A6NI72

SS
Gene name
NCF1B (SH3PXD1B)
Protein name
Putative neutrophil cytosol factor 1B
Names
NCF-1B , Putative SH3 and PX domain-containing protein 1B
Species
Homo sapiens (Human)
KEGG Pathway
EC number
Protein Class

Descriptions

Phagocyte NADPH oxidase is a multisubunit enzyme responsible for the production of reactive oxygen species. NCF1 (p47phox) is a cytosolic component of the NADPH oxidase and plays an important role in the assembly of the activated complex. NCF1 is composed of several domains including a PX domain, tandem SH3 domains (N-SH3 and C-SH3), a polybasic/autoinhibitory region (PBR/AIR), and a proline-rich region (PRR). In an inactive state, the binding surface of the tandem SH3 domains for the binding to the PRR region of p22phox (CYBA) is masked through the intramolecular interaction with PBR/AIR. Phosphorylations of the Ser residues in PBR/AIR by PKC or Akt cause a conformational change in NCF1 and activation of the NADPH oxidase.

Autoinhibitory domains (AIDs)

288-330 (Polybasic and autoinhibitory region) SS

Target domain

160-213 (N-SH3);230-283 (C-SH3)

Relief mechanism

PTM

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for A6NI72

Entry ID Method Resolution Chain Position Source
AF-A6NI72-F1 Predicted AlphaFoldDB

No variants for A6NI72

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for A6NI72

No associated diseases with A6NI72

2 regional properties for A6NI72

Type Name Position InterPro Accession
domain Rho GTPase-activating protein domain 244 - 431 IPR000198
domain CRAL-TRIO lipid binding domain 63 - 218 IPR001251

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
NADPH oxidase complex A enzyme complex of which the core is a heterodimer composed of a light (alpha) and heavy (beta) chain, and requires several other water-soluble proteins of cytosolic origin for activity. Functions in superoxide generation by the NADPH-dependent reduction of O2.

2 GO annotations of molecular function

Name Definition
phosphatidylinositol binding Binding to an inositol-containing glycerophospholipid, i.e. phosphatidylinositol (PtdIns) and its phosphorylated derivatives.
superoxide-generating NADPH oxidase activator activity Binds to and increases the activity of the enzyme superoxide-generating NADPH oxidase.

2 GO annotations of biological process

Name Definition
respiratory burst A phase of elevated metabolic activity, during which oxygen consumption increases; this leads to the production, by an NADH dependent system, of hydrogen peroxide (H2O2), superoxide anions and hydroxyl radicals.
superoxide anion generation The enzymatic generation of superoxide, the superoxide anion O2- (superoxide free radical), or any compound containing this species, by a cell in response to environmental stress, thereby mediating the activation of various stress-inducible signaling pathways.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
O77774 NCF1 Neutrophil cytosol factor 1 Bos taurus (Bovine) SS
P14598 NCF1 Neutrophil cytosol factor 1 Homo sapiens (Human) EV
A1X283 SH3PXD2B SH3 and PX domain-containing protein 2B Homo sapiens (Human) EV
Q5TCZ1 SH3PXD2A SH3 and PX domain-containing protein 2A Homo sapiens (Human) SS
A8MVU1 NCF1C Putative neutrophil cytosol factor 1C Homo sapiens (Human) SS
Q09014 Ncf1 Neutrophil cytosol factor 1 Mus musculus (Mouse) SS
F1M707 Ncf1 Neutrophil cytosolic factor 1 Rattus norvegicus (Rat) SS
10 20 30 40 50 60
MGDTFIRHIA LLGFEKRFVP SQHYVRYMFL VKWQDLSEKV VYRRFTEIYE FHKTLKEMFP
70 80 90 100 110 120
IEAGAINPEN RIIPHLPAPK WFDGQRAAEN HQGTLTEYCG TLMSLPTKIS RCPHLLDFFK
130 140 150 160 170 180
VRPDDLKLPT DNQTKKPETY LMPKDGKSTA TDITGPIILQ TYRAIANYEK TSGSEMALST
190 200 210 220 230 240
GDVVEVVEKS ESGWWFCQMK AKRGWIPASF LEPLDSPDET EDPEPNYAGE PYVAIKAYTA
250 260 270 280 290 300
VEGDEVSLLE GEAVEVIHKL LDGWWVIRKD DVTGYFPSMY LQKSGQDVSQ AQRQIKRGAP
310 320 330 340 350 360
PRRSSIRNVH SIHQRSRKRL SQDAYRRNSV RFLQQRRRQA RPGPQSPGSP LEEERQTQRS
370 380 390
KPQPAVPPRP SADLILNRCS ESTKRKLASA V