A1Z9X0

SS
Gene name
aPKC (CG30475)
Protein name
Atypical protein kinase C
Names
EC 2.7.11.13
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG42783
EC number
2.7.11.13: Protein-serine/threonine kinases
Protein Class

Descriptions

PRKCI, an atypical PKC (aPKC) isoform, is an important kinase in multiple cellular processes and regulated by the phosphatidylinositol 3-kinase product phosphatidylinositol 3,4,5-(PO4)3 (PIP3). In aPKC molecule, the arginine motif (123-140) in the pseudosubstrate region on the regulatory domain binds to acidic residues in the substrate-binding region on the catalytic domain, leading to the off state of aPKCs. The autoinhibition of aPKC is released by binding with the acidic ligand PIP3 and consequently exposing the substrate-binding site.

Autoinhibitory domains (AIDs)

128-145 (Pseudosubstrate region) SS

Target domain

237-606 (Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C iota)

Relief mechanism

Ligand binding

Assay

Accessory elements

405-428 (Activation loop from InterPro)

Target domain

268-595 (Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C)

Relief mechanism

Assay

405-428 (Activation loop from InterPro)

Target domain

264-595 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for A1Z9X0

Entry ID Method Resolution Chain Position Source
AF-A1Z9X0-F1 Predicted AlphaFoldDB

No variants for A1Z9X0

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for A1Z9X0

No associated diseases with A1Z9X0

4 regional properties for A1Z9X0

Type Name Position InterPro Accession
domain Protein kinase domain 14 - 272 IPR000719
active_site Serine/threonine-protein kinase, active site 132 - 144 IPR008271
domain Calcium/calmodulin-dependent protein kinase II, association-domain 356 - 483 IPR013543
binding_site Protein kinase, ATP binding site 20 - 43 IPR017441

Functions

Description
EC Number 2.7.11.13 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm, cell cortex
  • Apicolateral cell membrane ; Peripheral membrane protein
  • Cytoplasmic at interphase but localizes to the apical cell cortex during mitosis
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
apical cortex The region that lies just beneath the plasma membrane on the apical edge of a cell.
apical junction complex A functional unit located near the cell apex at the points of contact between epithelial cells, which in vertebrates is composed of the tight junction, the zonula adherens, and desmosomes and in some invertebrates, such as Drosophila, is composed of the subapical complex (SAC), the zonula adherens and the septate junction. Functions in the regulation of cell polarity, tissue integrity and intercellular adhesion and permeability.
apical plasma membrane The region of the plasma membrane located at the apical end of the cell.
apicolateral plasma membrane The apical end of the lateral plasma membrane of epithelial cells.
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
subapical complex The most apical region of the lateral plasma membrane of an invertebrate epithelial cell. The subapical complex lies above the zonula adherens and the septate junction, and is comparable to the position of the tight junction of vertebrate cells.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
diacylglycerol-dependent serine/threonine kinase activity Catalysis of the reaction
metal ion binding Binding to a metal ion.
myosin binding Binding to a myosin; myosins are any of a superfamily of molecular motor proteins that bind to actin and use the energy of ATP hydrolysis to generate force and movement along actin filaments.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

