A3KGF7

PR
Gene name
Plcb2
Protein name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
Names
Phosphoinositide phospholipase C-beta-2, Phospholipase C-beta-2, PLC-beta-2
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:18796
EC number
3.1.4.11: Phosphoric diester hydrolases
Protein Class

Descriptions

(Annotation based on sequence homology with Q9NQ66)
PLCB1 catalyzes the hydrolysis of 1-phosphatidylinositol 4,5-bisphosphate into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), and mediates intracellular signaling downstream of G protein-coupled receptors. Autoinhibitory region is a portion of the linker that separates the conserved X and Y boxes comprising the catalytic TIM barrel, at residues 468-539, and occludes the active site of PLCB1. The active site of a PLC isozyme toward phospholipid membranes should force the negatively charged X/Y linker away from the active site, therein relieving autoinhibition of the enzyme.

Autoinhibitory domains (AIDs)

436-558 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for A3KGF7

Entry ID Method Resolution Chain Position Source
AF-A3KGF7-F1 Predicted AlphaFoldDB

65 variants for A3KGF7

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3388579696 27 D>E No EVA
rs3388578596 30 T>I No EVA
rs3388577540 37 I>N No EVA
rs3388581733 40 V>E No EVA
rs3388568061 41 D>E No EVA
rs3388579428 42 P>H No EVA
rs216938738 51 Y>H No EVA
rs3388575017 64 I>S No EVA
rs3388578602 77 K>E No EVA
rs3388577482 99 L>P No EVA
rs3388577504 130 V>L No EVA
rs3388575048 148 L>M No EVA
rs3388574988 175 P>H No EVA
rs3388579479 196 D>Y No EVA
rs3388580061 217 P>H No EVA
rs256817932 230 S>A No EVA
rs3388580093 230 S>Y No EVA
rs3388580011 248 K>N No EVA
rs3388573472 285 Q>R No EVA
rs3388581719 312 Q>* No EVA
rs3388578562 355 C>* No EVA
rs3388568077 404 I>T No EVA
rs223878077 426 S>T No EVA
rs3388574975 477 P>T No EVA
rs3388568034 505 V>M No EVA
rs3388568073 508 E>D No EVA
rs3388568036 543 A>T No EVA
rs3388577512 564 F>I No EVA
rs3388573718 577 F>I No EVA
rs3388579475 628 M>K No EVA
rs3388578549 633 F>I No EVA
rs3388581697 663 R>C No EVA
rs3388581671 703 Y>F No EVA
rs3388583020 767 R>P No EVA
rs3144311 790 A>P No EVA
rs3388568054 805 Y>* No EVA
rs3388582431 820 P>S No EVA
rs3388575022 864 N>D No EVA
rs3149505 867 T>A No EVA
rs3388568052 871 T>A No EVA
rs3388578927 873 A>D No EVA
rs3388582439 875 G>R No EVA
rs3388578554 892 E>V No EVA
rs3144309 894 R>W No EVA
rs3388575409 896 L>M No EVA
rs3388579635 918 A>T No EVA
rs3388580014 931 Q>K No EVA
rs49552524 938 Q>P No EVA
rs264382155 940 A>S No EVA
rs3388578865 941 G>C No EVA
rs212164187 971 R>G No EVA
rs3388573715 974 P>L No EVA
rs3144307 1007 T>I No EVA
rs3388571704 1009 Q>P No EVA
rs3388578869 1021 Q>L No EVA
rs255745385 1058 T>I No EVA
rs3388573727 1064 Q>R No EVA
rs3388577530 1086 M>L No EVA
rs3392122257 1088 E>* No EVA
rs3388573729 1093 H>Q No EVA
rs3388578566 1094 Q>R No EVA
rs3388578590 1135 K>N No EVA
rs3144294 1149 E>K No EVA
rs3388568075 1152 D>G No EVA
rs3388568055 1160 A>V No EVA

