A8C984

SS
Gene name
mylk3 (mlck)
Protein name
Myosin light chain kinase 3
Names
EC 2.7.11.18 , Cardiac-MyBP-C-associated Ca/CaM kinase , Cardiac-MLCK
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
KEGG Pathway
dre:561635
EC number
2.7.11.18: Protein-serine/threonine kinases
Protein Class

Descriptions

Myosin light chain kinases (MLCK) are members of the family of Ca2+-calmodulin-dependent protein kinases. This enzyme contains an autoinhibitory pseudosubstrate sequence that blocks the substrate-binding site of the kinase. The autoinhibitory sequence makes an extensive network of contacts on the surface of the larger C-domain of the catalytic core extending towards the catalytic cleft with the CaM-binding sequence to block the regulatory light chain of sarcomeric myosin, but not ATP binding in the catalytic core N-domain.

Autoinhibitory domains (AIDs)

656-684 (Autoinhibitory pseudosubstrate) SS

Target domain

404-659 (Protein kinase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for A8C984

Entry ID Method Resolution Chain Position Source
AF-A8C984-F1 Predicted AlphaFoldDB

No variants for A8C984

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for A8C984

No associated diseases with A8C984

3 regional properties for A8C984

Type Name Position InterPro Accession
domain Protein kinase domain 404 - 659 IPR000719
active_site Serine/threonine-protein kinase, active site 521 - 533 IPR008271
binding_site Protein kinase, ATP binding site 410 - 433 IPR017441

Functions

Description
EC Number 2.7.11.18 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

2 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
myosin light chain kinase activity Catalysis of the reaction

6 GO annotations of biological process

Name Definition
heart morphogenesis The developmental process in which the heart is generated and organized. The heart is a hollow, muscular organ, which, by contracting rhythmically, keeps up the circulation of the blood.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
positive regulation of apoptotic process Any process that activates or increases the frequency, rate or extent of cell death by apoptotic process.
sarcomerogenesis The process in which sarcomeres are added in series within a fiber.
ventricular cardiac myofibril assembly The process whose specific outcome is the progression of the ventricular cardiac myofibril over time, from its formation to the mature structure. A cardiac myofibril is a myofibril specific to cardiac muscle cells.

