A1Z7T0
SS
Gene name |
Pkn (Dpkn, CG2049) |
Protein name |
Serine/threonine-protein kinase N |
Names |
EC 2.7.11.13 , Protein kinase related to PKN |
Species |
Drosophila melanogaster (Fruit fly) |
KEGG Pathway |
dme:Dmel_CG2049 |
EC number |
2.7.11.13: Protein-serine/threonine kinases |
Protein Class |
|
Descriptions
Serine/threonine-protein kinase N1 (PKN1) is a PKC-related serine/threonine protein kinase involved in specific signal transduction responses such as regulation of intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion, and transcription regulation in cells. Its kinase activity is enhanced by fatty acids such as arachidonic acid or the small GTP-binding protein Rho. Residues 455-511 of PKN selectively inhibit the kinase activity of the catalytic fragment of PKN1. Arachidonic acid relieves the catalytic activity of wild-type PKN from autoinhibition by residues 455-511 of PKN.
Autoinhibitory domains (AIDs)
Target domain |
863-1122 (Protein kinase domain) |
Relief mechanism |
Ligand binding, PTM |
Assay |
|
Accessory elements
No accessory elements
References
- Yoshinaga C et al. (1999) "Mutational analysis of the regulatory mechanism of PKN: the regulatory region of PKN contains an arachidonic acid-sensitive autoinhibitory domain", Journal of biochemistry, 126, 475-84
- Huang X et al. (2003) "Crystal structure of an inactive Akt2 kinase domain", Structure (London, England : 1993), 11, 21-30
- Truebestein L et al. (2021) "Structure of autoinhibited Akt1 reveals mechanism of PIP(3)-mediated activation", Proceedings of the National Academy of Sciences of the United States of America, 118,
- Lučić I et al. (2018) "Conformational sampling of membranes by Akt controls its activation and inactivation", Proceedings of the National Academy of Sciences of the United States of America, 115, E3940-E3949
Autoinhibited structure
Activated structure
1 structures for A1Z7T0
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-A1Z7T0-F1 | Predicted | AlphaFoldDB |
No variants for A1Z7T0
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for A1Z7T0 | |||||
No associated diseases with A1Z7T0
10 regional properties for A1Z7T0
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | C2 domain | 359 - 515 | IPR000008 |
| domain | Protein kinase domain | 863 - 1122 | IPR000719 |
| domain | AGC-kinase, C-terminal | 1123 - 1190 | IPR000961 |
| active_site | Serine/threonine-protein kinase, active site | 984 - 996 | IPR008271 |
| domain | HR1 rho-binding domain | 28 - 103 | IPR011072-1 |
| domain | HR1 rho-binding domain | 142 - 220 | IPR011072-2 |
| domain | HR1 rho-binding domain | 242 - 323 | IPR011072-3 |
| binding_site | Protein kinase, ATP binding site | 869 - 892 | IPR017441 |
| domain | Protein kinase, C-terminal | 1143 - 1185 | IPR017892 |
| domain | Serine/threonine-protein kinase N, first HR1 domain | 37 - 100 | IPR037313 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.11.13 | Protein-serine/threonine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
9 GO annotations of cellular component
| Name | Definition |
|---|---|
| anchoring junction | A cell junction that mechanically attaches a cell (and its cytoskeleton) to neighboring cells or to the extracellular matrix. |
| apical cortex | The region that lies just beneath the plasma membrane on the apical edge of a cell. |
| cleavage furrow | The cleavage furrow is a plasma membrane invagination at the cell division site. The cleavage furrow begins as a shallow groove and eventually deepens to divide the cytoplasm. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytoskeleton | A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| lamellipodium | A thin sheetlike process extended by the leading edge of a migrating cell or extending cell process; contains a dense meshwork of actin filaments. |
