the generation of an axon, the long process of a neuron.
regulation of neuron projection development Any process that modulates the rate, frequency or extent of neuron projection development. Neuron projection development is the process whose specific outcome is the progression of a neuron projection over time, from its formation to the mature structure. A neuron projection is any process extending from a neural cell, such as axons or dendrites (collectively called neurites).
regulation of synaptic plasticity A process that modulates synaptic plasticity, the ability of synapses to change as circumstances require. They may alter function, such as increasing or decreasing their sensitivity, or they may increase or decrease in actual numbers.
response to UV Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ultraviolet radiation (UV light) stimulus. Ultraviolet radiation is electromagnetic radiation with a wavelength in the range of 10 to 380 nanometers.
synaptic vesicle cycle A biological process in which synaptic vesicles are loaded with neurotransmitters, move to the active zone, exocytose and are then recycled via endocytosis, ultimately leading to reloading with neurotransmitters.

12 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q8IWQ3 BRSK2 Serine/threonine-protein kinase BRSK2 Homo sapiens (Human) PR
Q8TDC3 BRSK1 Serine/threonine-protein kinase BRSK1 Homo sapiens (Human) SS
Q69Z98 Brsk2 Serine/threonine-protein kinase BRSK2 Mus musculus (Mouse) EV
Q5RJI5 Brsk1 Serine/threonine-protein kinase BRSK1 Mus musculus (Mouse) EV
Q91ZN7 Chek1 Serine/threonine-protein kinase Chk1 Rattus norvegicus (Rat) PR
P54645 Prkaa1 5'-AMP-activated protein kinase catalytic subunit alpha-1 Rattus norvegicus (Rat) EV
Q09137 Prkaa2 5'-AMP-activated protein kinase catalytic subunit alpha-2 Rattus norvegicus (Rat) EV
D3ZML2 Brsk2 Serine/threonine-protein kinase BRSK2 Rattus norvegicus (Rat) SS
Q5JLQ9 CIPK30 CBL-interacting protein kinase 30 Oryza sativa subsp japonica (Rice) PR
Q6ERS4 CIPK16 CBL-interacting protein kinase 16 Oryza sativa subsp japonica (Rice) PR
Q6ZLP5 CIPK23 CBL-interacting protein kinase 23 Oryza sativa subsp japonica (Rice) PR
Q19469 sad-1 Serine/threonine kinase SAD-1 Caenorhabditis elegans SS
10 20 30 40 50 60
MSSGSKEGGG GSPAYHLPHP HPHPPQHAQY VGPYRLEKTL GKGQTGLVKL GVHCITGQKV
70 80 90 100 110 120
AVKIVNREKL SESVLMKVER EIAILKLIEH PHVLKLHDVY ENKKYLYLVL EHVSGGELFD
130 140 150 160 170 180
YLVKKGRLTP KEARKFFRQI VSALDFCHSY SICHRDLKPE NLLLDEKNNI RIADFGMASL
190 200 210 220 230 240
QVGDSLLETS CGSPHYACPE VIKGEKYDGR RADMWSCGVI LFALLVGALP FDDDNLRQLL
250 260 270 280 290 300
EKVKRGVFHM PHFIPPDCQS LLRGMIEVEP EKRLSLEQIQ KHPWYLGGKH EPDPCLEPAP
310 320 330 340 350 360
GRRVAMRSLP SNGELDPDVL ESMASLGCFR DRERLHRELR SEEENQEKMI YYLLLDRKER
370 380 390 400 410 420
YPSCEDQDLP PRNDVDPPRK RVDSPMLSRH GKRRPERKSM EVLSITDAGS GGSPVPTRRA
430 440 450 460 470 480
LEMAQHSQRS RSVSGASTGL SSSPLSSPRS PVFSFSPEPG VGDEARGGGS PTSKTQTLPS
490 500 510 520 530 540
RGPRGGGAGE QPPPPSARST PLPGPPGSPR SSGGTPLHSP LHTPRASPTG TPGTTPPPSP
550 560 570 580 590 600
GGGVGGAAWR SRLNSIRNSF LGSPRFHRRK MQVPTAEEMS SLTPESSPEL AKRSWFGNFI
610 620 630 640 650 660
SLDKEEQIFL VLKDKPLSSI KADIVHAFLS IPSLSHSVLS QTSFRAEYKA SGGPSVFQKP
670 680 690 700 710 720
VRFQVDISSS EGPEPSPRRD GSSGGGIYSV TFTLISGPSR RFKRVVETIQ AQLLSTHDQP
730 740 750 760 770
SVQALADEKN GAQTRPAGTP PRSLQPPPGR PDPDLSSSPR RGPSKDKKLL ATNGTPLP