101 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
A4IFM7 MYLK2 Myosin light chain kinase 2, skeletal/cardiac muscle Bos taurus (Bovine) SS
Q0V7M1 PSKH1 Serine/threonine-protein kinase H1 Bos taurus (Bovine) SS
Q0VD22 STK33 Serine/threonine-protein kinase 33 Bos taurus (Bovine) PR
Q96NX5 CAMK1G Calcium/calmodulin-dependent protein kinase type 1G Homo sapiens (Human) SS
O15075 DCLK1 Serine/threonine-protein kinase DCLK1 Homo sapiens (Human) EV
Q14012 CAMK1 Calcium/calmodulin-dependent protein kinase type 1 Homo sapiens (Human) EV
Q9UIK4 DAPK2 Death-associated protein kinase 2 Homo sapiens (Human) EV
O94768 STK17B Serine/threonine-protein kinase 17B Homo sapiens (Human) PR
Q86YV6 MYLK4 Myosin light chain kinase family member 4 Homo sapiens (Human) SS
Q16566 CAMK4 Calcium/calmodulin-dependent protein kinase type IV Homo sapiens (Human) SS
P11801 PSKH1 Serine/threonine-protein kinase H1 Homo sapiens (Human) SS
Q8N568 DCLK2 Serine/threonine-protein kinase DCLK2 Homo sapiens (Human) PR
Q9UEE5 STK17A Serine/threonine-protein kinase 17A Homo sapiens (Human) SS
O75962 TRIO Triple functional domain protein Homo sapiens (Human) EV
Q8WWF8 CAPSL Calcyphosin-like protein Homo sapiens (Human) PR
P53355 DAPK1 Death-associated protein kinase 1 Homo sapiens (Human) EV
Q6P2M8 PNCK Calcium/calmodulin-dependent protein kinase type 1B Homo sapiens (Human) SS
Q9BYT3 STK33 Serine/threonine-protein kinase 33 Homo sapiens (Human) PR
Q8NCB2 CAMKV CaM kinase-like vesicle-associated protein Homo sapiens (Human) SS
O43293 DAPK3 Death-associated protein kinase 3 Homo sapiens (Human) PR
Q8IU85 CAMK1D Calcium/calmodulin-dependent protein kinase type 1D Homo sapiens (Human) SS
Q9H1R3 MYLK2 Myosin light chain kinase 2, skeletal/cardiac muscle Homo sapiens (Human) EV
Q32MK0 MYLK3 Myosin light chain kinase 3 Homo sapiens (Human) SS
Q9QYK9 Pnck Calcium/calmodulin-dependent protein kinase type 1B Mus musculus (Mouse) PR
Q91VB2 Camk1g Calcium/calmodulin-dependent protein kinase type 1G Mus musculus (Mouse) SS
Q8BG48 Stk17b Serine/threonine-protein kinase 17B Mus musculus (Mouse) PR
Q8VCR8 Mylk2 Myosin light chain kinase 2, skeletal/cardiac muscle Mus musculus (Mouse) SS
Q6PGN3 Dclk2 Serine/threonine-protein kinase DCLK2 Mus musculus (Mouse) PR
Q91YS8 Camk1 Calcium/calmodulin-dependent protein kinase type 1 Mus musculus (Mouse) SS
Q8VDF3 Dapk2 Death-associated protein kinase 2 Mus musculus (Mouse) EV
Q6P8Y1 Capsl Calcyphosin-like protein Mus musculus (Mouse) PR
Q8BW96 Camk1d Calcium/calmodulin-dependent protein kinase type 1D Mus musculus (Mouse) SS
Q3UHL1 Camkv CaM kinase-like vesicle-associated protein Mus musculus (Mouse) SS
P08414 Camk4 Calcium/calmodulin-dependent protein kinase type IV Mus musculus (Mouse) SS
Q9JLM8 Dclk1 Serine/threonine-protein kinase DCLK1 Mus musculus (Mouse) SS
Q91YA2 Pskh1 Serine/threonine-protein kinase H1 Mus musculus (Mouse) SS
Q80YE7 Dapk1 Death-associated protein kinase 1 Mus musculus (Mouse) SS
Q62407 Speg Striated muscle-specific serine/threonine-protein kinase Mus musculus (Mouse) SS
Q0KL02 Trio Triple functional domain protein Mus musculus (Mouse) SS
Q924X7 Stk33 Serine/threonine-protein kinase 33 Mus musculus (Mouse) PR