| midbody | A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
6 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| diacylglycerol-dependent serine/threonine kinase activity | Catalysis of the reaction |
| protein kinase activity | Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction |
| protein serine kinase activity | Catalysis of the reactions |
| protein serine/threonine kinase activity | Catalysis of the reactions |
| small GTPase binding | Binding to a small monomeric GTPase. |
8 GO annotations of biological process
| Name | Definition |
|---|---|
| actin cytoskeleton organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins. |
| cell adhesion | The attachment of a cell, either to another cell or to an underlying substrate such as the extracellular matrix, via cell adhesion molecules. |
| dorsal closure | The process during Drosophila embryogenesis whereby the ectodermal cells of the lateral epithelium stretch in a coordinated fashion to internalize the amnioserosa cells and close the embryo dorsally. |
| intracellular signal transduction | The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell. |
| mitotic cytokinesis, division site positioning | The process in which a contractile ring is positioned in a specific location during the mitotic cell cycle. This process is critical for both for both symmetric and asymmetric cell divisions. |
| positive regulation of wound healing | Any process that increases the rate, frequency, or extent of the series of events that restore integrity to a damaged tissue, following an injury. |
| protein autophosphorylation | The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation). |
| wing disc morphogenesis | The process in which the anatomical structures derived from the wing disc are generated and organized. This includes the transformation of a wing imaginal disc from a monolayered epithelium in the larvae of holometabolous insects into recognizable adult structures including the wing hinge, wing blade and pleura. |
31 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P24583 | PKC1 | Protein kinase C-like 1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | SS |
| A1A4I4 | PKN1 | Serine/threonine-protein kinase N1 | Bos taurus (Bovine) | SS |
| Q9W0V1 | Pdk1 | 3-phosphoinositide-dependent protein kinase 1 | Drosophila melanogaster (Fruit fly) | SS |
| A1Z9X0 | aPKC | Atypical protein kinase C | Drosophila melanogaster (Fruit fly) | SS |
| P13677 | inaC | Protein kinase C, eye isozyme | Drosophila melanogaster (Fruit fly) | SS |
| P05130 | Pkc53E | Protein kinase C, brain isozyme | Drosophila melanogaster (Fruit fly) | SS |
| Q8INB9 | Akt | RAC serine/threonine-protein kinase | Drosophila melanogaster (Fruit fly) | SS |
| Q9NBK5 | trc | Serine/threonine-protein kinase tricornered | Drosophila melanogaster (Fruit fly) | SS |
| P83099 | Pkcdelta | Putative protein kinase C delta type homolog | Drosophila melanogaster (Fruit fly) | PR |
| Q04759 | PRKCQ | Protein kinase C theta type | Homo sapiens (Human) | PR |
| Q96LW2 | RSKR | Ribosomal protein S6 kinase-related protein | Homo sapiens (Human) | PR |
| Q02156 | PRKCE | Protein kinase C epsilon type | Homo sapiens (Human) | SS |
| P24723 | PRKCH | Protein kinase C eta type | Homo sapiens (Human) | SS |
| Q05655 | PRKCD | Protein kinase C delta type | Homo sapiens (Human) | SS |
| Q16512 | PKN1 | Serine/threonine-protein kinase N1 | Homo sapiens (Human) | EV |
