A8MVU1

SS
Gene name
NCF1C (SH3PXD1C)
Protein name
Putative neutrophil cytosol factor 1C
Names
NCF-1C , Putative SH3 and PX domain-containing protein 1C
Species
Homo sapiens (Human)
KEGG Pathway
EC number
Protein Class

Descriptions

Phagocyte NADPH oxidase is a multisubunit enzyme responsible for the production of reactive oxygen species. NCF1 (p47phox) is a cytosolic component of the NADPH oxidase and plays an important role in the assembly of the activated complex. NCF1 is composed of several domains including a PX domain, tandem SH3 domains (N-SH3 and C-SH3), a polybasic/autoinhibitory region (PBR/AIR), and a proline-rich region (PRR). In an inactive state, the binding surface of the tandem SH3 domains for the binding to the PRR region of p22phox (CYBA) is masked through the intramolecular interaction with PBR/AIR. Phosphorylations of the Ser residues in PBR/AIR by PKC or Akt cause a conformational change in NCF1 and activation of the NADPH oxidase.

Autoinhibitory domains (AIDs)

263-305 (Polybasic and autoinhibitory region) SS

Target domain

135-188 (N-SH3);205-258 (C-SH3)

Relief mechanism

PTM

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for A8MVU1

Entry ID Method Resolution Chain Position Source
AF-A8MVU1-F1 Predicted AlphaFoldDB

No variants for A8MVU1

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for A8MVU1

No associated diseases with A8MVU1

8 regional properties for A8MVU1

Type Name Position InterPro Accession
repeat WD40 repeat 1336 - 1414 IPR001680-1
repeat WD40 repeat 1440 - 1478 IPR001680-2
repeat WD40 repeat 1481 - 1523 IPR001680-3
repeat WD40 repeat 1531 - 1615 IPR001680-4
repeat WD40 repeat 1615 - 1655 IPR001680-5
domain Kinesin motor domain 7 - 379 IPR001752
conserved_site WD40 repeat, conserved site 1360 - 1374 IPR019775
conserved_site Kinesin motor domain, conserved site 268 - 279 IPR019821

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
NADPH oxidase complex A enzyme complex of which the core is a heterodimer composed of a light (alpha) and heavy (beta) chain, and requires several other water-soluble proteins of cytosolic origin for activity. Functions in superoxide generation by the NADPH-dependent reduction of O2.

2 GO annotations of molecular function

Name Definition
phosphatidylinositol binding Binding to an inositol-containing glycerophospholipid, i.e. phosphatidylinositol (PtdIns) and its phosphorylated derivatives.
superoxide-generating NADPH oxidase activator activity Binds to and increases the activity of the enzyme superoxide-generating NADPH oxidase.

2 GO annotations of biological process

Name Definition
respiratory burst A phase of elevated metabolic activity, during which oxygen consumption increases; this leads to the production, by an NADH dependent system, of hydrogen peroxide (H2O2), superoxide anions and hydroxyl radicals.
superoxide anion generation The enzymatic generation of superoxide, the superoxide anion O2- (superoxide free radical), or any compound containing this species, by a cell in response to environmental stress, thereby mediating the activation of various stress-inducible signaling pathways.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
O77774 NCF1 Neutrophil cytosol factor 1 Bos taurus (Bovine) SS
A6NI72 NCF1B Putative neutrophil cytosol factor 1B Homo sapiens (Human) SS
P14598 NCF1 Neutrophil cytosol factor 1 Homo sapiens (Human) EV
A1X283 SH3PXD2B SH3 and PX domain-containing protein 2B Homo sapiens (Human) EV
Q5TCZ1 SH3PXD2A SH3 and PX domain-containing protein 2A Homo sapiens (Human) SS
Q09014 Ncf1 Neutrophil cytosol factor 1 Mus musculus (Mouse) SS
F1M707 Ncf1 Neutrophil cytosolic factor 1 Rattus norvegicus (Rat) SS
10 20 30 40 50 60
MYMFLVKWQD LSEKVVYRRF TEIYEFHKTL KEMFPIEAGA INPENRIIPH LPAPKWFDGQ
70 80 90 100 110 120
RAAENHQGTL TEYCSTLMSL PTKISRCPHL LDFFKVRPDD LKLPTDNQTK KPETYLMPKD
130 140 150 160 170 180
GKSTATDITG PIILQTYRAI ADYEKTSGSE MALSTGDVVE VVEKSESGWW FCQMKAKRGW
190 200 210 220 230 240
IPASFLEPLD SPDETEDPEP NYAGEPYVAI KAYTAVEGDE VSLLEGEAVE VIHKLLDGWW
250 260 270 280 290 300
VIRKDDVTGY FPSMYLQKSG QDVSQAQRQI KRGAPPRRSS IRNAHSIHQR SRKRLSQDAY
310 320 330 340 350 360
RRNSVRFLQQ RRRQARPGPQ SPGSPLEEER QTQRSKPQPA VPPRPSADLI LNRCSESTKR
KLASAV