O54784 Dapk3 Death-associated protein kinase 3 Mus musculus (Mouse) PR
Q3UIZ8 Mylk3 Myosin light chain kinase 3 Mus musculus (Mouse) SS
Q7TNJ7 Camk1g Calcium/calmodulin-dependent protein kinase type 1G Rattus norvegicus (Rat) SS
P20689 Mylk2 Myosin light chain kinase 2, skeletal/cardiac muscle Rattus norvegicus (Rat) SS
F1M0Z1 Trio Triple functional domain protein Rattus norvegicus (Rat) SS
Q91XS8 Stk17b Serine/threonine-protein kinase 17B Rattus norvegicus (Rat) PR
O08875 Dclk1 Serine/threonine-protein kinase DCLK1 Rattus norvegicus (Rat) PR
O70150 Pnck Calcium/calmodulin-dependent protein kinase type 1B Rattus norvegicus (Rat) PR
Q63092 Camkv CaM kinase-like vesicle-associated protein Rattus norvegicus (Rat) SS
Q63638 Speg Striated muscle-specific serine/threonine-protein kinase Rattus norvegicus (Rat) SS
O88764 Dapk3 Death-associated protein kinase 3 Rattus norvegicus (Rat) PR
P13234 Camk4 Calcium/calmodulin-dependent protein kinase type IV Rattus norvegicus (Rat) SS
Q63450 Camk1 Calcium/calmodulin-dependent protein kinase type 1 Rattus norvegicus (Rat) EV
P97924 Kalrn Kalirin Rattus norvegicus (Rat) SS
Q5MPA9 Dclk2 Serine/threonine-protein kinase DCLK2 Rattus norvegicus (Rat) PR
E9PT87 Mylk3 Myosin light chain kinase 3 Rattus norvegicus (Rat) SS
Q5VQQ5 CPK2 Calcium-dependent protein kinase 2 Oryza sativa subsp. japonica (Rice) SS
Q8LPZ7 CPK3 Calcium-dependent protein kinase 3 Oryza sativa subsp. japonica (Rice) SS
Q6K968 CPK6 Calcium-dependent protein kinase 6 Oryza sativa subsp. japonica (Rice) SS
P53684 CPK7 Calcium-dependent protein kinase 7 Oryza sativa subsp japonica (Rice) PR
Q75GE8 CPK8 Calcium-dependent protein kinase 8 Oryza sativa subsp. japonica (Rice) SS
Q6I5I8 CPK16 Calcium-dependent protein kinase 16 Oryza sativa subsp. japonica (Rice) SS
Q6AVM3 CCAMK Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase Oryza sativa subsp japonica (Rice) PR
Q84SL0 CPK20 Calcium-dependent protein kinase 20 Oryza sativa subsp. japonica (Rice) SS
P53682 CPK23 Calcium-dependent protein kinase 23 Oryza sativa subsp japonica (Rice) PR
Q9TXJ0 cmk-1 Calcium/calmodulin-dependent protein kinase type 1 Caenorhabditis elegans PR
O44997 dapk-1 Death-associated protein kinase dapk-1 Caenorhabditis elegans SS
Q95QC4 zyg-8 Serine/threonine-protein kinase zyg-8 Caenorhabditis elegans PR
P28583 Calcium-dependent protein kinase SK5 Glycine max (Soybean) (Glycine hispida) PR
Q9M9V8 CPK10 Calcium-dependent protein kinase 10 Arabidopsis thaliana (Mouse-ear cress) PR
Q39016 CPK11 Calcium-dependent protein kinase 11 Arabidopsis thaliana (Mouse-ear cress) SS
Q9FX86 CRK8 CDPK-related kinase 8 Arabidopsis thaliana (Mouse-ear cress) PR
Q9SSF8 CPK30 Calcium-dependent protein kinase 30 Arabidopsis thaliana (Mouse-ear cress) PR
Q38872 CPK6 Calcium-dependent protein kinase 6 Arabidopsis thaliana (Mouse-ear cress) PR
Q7XJR9 CPK16 Calcium-dependent protein kinase 16 Arabidopsis thaliana (Mouse-ear cress) PR
Q9SIQ7 CPK24 Calcium-dependent protein kinase 24 Arabidopsis thaliana (Mouse-ear cress) PR
Q9SJ61 CPK25 Calcium-dependent protein kinase 25 Arabidopsis thaliana (Mouse-ear cress) PR
Q9ZV15 CPK20 Calcium-dependent protein kinase 20 Arabidopsis thaliana (Mouse-ear cress) PR
