A2VE08
SS
Gene name |
KPNA1 |
Protein name |
Importin subunit alpha-5 |
Names |
Karyopherin subunit alpha-1 [Cleaved into: Importin subunit alpha-5, N-terminally processed] |
Species |
Bos taurus (Bovine) |
KEGG Pathway |
bta:539679 |
EC number |
|
Protein Class |
|
Descriptions
Importin-α is a nuclear import receptor that facilitates the transport of proteins with nuclear localization sequences (NLSs) into the nucleus. It is autoinhibited by its N-terminal region, which occupies the NLS-binding site, preventing unnecessary binding and import activity. Importin-α is autoinhibited in the absence of importin-β and the binding of importin-β alleviates this autoinhibition, which displaces the autoinhibitory sequence.
Autoinhibitory domains (AIDs)
Target domain |
149-241 (Major NLS-binding site);318-406 (Minor NLS-binding site) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Fontes MR et al. (2000) "Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-alpha", Journal of molecular biology, 297, 1183-94
- Fontes MR et al. (2003) "Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha", The Journal of biological chemistry, 278, 27981-7
- Catimel B et al. (2001) "Biophysical characterization of interactions involving importin-alpha during nuclear import", The Journal of biological chemistry, 276, 34189-98
- Pumroy RA et al. (2015) "Molecular determinants for nuclear import of influenza A PB2 by importin α isoforms 3 and 7", Structure (London, England : 1993), 23, 374-84
- Kimoto C et al. (2015) "Functional characterization of importin α8 as a classical nuclear localization signal receptor", Biochimica et biophysica acta, 1853, 2676-83
- Miyatake H et al. (2015) "Crystal structure of human importin-α1 (Rch1), revealing a potential autoinhibition mode involving homodimerization", PloS one, 10, e0115995
- Kobe B (1999) "Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin alpha", Nature structural biology, 6, 388-97
Autoinhibited structure
Activated structure
1 structures for A2VE08
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-A2VE08-F1 | Predicted | AlphaFoldDB |
28 variants for A2VE08
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs466712486 | 18 | S>F | No | EVA | |
| rs450006962 | 100 | L>I | No | EVA | |
| rs481189607 | 101 | S>P | No | EVA | |
| rs444087068 | 143 | L>P | No | EVA | |
| rs475453594 | 144 | Q>E | No | EVA | |
| rs449855377 | 150 | V>G | No | EVA | |
| rs461210246 | 155 | A>G | No | EVA | |
| rs442028642 | 157 | G>A | No | EVA | |
| rs473372376 | 164 | I>T | No | EVA | |
| rs459889107 | 179 | S>R | No | EVA | |
| rs439686959 | 187 | E>K | No | EVA | |
| rs470860671 | 188 | Q>P | No | EVA | |
| rs876093815 | 201 | T>S | No | EVA | |
| rs453858495 | 209 | D>E | No | EVA | |
| rs433741504 | 210 | C>S | No | EVA | |
| rs477724312 | 266 | S>C | No | EVA | |
| rs457651925 | 305 | L>S | No | EVA | |
| rs444476285 | 306 | M>I | No | EVA | |
| rs462293969 | 307 | H>P | No | EVA | |
| rs482263904 | 307 | H>Y | No | EVA | |
| rs442090425 | 316 | A>S | No | EVA | |
| rs474353879 | 367 | A>G | No | EVA | |
| rs465365487 | 394 | F>L | No | EVA | |
| rs472256176 | 418 | Y>S | No | EVA | |
| rs458678116 | 486 | S>I | No | EVA | |
| rs438488206 | 497 | F>V | No | EVA | |
| rs456335520 | 517 | V>L | No | EVA | |
| rs442568932 | 536 | F>S | No | EVA |
No associated diseases with A2VE08
9 regional properties for A2VE08
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| repeat | Armadillo | 114 - 198 | IPR000225-1 |
| repeat | Armadillo | 200 - 241 | IPR000225-2 |
| repeat | Armadillo | 244 - 283 | IPR000225-3 |
| repeat | Armadillo | 285 - 325 | IPR000225-4 |
| repeat | Armadillo | 327 - 369 | IPR000225-5 |
| repeat | Armadillo | 369 - 409 | IPR000225-6 |
| repeat | Armadillo | 412 - 452 | IPR000225-7 |
| domain | Importin-alpha, importin-beta-binding domain | 1 - 104 | IPR002652 |
| repeat | Atypical Arm repeat | 466 - 515 | IPR032413 |
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| dendrite | A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body. |
| nucleoplasm | That part of the nuclear content other than the chromosomes or the nucleolus. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| synapse | The junction between an axon of one neuron and a dendrite of another neuron, a muscle fiber or a glial cell. As the axon approaches the synapse it enlarges into a specialized structure, the presynaptic terminal bouton, which contains mitochondria and synaptic vesicles. At the tip of the terminal bouton is the presynaptic membrane; facing it, and separated from it by a minute cleft (the synaptic cleft) is a specialized area of membrane on the receiving cell, known as the postsynaptic membrane. In response to the arrival of nerve impulses, the presynaptic terminal bouton secretes molecules of neurotransmitters into the synaptic cleft. These diffuse across the cleft and transmit the signal to the postsynaptic membrane. |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| nuclear import signal receptor activity | Combining with a nuclear import signal (NIS) on a cargo to be transported, to mediate transport of the cargo through the nuclear pore, from the cytoplasm to the nuclear lumen. The cargo can be either a RNA or a protein. |