No associated diseases with A3KGF7

3 regional properties for A3KGF7

Type Name Position InterPro Accession
domain Protein kinase domain 104 - 395 IPR000719
conserved_site Mitogen-activated protein (MAP) kinase, conserved site 139 - 242 IPR003527
binding_site Protein kinase, ATP binding site 110 - 134 IPR017441

Functions

Description
EC Number 3.1.4.11 Phosphoric diester hydrolases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
G-protein beta/gamma-subunit complex The heterodimer formed by the beta and gamma subunits of a heterotrimeric G protein, which dissociates from the alpha subunit upon guanine nuclotide exchange.
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

5 GO annotations of molecular function

Name Definition
calcium ion binding Binding to a calcium ion (Ca2+).
G-protein beta/gamma-subunit complex binding Binding to a complex of G-protein beta/gamma subunits.
phosphatidylinositol phospholipase C activity Catalysis of the reaction: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1,2-diacylglycerol + 1D-myo-inositol 1,4,5-trisphosphate + H(+).
phospholipase C activity Catalysis of the reaction: a phospholipid + H2O = 1,2-diacylglycerol + a phosphatidate.
phospholipid binding Binding to a phospholipid, a class of lipids containing phosphoric acid as a mono- or diester.

6 GO annotations of biological process

Name Definition
detection of chemical stimulus involved in sensory perception of bitter taste The series of events required for a bitter taste stimulus to be received and converted to a molecular signal.
lipid catabolic process The chemical reactions and pathways resulting in the breakdown of lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent.
phosphatidylinositol metabolic process The chemical reactions and pathways involving phosphatidylinositol, any glycophospholipid in which a sn-glycerol 3-phosphate residue is esterified to the 1-hydroxyl group of 1D-myo-inositol.
phosphatidylinositol-mediated signaling The series of molecular signals in which a cell uses a phosphatidylinositol-mediated signaling to convert a signal into a response. Phosphatidylinositols include phosphatidylinositol (PtdIns) and its phosphorylated derivatives.
phospholipase C-activating G protein-coupled receptor signaling pathway A G protein-coupled receptor signaling pathway in which the signal is transmitted via the activation of phospholipase C (PLC) and a subsequent increase in the intracellular concentration of inositol trisphosphate (IP3) and diacylglycerol (DAG).
sensory perception of bitter taste The series of events required to receive a bitter taste stimulus, convert it to a molecular signal, and recognize and characterize the signal. This is a neurological process.