39 GO annotations of biological process

Name Definition
adherens junction organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of an adherens junction. An adherens junction is a cell-cell junction composed of the epithelial cadherin-catenin complex at which the cytoplasmic face of the plasma membrane is attached to actin filaments.
apical protein localization Any process in which a protein is transported to, or maintained in, apical regions of the cell.
asymmetric neuroblast division The process resulting in the physical partitioning and separation of a neuroblast into two daughter cells with different developmental potentials.
asymmetric protein localization involved in cell fate determination Any process in which a protein is transported to, or maintained in, a specific asymmetric distribution, resulting in the formation of daughter cells of different types.
branching involved in open tracheal system development The process in which the anatomical structures of branches in the open tracheal system are generated and organized.
cell-cell junction assembly The aggregation, arrangement and bonding together of a set of components to form a junction between cells.
compound eye retinal cell programmed cell death Programmed cell death that occurs in the retina to remove excess cells between ommatidia, thus resulting in a hexagonal lattice, precise with respect to cell number and position surrounding each ommatidium.
epithelial cell morphogenesis The change in form that occurs when an epithelial cell progresses from its initial formation to its mature state.
establishment of epithelial cell apical/basal polarity The specification and formation of the apicobasal polarity of an epithelial cell.
establishment of epithelial cell planar polarity The specification and formation of the polarity of an epithelial cell along the plane of the epithelial tissue.
establishment of mitotic spindle orientation A cell cycle process that sets the alignment of mitotic spindle relative to other cellular structures.
establishment or maintenance of cell polarity Any cellular process that results in the specification, formation or maintenance of anisotropic intracellular organization or cell growth patterns.
establishment or maintenance of epithelial cell apical/basal polarity Any cellular process that results in the specification, formation or maintenance of the apicobasal polarity of an epithelial cell.
establishment or maintenance of neuroblast polarity Any cellular process that results in the specification, formation or maintenance of the apicobasal polarity of a neuroblast cell, a progenitor of the central nervous system.
establishment or maintenance of polarity of embryonic epithelium Any cellular process that results in the specification, formation or maintenance of anisotropic intracellular organization of epithelial cells in an embryo.
establishment or maintenance of polarity of follicular epithelium Any cellular process that results in the specification, formation or maintenance of a polarized follicular epithelial sheet.
exocyst localization Any process in which an exocyst is transported to, or maintained in, a specific location. An exocyst is a protein complex peripherally associated with the plasma membrane that determines where vesicles dock and fuse.
germarium-derived oocyte fate determination The cell fate determination process in which a germarium-derived cell becomes capable of differentiating autonomously into an oocyte cell regardless of its environment; upon determination, the cell fate cannot be reversed. An example of this is found in Drosophila melanogaster.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
maintenance of cell polarity The maintenance of established anisotropic intracellular organization or cell growth patterns.
melanotic encapsulation of foreign target Formation of a multilayered, melanized sheath of cells around a foreign invader.
memory The activities involved in the mental information processing system that receives (registers), modifies, stores, and retrieves informational stimuli. The main stages involved in the formation and retrieval of memory are encoding (processing of received information by acquisition), storage (building a permanent record of received information as a result of consolidation) and retrieval (calling back the stored information and use it in a suitable way to execute a given task).
morphogenesis of a polarized epithelium The morphogenetic process in which the anatomical structures of a polarized epithelium are generated and organized. A polarized epithelium is an epithelium where the epithelial sheet is oriented with respect to the planar axis.
negative regulation of hippo signaling Any process that stops, prevents, or reduces the frequency, rate or extent of hippo signaling.
nuclear envelope budding The process by which large macromolecular complexes are budded through the inner nuclear membrane, into the perinuclear space, thus acquiring a membrane envelope. The enveloped particle fuses with the outer nuclear membrane and is released into the cytoplasm.
oocyte anterior/posterior axis specification Polarization of the oocyte along its anterior-posterior axis. An example of this is found in Drosophila melanogaster.
positive regulation of filopodium assembly Any process that activates or increases the frequency, rate or extent of the assembly of a filopodium, a thin, stiff protrusion extended by the leading edge of a motile cell such as a crawling fibroblast or amoeba, or an axonal growth cone.
positive regulation of lamellipodium assembly Any process that increases the rate, frequency or extent of the formation of a lamellipodium, a thin sheetlike extension of the surface of a migrating cell.
positive regulation of neuroblast proliferation Any process that activates or increases the rate of neuroblast proliferation.
positive regulation of smoothened signaling pathway Any process that activates or increases the frequency, rate or extent of smoothened signaling.
protein localization to plasma membrane A process in which a protein is transported to, or maintained in, a specific location in the plasma membrane.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of intracellular mRNA localization Any process that modulates the frequency, rate or extent of intracellular mRNA localization.
regulation of polarized epithelial cell differentiation Any process that modulates the frequency, rate or extent of polarized epithelial cell differentiation.
sensory organ development The process whose specific outcome is the progression of sensory organs over time, from its formation to the mature structure.
spermatogenesis The developmental process by which male germ line stem cells self renew or give rise to successive cell types resulting in the development of a spermatozoa.
synapse assembly The aggregation, arrangement and bonding together of a set of components to form a synapse. This process ends when the synapse is mature (functional).
terminal branching, open tracheal system Formation of terminal branches in the open tracheal system. These are long cytoplasmic extensions that form fine tubules that transport oxygen directly to the tissues. An example of the process is found in Drosophila melanogaster.
zonula adherens assembly Assembly of the zonula adherens, a cell-cell adherens junction which forms a continuous belt near the apex of epithelial cells.