| nuclear localization sequence binding | Binding to a nuclear localization sequence, a specific peptide sequence that acts as a signal to localize the protein within the nucleus. |
2 GO annotations of biological process
| Name | Definition |
|---|---|
| NLS-bearing protein import into nucleus | The directed movement of a protein bearing a nuclear localization signal (NLS) from the cytoplasm into the nucleus, across the nuclear envelope. |
| postsynapse to nucleus signaling pathway | The series of molecular signals that conveys information from the postsynapse to the nucleus via cytoskeletal transport of a protein from a postsynapse to the component to the nucleus where it affects biochemical processes that occur in the nucleus (e.g DNA transcription, mRNA splicing, or DNA/histone modifications). |
30 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q02821 | SRP1 | Importin subunit alpha | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | SS |
| C1JZ66 | KPNA7 | Importin subunit alpha-8 | Bos taurus (Bovine) | PR |
| Q0V7M0 | KPNA6 | Importin subunit alpha-7 | Bos taurus (Bovine) | SS |
| Q5ZML1 | KPNA1 | Importin subunit alpha-5 | Gallus gallus (Chicken) | SS |
| P52295 | Pen | Importin subunit alpha | Drosophila melanogaster (Fruit fly) | SS |
| O00505 | KPNA3 | Importin subunit alpha-4 | Homo sapiens (Human) | SS |
| A9QM74 | KPNA7 | Importin subunit alpha-8 | Homo sapiens (Human) | EV |
| O00629 | KPNA4 | Importin subunit alpha-3 | Homo sapiens (Human) | SS |
| P52292 | KPNA2 | Importin subunit alpha-1 | Homo sapiens (Human) | EV |
| O15131 | KPNA5 | Importin subunit alpha-6 | Homo sapiens (Human) | SS |
| O60684 | KPNA6 | Importin subunit alpha-7 | Homo sapiens (Human) | SS |
| P52294 | KPNA1 | Importin subunit alpha-5 | Homo sapiens (Human) | SS |
| P52293 | Kpna2 | Importin subunit alpha-1 | Mus musculus (Mouse) | EV |
| O35344 | Kpna3 | Importin subunit alpha-4 | Mus musculus (Mouse) | SS |
| O35343 | Kpna4 | Importin subunit alpha-3 | Mus musculus (Mouse) | SS |
| C0LLJ0 | Kpna7 | Importin subunit alpha-8 | Mus musculus (Mouse) | SS |
| O35345 | Kpna6 | Importin subunit alpha-7 | Mus musculus (Mouse) | PR |
| Q60960 | Kpna1 | Importin subunit alpha-5 | Mus musculus (Mouse) | SS |
| C6K7I2 | KPNA7 | Importin subunit alpha-8 | Sus scrofa (Pig) | SS |
| Q56R16 | Kpna5 | Importin subunit alpha-6 | Rattus norvegicus (Rat) | SS |
| P83953 | Kpna1 | Importin subunit alpha-5 | Rattus norvegicus (Rat) | SS |
| Q71VM4 | Os01g0253300 | Importin subunit alpha-1a | Oryza sativa subsp. japonica (Rice) | SS |
| P91276 | ima-2 | Importin subunit alpha-2 | Caenorhabditis elegans | SS |
| Q19969 | ima-3 | Importin subunit alpha-3 | Caenorhabditis elegans | SS |
| O04294 | IMPA3 | Importin subunit alpha-3 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FJ09 | IMPA5 | Importin subunit alpha-5 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9M9X7 | IMPA7 | Importin subunit alpha-7 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FWY7 | IMPA6 | Importin subunit alpha-6 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| O22478 | Importin subunit alpha | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) | SS | |
| Q503E9 | kpna5 | Importin subunit alpha-6 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MTTPGKENFR | LKSYKNKSLN | PDEMRRRREE | EGLQLRKQKR | EEQLFKRRNV | ATAEEETEEE |
| 70 | 80 | 90 | 100 | 110 | 120 |
| VMSDGGFHEA | QINNMEMAPG | GVITSDMIEM | IFSNSPEQQL | SATQKFRKLL | SKEPNPPIDE |
| 130 | 140 | 150 | 160 | 170 | 180 |
| VISTPGVVAR | FVEFLKRKEN | CTLQFESAWV | LTNIASGNSL | QTRIVIQAGA | VPIFIELLSS |
| 190 | 200 | 210 | 220 | 230 | 240 |
| EFEDVQEQAV | WALGNIAGDS | TMCRDYVLDC | NILPPLLQLF | SKQNRLTMTR | NAVWALSNLC |
| 250 | 260 | 270 | 280 | 290 | 300 |
| RGKSPPPEFA | KVSPCLNVLS | WLLFVSDTDV | LADACWALSY | LSDGPNDKIQ | AVIDAGVCRR |
| 310 | 320 | 330 | 340 | 350 | 360 |
| LVELLMHNDY | KVVSPALRAV | GNIVTGDDVQ | TQVILNCSAL | QSLLHLLSSP | KESIKKEACW |
| 370 | 380 | 390 | 400 | 410 | 420 |
| TISNITAGNR | AQIQTVIDAN | IFPALISILQ | TAEFRTRKEA | AWAITNATSG | GSAEQIKYLV |
| 430 | 440 | 450 | 460 | 470 | 480 |
| ELGCIKPLCD | LLTVMDSKIV | QVALNGLENI | LRLGEQEAKR | NGTGINPYCA | LIEEAYGLDK |
| 490 | 500 | 510 | 520 | 530 | |
| IEFLQSHENQ | EIYQKAFDLI | EHYFGTEDED | SSIAPQVDLS | QQQYIFQQCE | APMEGFQL |