P93759 GK-1 Calcium-dependent protein kinase 14 Arabidopsis thaliana (Mouse-ear cress) PR
Q8S8S2 LPEAT2 Lysophospholipid acyltransferase LPEAT2 Arabidopsis thaliana (Mouse-ear cress) PR
Q38870 CPK2 Calcium-dependent protein kinase 2 Arabidopsis thaliana (Mouse-ear cress) PR
Q9LJL9 CRK2 CDPK-related kinase 2 Arabidopsis thaliana (Mouse-ear cress) PR
Q9SG12 CRK6 CDPK-related kinase 6 Arabidopsis thaliana (Mouse-ear cress) PR
Q9SCS2 CRK5 CDPK-related kinase 5 Arabidopsis thaliana (Mouse-ear cress) PR
Q8W4I7 CPK13 Calcium-dependent protein kinase 13 Arabidopsis thaliana (Mouse-ear cress) PR
Q6NLQ6 CPK32 Calcium-dependent protein kinase 32 Arabidopsis thaliana (Mouse-ear cress) PR
Q9ZSA4 CPK27 Calcium-dependent protein kinase 27 Arabidopsis thaliana (Mouse-ear cress) PR
Q38869 CPK4 Calcium-dependent protein kinase 4 Arabidopsis thaliana (Mouse-ear cress) SS
Q42479 CPK3 Calcium-dependent protein kinase 3 Arabidopsis thaliana (Mouse-ear cress) PR
Q1PE17 CPK18 Calcium-dependent protein kinase 18 Arabidopsis thaliana (Mouse-ear cress) SS
Q06850 CPK1 Calcium-dependent protein kinase 1 Arabidopsis thaliana (Mouse-ear cress) PR
Q9FMP5 CPK17 Calcium-dependent protein kinase 17 Arabidopsis thaliana (Mouse-ear cress) PR
Q38873 CPK7 Calcium-dependent protein kinase 7 Arabidopsis thaliana (Mouse-ear cress) PR
Q3E9C0 CPK34 Calcium-dependent protein kinase 34 Arabidopsis thaliana (Mouse-ear cress) PR
Q42438 CPK8 Calcium-dependent protein kinase 8 Arabidopsis thaliana (Mouse-ear cress) PR
Q42396 CPK12 Calcium-dependent protein kinase 12 Arabidopsis thaliana (Mouse-ear cress) SS
Q9FKW4 CPK28 Calcium-dependent protein kinase 28 Arabidopsis thaliana (Mouse-ear cress) SS
Q1LUA6 trio Triple functional domain protein Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q6DEH3 camk2d1 Calcium/calmodulin-dependent protein kinase type II delta 1 chain Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q6DGS3 camk2d2 Calcium/calmodulin-dependent protein kinase type II delta 2 chain Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q7SY49 camkv CaM kinase-like vesicle-associated protein Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MGTSLYRSTL LSTGGFSVSD YIRKFTKNKP DVNNIQPNVG FCHQSTQTSQ QVKEVISAEL
70 80 90 100 110 120
ELQQPEVTLP NDPSSQHSPE AHTGASEPLK PVSAGESSKA LQKAKEISVK SSEPTHVQTF
130 140 150 160 170 180
APAVHLEQID VHNVPKQETN SLVAAPADAK CVIETMTKVE KDIALQQERA LERADDSHVA
190 200 210 220 230 240
NKVFLKSIIP GQQLEKIEDP QQQAEVSLFV DEDSLEKSVP GQHLDKKMEV LQKQAKDLPV
250 260 270 280 290 300
VDEDSLNLSA PGQRQLEEKV DVPEKAEKKI DEAHKKMEEV PRKDELCIEK PVIRHAGIKT
310 320 330 340 350 360
QHEETMGMET TVPKHDESKC PTETYVEKTE NKNADVTLES DKIMICAVSP QNTSLDEDEK
370 380 390 400 410 420
SKAAPLRKVE STLLIIDDSP PLPAPFDHRI VSAKQVPINS YYAVNPVEVL GGGRFGQVHK
430 440 450 460 470 480
CAELSSGLTL AAKIIKVRGM KERDEVKNEI GVMNQLNHVN LIQLYDAFES RTNLTLIMEY
490 500 510 520 530 540
VEGGELFERI IDESYQLTEL DAIVFTRQIC EGVQYLHQQY ILHLDLKPEN ILCVNSTGNQ
550 560 570 580 590 600
IKIIDFGLAR KYRPREKLKV NFGTPEFLAP EVVNYDFVSF PTDMWSVGVI TYMLLSGLSP
610 620 630 640 650 660
FMGDNDAETM NNILHAKWEF DTEAFENVSE EAKDFISSLL VSAKCSRLSA SGCMKHSWLN
670 680 690 700 710
NLEDKAKMYK VRLKSQMMLQ RYLVAHRQWK KHFYAVAAAN RLKRFQQSRS ISTPN