16 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P24583 PKC1 Protein kinase C-like 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SS
Q9W0V1 Pdk1 3-phosphoinositide-dependent protein kinase 1 Drosophila melanogaster (Fruit fly) SS
A1Z7T0 Pkn Serine/threonine-protein kinase N Drosophila melanogaster (Fruit fly) SS
P83099 Pkcdelta Putative protein kinase C delta type homolog Drosophila melanogaster (Fruit fly) PR
P13677 inaC Protein kinase C, eye isozyme Drosophila melanogaster (Fruit fly) SS
P05130 Pkc53E Protein kinase C, brain isozyme Drosophila melanogaster (Fruit fly) SS
Q9NBK5 trc Serine/threonine-protein kinase tricornered Drosophila melanogaster (Fruit fly) SS
Q8INB9 Akt RAC serine/threonine-protein kinase Drosophila melanogaster (Fruit fly) SS
Q05513 PRKCZ Protein kinase C zeta type Homo sapiens (Human) SS
P41743 PRKCI Protein kinase C iota type Homo sapiens (Human) EV
Q02956 Prkcz Protein kinase C zeta type Mus musculus (Mouse) SS
Q62074 Prkci Protein kinase C iota type Mus musculus (Mouse) SS
P09217 Prkcz Protein kinase C zeta type Rattus norvegicus (Rat) SS
F1M7Y5 Prkci Protein kinase C iota type Rattus norvegicus (Rat) SS
Q19266 pkc-3 Protein kinase C-like 3 Caenorhabditis elegans SS
Q90XF2 prkci Protein kinase C iota type Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MQKMPSQILN DGSSVSLNSA SMNMANTPNS ITVKTAYNGQ IIITTINKNI SYEELCYEIR
70 80 90 100 110 120
NICRFPLDQP FTIKWVDEEN DPCTISTKME LDEAIRLYEM NFDSQLVIHV FPNVPQAPGL
130 140 150 160 170 180
SCDGEDRSIY RRGARRWRKL YRVNGHIFQA KRFNRRAFCA YCQDRIWGLG RQGFKCIQCK
190 200 210 220 230 240
LLVHKKCHKL VQKHCTDQPE PLVKERAEES SDPIPVPLPP LPYEAMSGGA EACETHDHAH
250 260 270 280 290 300
IVAPPPPEDP LEPGTQRQYS LNDFELIRVI GRGSYAKVLM VELRRTRRIY AMKVIKKALV
310 320 330 340 350 360
TDDEDIDWVQ TEKHVFETAS NHPFLVGLHS CFQTPSRLFF VIEFVRGGDL MYHMQRQRRL
370 380 390 400 410 420
PEEHARFYAA EISLALNFLH EKGIIYRDLK LDNVLLDHEG HIKLTDYGMC KEGIRPGDTT
430 440 450 460 470 480
STFCGTPNYI APEILRGEDY GFSVDWWALG VLLYEMLAGR SPFDLAGASE NPDQNTEDYL
490 500 510 520 530 540
FQVILEKTIR IPRSLSVRAA SVLKGFLNKN PADRLGCHRE SAFMDIVSHP FFKNMDWELL
550 560 570 580 590 600
ERKQVTPPFK PRLDSDRDLA NFPPEFTGEA VQLTPDDDHV IDNIDQSEFE GFEYVNPLLM
SLEDCV