24 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P32383 PLC1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P10894 PLCB1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 Bos taurus (Bovine) SS
Q9NQ66 PLCB1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 Homo sapiens (Human) EV
Q15147 PLCB4 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 Homo sapiens (Human) PR
Q01970 PLCB3 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Homo sapiens (Human) EV
Q00722 PLCB2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 Homo sapiens (Human) EV
Q8K394 Plcl2 Inactive phospholipase C-like protein 2 Mus musculus (Mouse) PR
Q8K2J0 Plcd3 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3 Mus musculus (Mouse) PR
Q62077 Plcg1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Mus musculus (Mouse) SS
P51432 Plcb3 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Mus musculus (Mouse) SS
Q9Z1B3 Plcb1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 Mus musculus (Mouse) SS
Q8K4S1 Plce1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 Mus musculus (Mouse) SS
Q8R3B1 Plcd1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 Mus musculus (Mouse) SS
P10687 Plcb1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 Rattus norvegicus (Rat) SS
Q99JE6 Plcb3 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Rattus norvegicus (Rat) SS
O89040 Plcb2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 Rattus norvegicus (Rat) PR
G5EBH0 egl-8 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 Caenorhabditis elegans SS
Q8GV43 PLC6 Phosphoinositide phospholipase C 6 Arabidopsis thaliana (Mouse-ear cress) PR
Q944C2 PLC5 Phosphoinositide phospholipase C 5 Arabidopsis thaliana (Mouse-ear cress) PR
Q6NMA7 PLC9 Phosphoinositide phospholipase C 9 Arabidopsis thaliana (Mouse-ear cress) PR
Q9STZ3 PLC8 Phosphoinositide phospholipase C 8 Arabidopsis thaliana (Mouse-ear cress) PR
Q56W08 PLC3 Phosphoinositide phospholipase C 3 Arabidopsis thaliana (Mouse-ear cress) PR
Q39032 PLC1 Phosphoinositide phospholipase C 1 Arabidopsis thaliana (Mouse-ear cress) PR
Q944C1 PLC4 Phosphoinositide phospholipase C 4 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MSLLNPVLLP PNVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT YQNQEMEFLD
70 80 90 100 110 120
VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTLT VVSGPDMVDL TFYNFVSYKE
130 140 150 160 170 180
NVGKDWAEDV LALAKHPMTV NAPRSTFLDK ILVKLKMQLN PEGKIPVKNF FQMFPADRKR
190 200 210 220 230 240
VEAALGACHL AKGKNDAINP EDFPESVYKS FLMSLCPRPE IDEIFTSYHS KAKPYMTKEH
250 260 270 280 290 300
LTKFINQKQR DPRLNSLLFP PARPEQVQVL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN
310 320 330 340 350 360
SVLAHDTLLI HQDMTQPLNH YFINSSHNTY LTAGQFSGLS SAEMYRQVLL SGCRCVELDC
370 380 390 400 410 420
WKGKPPDEEP IITHGFTMTT DILFKEAIEA IAESAFKTSP YPVILSFENH VDSPRQQAKM
430 440 450 460 470 480
AEYCRSMFGE TLLTDPLENF PLKPGIPLPS PEDLRGKILI KNKKNQFSGP ASPSKKPGGV
490 500 510 520 530 540
AEGSLPSSVP VEEDTGWTAE DRTEVEEEEV VEEEEEEESG NLDEEEIKKM QSDEGTAGLE
550 560 570 580 590 600
VTAYEEMSSL VNYIQPTKFI SFEFSAQKNR SYVVSSFTEL KAYELLSKAS MQFVDYNKRQ
610 620 630 640 650 660
MSRVYPKGTR MDSSNYMPQM FWNAGCQMVA LNFQTMDLPM QQNMALFEFN GQSGYLLKHE
670 680 690 700 710 720
FMRRLDKQFN PFSVDRIDVV VATTLSITII SGQFLSERSV RTYVEVELFG LPGDPKRRYR
730 740 750 760 770 780
TKLSPTANSI NPVWKEEPFI FEKILMPELA SLRIAVMEEG SKFLGHRIIP INALHSGYHH
790 800 810 820 830 840
LCLRSESNMA LTMPALFVFL EMKDYIPDTW ADLTVALANP IKYFNAQDKK SVKLKGVTGS
850 860 870 880 890 900
LPEKLFSGTP VASQSNGAPV SAGNGSTAPG TKATGEEATK EVTEPQTASL EELRELKGVV
910 920 930 940 950 960
KLQRRHEKEL RELERRGARR WEELLQRGAA QLAELQTQAA GCKLRPGKGS RKKRTLPCEE
970 980 990 1000 1010 1020
TVVAPSEPHD RADPRVQELK DRLEQELQQQ GEEQYRSVLK RKEQHVTEQI AKMMELAREK
1030 1040 1050 1060 1070 1080
QAAELKTFKE TSETDTKEMK KKLEAKRLER IQAMTKVTTD KVAQERLKRE INNSHIQEVV
1090 1100 1110 1120 1130 1140
QAVKQMTETL ERHQEKLEER QTACLEQIQA MEKQFQEKAL AEYEAKMKGL EAEVKESVRA
1150 1160 1170 1180
YFKDCFPTEA EDKPERSCEA SEESCPQEPL VSKADTQESR L