| Q16513 | PKN2 | Serine/threonine-protein kinase N2 | Homo sapiens (Human) | EV |
| Q6P5Z2 | PKN3 | Serine/threonine-protein kinase N3 | Homo sapiens (Human) | SS |
| P23298 | Prkch | Protein kinase C eta type | Mus musculus (Mouse) | PR |
| Q02111 | Prkcq | Protein kinase C theta type | Mus musculus (Mouse) | PR |
| P16054 | Prkce | Protein kinase C epsilon type | Mus musculus (Mouse) | PR |
| P28867 | Prkcd | Protein kinase C delta type | Mus musculus (Mouse) | PR |
| P70268 | Pkn1 | Serine/threonine-protein kinase N1 | Mus musculus (Mouse) | SS |
| Q8BWW9 | Pkn2 | Serine/threonine-protein kinase N2 | Mus musculus (Mouse) | SS |
| Q8K045 | Pkn3 | Serine/threonine-protein kinase N3 | Mus musculus (Mouse) | SS |
| Q64617 | Prkch | Protein kinase C eta type | Rattus norvegicus (Rat) | PR |
| P09216 | Prkce | Protein kinase C epsilon type | Rattus norvegicus (Rat) | PR |
| P09215 | Prkcd | Protein kinase C delta type | Rattus norvegicus (Rat) | PR |
| Q63433 | Pkn1 | Serine/threonine-protein kinase N1 | Rattus norvegicus (Rat) | SS |
| O08874 | Pkn2 | Serine/threonine-protein kinase N2 | Rattus norvegicus (Rat) | SS |
| P34722 | tpa-1 | Protein kinase C-like 1 | Caenorhabditis elegans | PR |
| A7MBL8 | pkn2 | Serine/threonine-protein kinase N2 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSDSYYQGEY | IKHPVLYELS | HKYGFTENLP | ESCMSIRLEE | IKEAIRREIR | KELKIKEGAE |
| 70 | 80 | 90 | 100 | 110 | 120 |
| KLREVAKDRR | SLSDVAVLVK | KSKSKLAELK | SELQELESQI | LLTSANTAVN | SNGQESITAC |
| 130 | 140 | 150 | 160 | 170 | 180 |
| IDPNGGFLVS | GAVGGLGGGN | TALEGGAPAT | ANDKVLASLE | KQLQIEMKVK | TGAENMIQSL |
| 190 | 200 | 210 | 220 | 230 | 240 |
| GIGCDKKLLA | EAHQMLADSK | AKIEFLRLRI | IKVKQNREQA | DRLKASRQMI | DEHGQTIGGN |
| 250 | 260 | 270 | 280 | 290 | 300 |
| NSSQPQSLET | TLEERIEELR | HRLRIEAAVV | DGAKNVIRTL | QTANRAPDKK | ALQEAHGRLS |
| 310 | 320 | 330 | 340 | 350 | 360 |
| ESSRKLDLLR | YSLDLRRQEL | PADSPAAQQL | KTELQIVQLS | TSPAPVTYTS | LQSGQAGILG |
| 370 | 380 | 390 | 400 | 410 | 420 |
| GKPYQSVSSL | GRCASVTGKL | EVRLLGCQDL | LEDVPGRSRR | DKDNNSSPGD | LRSFVKGVTS |
| 430 | 440 | 450 | 460 | 470 | 480 |
| RSSSKSYSVK | DETSIEIMAV | IKLDNITVGQ | TSWKQCSQQA | WDQRFSIDLD | RSRELEIGVY |
| 490 | 500 | 510 | 520 | 530 | 540 |
| WRDWRSLCAV | KVLRLEEFID | DVRHGMALQL | EPQGLLFAEV | KFLNPMISQK | PKLRRQRMIF |
| 550 | 560 | 570 | 580 | 590 | 600 |
| NRQQAKNISR | AKQMNINVAT | WGRLLKRNAP | NHVHMGSAGS | GSSLTGSSPM | VVGGSRDSES |
| 610 | 620 | 630 | 640 | 650 | 660 |
| PISRTPSSDA | LVEPEPYTPG | EQAQNLEFDP | DAGINEHVET | PGEYPDPAAS | GLSGMRPLSM |
| 670 | 680 | 690 | 700 | 710 | 720 |
| HMQGISVLPP | ESPPVATGAA | GRPNTLSLQM | PGASKGQVIQ | GGRTAAPTTA | PPPPPVLKAT |
| 730 | 740 | 750 | 760 | 770 | 780 |
| STTPILDQEV | IPQLGKLYVG | SSQQQYAQQS | SPIIQEPATP | TIYGNSAAAG | APQFPQPAQR |
| 790 | 800 | 810 | 820 | 830 | 840 |
| QEKQPPQQQP | IYANQYELNV | AKAAAAASVY | SPSSSTTSNS | NQQQQQQRRN | VARGLQYRES |
| 850 | 860 | 870 | 880 | 890 | 900 |
| GGLETGRAGK | QPPNAGMLSM | DNFRLLSVLG | RGHFGKVILS | QLRSNNQYYA | IKALKKGDII |
| 910 | 920 | 930 | 940 | 950 | 960 |
| ARDEVESLLS | EKRIFEVANA | MRHPFLVNLY | SCFQTEQHVC | FVMEYAAGGD | LMMHIHTDVF |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| LEPRAVFYAA | CVVLGLQYLH | ENKIIYRDLK | LDNLLLDTEG | YVKIADFGLC | KEGMGFGDRT |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| GTFCGTPEFL | APEVLTETSY | TRAVDWWGLG | VLIFEMLVGE | SPFPGDDEEE | VFDSIVNDEV |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| RYPRFLSLEA | IAVMRRLLRK | NPERRLGSSE | RDAEDVKKQA | FFRSIVWDDL | LLRKVKPPFV |
| 1150 | 1160 | 1170 | 1180 | ||
| PTINHLEDVS | NFDEEFTSEK | AQLTPPKEPR | HLTEEEQLLF | QDFSYTAEWC |